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Cytotoxic and genotoxic activity of protein isolate of ayocote beans and anticancer activity of their protein fractions

  • Gerardo Teniente-Martínez
  • Aurea Bernardino-Nicanor
  • Raquel Cariño-Cortés
  • María del Carmen Valadez-Vega
  • José Luis Montañez-Soto
  • Gerardo Acosta-García
  • Leopoldo González-CruzEmail author
Original Paper
  • 26 Downloads

Abstract

We evaluated the cytotoxic and genotoxic effects of protein isolates of ayocote beans on adult male CD1+ mice and the anticancer activity of their protein fractions using SiHa Cells. The mice that received protein isolate showed a Polychromatic Erythrocytes (PCE) increase at 48 h; a significant PCE decrease was observed in mice administered the protein isolate-Daunorubicin (isolate-DAU) mix. A micronucleated erythrocytes (MNE) increase was observed in mice that received the mix in all times tested. All protein fractions of ayocote beans (black and purple) showed inhibition against SiHa cells proliferation at doses of 3000 or 5000 µg/mL. The maximal inhibitory concentration (IC50) of each protein fraction was reached at a concentration of 5000 µg/mL. A synergistic effect of isolate-DAU was observed in the in vivo test; the effect of the protein fractions on SiHa cells proliferation depended of the ayocote bean variety used.

Keywords

Ayocote bean Cytotoxic Genotoxic Protein fractions SiHa cells 

Notes

Acknowledgements

The authors would like to acknowledge the Autonomous University of Hidalgo State for technical services during this study.

Funding

This work was supported by the National Technology of Mexico [grant number 5938.16-P.A-P and grant number 6244.17-P].

Compliance with ethical standards

Conflict of interest

All the authors declare that there are no conflicts of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Gerardo Teniente-Martínez
    • 1
    • 2
  • Aurea Bernardino-Nicanor
    • 2
  • Raquel Cariño-Cortés
    • 3
  • María del Carmen Valadez-Vega
    • 3
  • José Luis Montañez-Soto
    • 4
  • Gerardo Acosta-García
    • 2
  • Leopoldo González-Cruz
    • 2
    Email author return OK on get
  1. 1.Doctorado en Ciencias en Ingeniería Bioquímica, Tecnológico Nacional de México-CelayaCelayaMéxico
  2. 2.Tecnológico Nacional de México-CelayaCelayaMéxico
  3. 3.Instituto de Ciencias de la SaludUniversidad Autónoma del Estado de Hidalgo, Ex-Hacienda de la Concepción, TilcuautlaPachuca de SotoMexico
  4. 4.Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional del Instituto Politécnico NacionalUnidad MichoacánJiquilpanMéxico

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