Journal of Food Measurement and Characterization

, Volume 7, Issue 3, pp 122–128 | Cite as

Concentration profile of selected polycyclic aromatic hydrocarbon (PAH) fractions in some processed meat and meat products

  • Olatunde S. OlatunjiEmail author
  • Beatrice O. Opeolu
  • Olalekan S. Fatoki
  • Bhekumusa J. Ximba
Original Paper


This study investigates the concentration profiles of selected polycyclic aromatic hydrocarbon (PAH) fractions in selected processed meats, in order to evaluate their dietary and health implications. Smoked, grilled and boiled meat products were bought from different locations in Cape Town and Cape Town environs. PAHs were extracted from each meat sample according to standard methods. The concentrations of benzo[k]fluoranthene (BkP), benzo[a]pyrene (BaP), indeno[1, 2, 3-cd]pyrene (IP), and benzo[g, h, i]perylene(BghiP) in the processed meat extracts were determined using a gas chromatograph coupled with flame ionization detector. Total PAH concentrations in smoked, grilled and boiled chicken fillets, pork, and beef stripes were ranged 2.79, 0.99, 2.33 μg/kg; 19.11, 11.17, 15.04 μg/kg; and 14.84, 9.29, 7.20 μg/kg respectively. There were significant differences (p > 0.05) in the concentration levels of PAHs detected in different types, with the highest levels observed in smoked pork meat. The concentration of BkP, BaP, IP and BghiP detected in the various meat samples were below the EU and WHO dietary exposure limit.


Polycyclic aromatic hydrocarbons Processed meat and meat products Concentration profile Separation Dietary exposure limit 



Appreciation goes to Prof Van de Watt for providing a large proportion of the funding for this project. We also wish to thank immensely thank Ms Zandile Mthembu, Ms Jilian Fennesy-Yong and Ms Khanyisa Nohako for their support and assistant during the study.


