Construction of novel nonenzymatic Xanthine biosensor based on reduced graphene oxide/polypyrrole/CdO nanocomposite for fish meat freshness detection
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A novel nonenzymatic voltammetric Xanthine biosensor was constructed based on a three-dimensional porous nanocomposite of reduced graphene oxide/polypyrrole/CdO nanocomposite modified glassy carbon electrode (GCE/rGO/PPy/CdO) for measuring of Xanthine. The structure and morphology of rGO/PPy/CdO nanocomposites were characterized by field emission scanning microscopy, Raman spectroscopy, X-ray diffraction, UV–vis spectroscopy, Fourier transform infrared and X-ray photoelectron spectroscopy. The GCE/rGO/PPy/CdO based biosensor exhibited excellent electrocatalytic activity and high stability for Xanthine oxidation. Under optimized conditions, the linearity between the current response and the Xanthine concentration was obtained in the range of 1–800 µM with a detection limit of 0.11 μM (S/N = 3). The biosensor was used to determine the Xanthine in fish meat with satisfactory results.
KeywordsNonenzymatic biosensor Xanthine Reduced graphene oxide CdO nanostructures Polypyrrole
The authors gratefully acknowledge partial financial support from the Research Council of Alzahra University.
- 5.V.K. Gupta, H. Karimi-Maleh, R. Sadegh, Simultaneous determination of hydroxylamine, phenol and sulfite in water and waste water samples using a voltammetric nanosensor. Int. J. Electrochem. Sci. 10, 303–316 (2015)Google Scholar
- 6.G. Kh, S. Bonyadi, An electrochemical sensor based on reducedgraphene oxide decorated with polypyrrolenanofibers and zinc oxide–copper oxide p–n junction heterostructures for the simultaneous voltammetric determination of ascorbic acid, dopamine, paracetamol, and tryptophan. New J. Chem. 42, 8512–8523 (2018)CrossRefGoogle Scholar
- 12.N. Butwong, L. Zhou, W. Ng-eontae, R. Burakham, E. Moore, S. Srijaranai, J.H.T. Luong, J.D. Glennon, A sensitive nonenzymatic hydrogen peroxide sensor using cadmium oxide nanoparticles/multiwall carbon nanotube modified glassy carbon electrode. J. Electroanal. Chem. 717–718, 41–46 (2014)CrossRefGoogle Scholar
- 27.M. Dekker, in Laboratory Techniques in Electroanalytical Chemistry, ed. by P.T. Kissinger, W.R. Heineman (New York: Marcel Dekker, 1984), p. 82Google Scholar
- 35.F. Öztürk, P.E. Erden, C. Kaçar, E. Kiliç, Amperometric biosensor for xanthine determination based on Fe3O4 nanoparticles. Acta Chim. Slov. 61, 19–26 (2014)Google Scholar