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Frontiers of Medicine

, Volume 9, Issue 3, pp 331–343 | Cite as

MicroRNA-142-3p and microRNA-142-5p are downregulated in hepatocellular carcinoma and exhibit synergistic effects on cell motility

  • Felice Ho-Ching Tsang
  • Sandy Leung-Kuen Au
  • Lai Wei
  • Dorothy Ngo-Yin Fan
  • Joyce Man-Fong Lee
  • Carmen Chak-Lui Wong
  • Irene Oi-Lin Ng
  • Chun-Ming Wong
Research Article

Abstract

MicroRNAs (miRNAs), an important class of small non-coding RNAs, regulate gene expression at the post-transcriptional level. miRNAs are involved in a wide range of biological processes and implicated in different diseases, including cancers. In this study, miRNA profiling and qRT-PCR validation revealed that miR-142-3p and miR-142-5p were significantly downregulated in hepatocellular carcinoma (HCC) and their expression levels decreased as the disease progressed. The ectopic expression of miR-142 significantly reduced HCC cell migration and invasion. Overexpression of either miR-142-3p or miR-142-5p suppressed HCC cell migration, and overexpression of both synergistically inhibited cell migration, which indicated that miR-142-3p and miR-142-5p may cooperatively regulate cell movement. miR-142-3p and miR-142-5p, which are mature miRNAs derived from the 3'- and 5'-strands of the precursor miR-142, target distinct pools of genes because of their different seed sequences. Pathway enrichment analysis showed a strong association of the putative gene targets of miR-142-3p and miR-142-5p with several cell motility-associated pathways, including those regulating actin cytoskeleton, adherens junctions, and focal adhesion. Importantly, a number of the putative gene targets were also significantly upregulated in human HCC cells. Moreover, overexpression of miR-142 significantly abrogated stress fiber formation in HCC cells and led to cell shrinkage. This study shows that mature miR-142 pairs collaboratively regulate different components of distinct signaling cascades and therefore affects the motility of HCC cells.

Keywords

hepatocellular carcinoma microRNA metastasis cytoskeletal reorganization 

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Copyright information

© Higher Education Press and Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Felice Ho-Ching Tsang
    • 1
  • Sandy Leung-Kuen Au
    • 1
  • Lai Wei
    • 1
  • Dorothy Ngo-Yin Fan
    • 1
  • Joyce Man-Fong Lee
    • 1
  • Carmen Chak-Lui Wong
    • 1
  • Irene Oi-Lin Ng
    • 1
  • Chun-Ming Wong
    • 1
  1. 1.State Key Laboratory for Liver Research and Department of Pathology, Li Ka Shing Faculty of MedicineThe University of Hong KongHong Kong SARChina

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