  1. 1.
    A.C. Marques, M.R. Marostica (Jr.), G.M. Pastore, Some nutritional, technological and environmental advances in the use of enzymes in meat products. Enzyme Research, (Article ID 480923) (2010)Google Scholar
  2. 2.
    J.D. Higgs, The changing nature of red meat: 20 years of improving nutritional quality. Trends Food Sci Technol. 11, 85–95 (2000)CrossRefGoogle Scholar
  3. 3.
    E.A. Gomaa, J.I. Gray, S. Rabie, C. Lopez-Bote, A.M. Booren, Polycyclic aromatic hydrocarbons in smoked food products and commercial liquid smoke flavouring. Food Addi Contam 10(5), 503–521 (1993)CrossRefGoogle Scholar
  4. 4.
    M.N.P. Linda, D. Carboo, O.P. Yeboah, J.W. Quasie, O.M. Gorleku, A. Darko, Characterization of polycyclic aromatic hydrocarbons (PAHs) present in smoked fish from Ghana. Adv J Sci Technol 3(5), 332–338 (2011)Google Scholar
  5. 5.
    D.H. Phillips, Polycyclic aromatic hydrocarbons in the diets. Mutat Res 443(1–2), 139–147 (1999)Google Scholar
  6. 6.
    A. Ramesh, S.A. Walker, D.B. Hood, M.D. Guillen, H. Schneider, E.H. Weyand, Bioavailability and risk assessment of orally ingested polycyclic aromatic hydrocarbons. Int J Toxicol 23, 301–333 (2004)CrossRefGoogle Scholar
  7. 7.
    G. Grimmer, F. Pott, Occurrence of PAH, in Environmental carcinogens. Polycyclic aromatic hydrocarbons, ed. by G. Grimmer (CRC Press Inc, Boca Raton, 1983), pp. 61–128Google Scholar
  8. 8.
    E. Wegrzyn, S. Grzes′kiewicz, W. Poplawska, B.K. Glod, Modified analytical method for polycyclic aromatic hydrocarbons, using CEC for sample preparation and RP-HPLC with fluorescence detection: application to different food samples. Acta Chromatographica 17, 233–249 (2007)Google Scholar
  9. 9.
    H. Reiner, Analysis of smoke and smoked foods. Pure Appl Chem 49, 1655–1666 (1977)CrossRefGoogle Scholar
  10. 10.
    W. Fiddler, R.C. Doerr, A.E. Wasserman, Composition of an ether-soluble fraction of a liquid smoke solution. J Agric Food Chem 18, 310–312 (1975)CrossRefGoogle Scholar
  11. 11.
    J.M. Lorenzo, L. Purrinos, M.C. Garcia Fontan, D. Franco, Polycyclic aromatic hydrocarbons (PAHs) in two Spanish traditional smoked sausage varieties: “Androlla” and “Botillo”. Meat Sci 86, 660–664 (2010)CrossRefGoogle Scholar
  12. 12.
    N.Y. Chen, T.Y. Yan, M2 Forming—a process for aromatization of light hydrocarbons. Ind Eng Chem Process Des Dev 25, 151–155 (1986)CrossRefGoogle Scholar
  13. 13.
    W. Lijinsky, P. Shubik, Benzo[a]pyrene and other polynuclear aromatic hydrocarbons in charcoal-broiled meat. Science 145, 53–55 (1964)CrossRefGoogle Scholar
  14. 14.
    K. Fretheim, Polycyclic aromatic hydrocarbons in grilled meat products—a review. Food Chem 10, 129–139 (1983)CrossRefGoogle Scholar
  15. 15.
    O.A. Afolabi, E.A. Adesulu, O.L. Oke, Polynuclear aromatic hydrocarbons in some Nigerian preserved freshwater fish species. J Agric Food Chem 31, 1083–1090 (1983)CrossRefGoogle Scholar
  16. 16.
    D.O. Alonge, Carcinogenic polycyclic aromatic hydrocarbons (PAH) determined in Nigerian Kundi (smoked dried meat). J Sci Food Agric 43, 167–173 (1988)CrossRefGoogle Scholar
  17. 17.
    S. Moret, G. Purcaro, L.S. Conte, Polycyclic aromatic hydrocarbons in vegetable oils from canned foods. Eur J Lipid Sci Technol 107(7&8), 488–496 (2010)Google Scholar
  18. 18.
    H. Soderstrom, J. Hajslova, V. Kocourek, B. Siemund, A. Kocan, M.W. Obiedzinski, M. Tysklind, P.-A. Bergqvist, PAHs and nitrated PAHs in air of five European countries determined using SPMDs as passive samplers. Atmos Environ 39, 1627–1640 (2005)Google Scholar
  19. 19.
    United States Environmental Protection Agency (US EPA, Environmental protection agency (EPA) of the United States of America: compendium method TO-13A (EPA, Cincinnati, 1999)Google Scholar
  20. 20.
    International Agency for Cancer Research IARC (2002). index.php)
  21. 21.
    Scientific Committee on Food (SCF), Polycyclic aromatic hydrocarbons—Occurrence in foods, dietary exposure and health effects. Scientific Committee on Food.SCF/CS/CNTM/PAH/29 ADD1 Final (2010)Google Scholar
  22. 22.
    EFSA, Polycyclic aromatic hydrocarbons in food. Scientific opinion of the panel on contaminants in the food chain (Question N° EFSA-Q-2007-136). EFSA J. 724, 1–114 (2008)Google Scholar
  23. 23.
    D.S. Silvester, Determination of 3, 4-benzopyrene and benzoanthracene in phenolic smoke concentrate. J Food Technol 15, 413–420 (1980)CrossRefGoogle Scholar
  24. 24.
    P. Simko, S. Gergely, J. Karovicova, M. Drdak, J. Knezo, Influence of cooking on benzo[a]pyrene content in smoked saugages. Meat Sci 34, 301–309 (1993)CrossRefGoogle Scholar
  25. 25.
    B. Janoszka, Analytical methods: HPLC-fluorescence analysis of polycyclic aromatic hydrocarbons (PAHs) in pork meat and its gravy fried without additives and in the presence of onion and garlic. Food Chem 126, 1344–1353 (2011)CrossRefGoogle Scholar
  26. 26.
    L.C. Dolan, R.A. Matulka, G.A. Burdock, A review: naturally occurring food toxins. Toxins 2, 2289–2332 (2010)CrossRefGoogle Scholar
  27. 27.
    B. Janoszka, L. Warzecha, U. Blaszczyk, D. Bodzek, Organic compounds formed in thermally treated high-protein food. Part I: Polycyclic aromatic hydrocarbons. Acta Chromatographica 14, 115–128 (2004)Google Scholar
  28. 28.
    B.K. Larsson, Polycyclic aromatic hydrocarbons in Swedish foods: aspects on analysis, occurrence and intake. Doctoral thesis (Swedish University Sciences, Uppsala, 1986)Google Scholar
  29. 29.
    L. To′th, K. Potthast, Chemical aspects of the smoking of meat and meat products. Adv Food Res 29, 87–158 (1984)CrossRefGoogle Scholar
  30. 30.
    W. Lijinsky, A.E. Ross, Production of carcinogenic polynuclear hydrocarbons in the cooking of food. Food Cosmet Toxicol 5, 343–347 (1967)CrossRefGoogle Scholar
  31. 31.
    L. To′th, W. Blaas, The effect of smoking technology on the content of carcinogenic hydrocarbons in smoked meat products. II. Effect of smoldering temperature of wood and of cooling, washing and filtering of smoke. Fleischwirtschaft 52, 1419–1422 (1972)Google Scholar
  32. 32.
    M.E. Doremire, G.E. Harmon, D.E. Pratt, 3,4-Benzopyrene in charcoal grilled meats. J Food Sci 44, 622–623 (1979)CrossRefGoogle Scholar
  33. 33.
    M.N. Moore, Cytochemical responses of the lysosomal system and NADPH-ferrihemoprotein reductase in molluscan digestive cells to environmental and experimental exposure to xenobiotics. Mar Ecol Prog Ser 46, 81–89 (1988)CrossRefGoogle Scholar
  34. 34.
    J. Santodonato, D. Basu, P.H. Howard, Multimedia human exposure and carcinogenic risk assessment for environmental PAH, in Polynuclear aromatic hydrocarbons: chemistry and biological effects, ed. by A. Bjorseth, A.J. Dennis (Battelle Press, Columbus, 1980), pp. 435–454Google Scholar
  35. 35.
    J. Santodonato, P. Howard, D. Basu, in Health and ecological assessment of polynuclear aromatic hydrocarbons, ed. by S.D. Lee, L. Grant (Pathotox Publishers, Park Forest South, 1981), p. 364Google Scholar
  36. 36.
    FAO/WHO, Joint FAO/WHO Expert Committee on Food Additives (JECFA). Sixty-fourth meeting (64/SC) (2010). Section 2.6, from Accessed on 21 July 2012

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Olatunde S. Olatunji
    • 1
    Email author
  • Beatrice O. Opeolu
    • 2
  • Olalekan S. Fatoki
    • 1
  • Bhekumusa J. Ximba
    • 1
  1. 1.Department of Chemistry, Faculty of Applied SciencesCape Peninsula University of TechnologyCape TownSouth Africa
  2. 2.Department of Environmental and Occupational Studies, Faculty of Applied SciencesCape Peninsula University of TechnologyCape TownSouth Africa

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