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Effect of deproteinization methods on the antioxidant activity of polysaccharides extracted from Lentinus edodes stipe

  • Shunfeng LiEmail author
  • Anjian Wang
  • Lina Liu
  • Guangrui Tian
  • Fangfang Xu
Original Paper
  • 8 Downloads

Abstract

Removal of protein from plant polysaccharide extract is often regarded as essential step for antioxidant activity evaluation. The aim of this study was to compare two different methods (neutral protease and Sevag) for efficient removal of protein from Lentinus edodes stipe polysaccharide extract. Orthogonal test was used to optimize the parameters of neutral protease deproteinization effects on polysaccharide extracted from Lentinus edodes stipe. Furthermore, antioxidant activity was also investigated by the two deproteinization methods. Results showed that the optimum parameters of deproteinization by neutral protease were pH of 6, temperature of 60 °C, time of 1 h, and enzyme amount 0.2%. Under these conditions, the polysaccharide retention rate was 92.29%, and the deproteinization rate was 84.30%. This result was higher than that of Sevag, which deproteinization rate and polysaccharide retention rate were 55.43% and 82.29%, respectively. The antioxidant activity of polysaccharide declined after the polysaccharide deproteinized for both techniques, however, the polysaccharide deproteinized by Sevag showed the lowest antioxidant activity. Therefore, neutral protease could be used for deproteinization of polysaccharide extracted from Lentinus edodes stipe.

Keywords

Lentinus edodes stipe Polysaccharide Deproteinization Neutral protease Sevag Antioxidant activity 

Notes

Acknowledgements

This work was financially supported by the Special Fund for Independent Innovation Research (Grant No. YUCAIKE [2018]9) established by Henan Academy of Agricultural Sciences, Scientific and Technological Project of Henan Province (Grant Nos. 182102110276, 172102110085), China.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Agro-Products ProcessingHenan Academy of Agricultural SciencesZhengzhouPeople’s Republic of China

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