Frontiers of Medicine in China

, Volume 3, Issue 1, pp 49–56 | Cite as

Gene expression disparity in giant cell tumor of bone

  • Xiaohua Pan
  • Shuhua Yang
  • Deming Xiao
  • Yong Dai
  • Lili Ren
Research Article
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Accepted:
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Abstract

The aim of this paper was to study the differential gene expression of giant cell tumor of bone (GCTB) by gene chip technology. Total RNA of 8 fresh GCTB specimens (Jaffe I:6 cases, II:1 case, III:1 case; Campanacci I:6 cases, II:1 case, III:1 case; Enneking Staging G0T1−2M0, 5 cases, G1T1−2M0: 2 cases, G1T2M0: 1 case) and 4 normal bony callus specimens (the control group) were extracted and purified to get mRNA and then reverse transcribed to complementary DNA, respectively. Microarray screening with a set of 8064 human cDNA genes was conducted to analyze the difference among the samples and the control. The hybridization signals were scanned. The gene expression disparity between the GCTB samples and normal bony callus was significantly different (P < 0.01), and the disparity of over 5-fold was found in 47 genes in the GCTB specimens, with 25 genes up-regulated and 22 down-regulated including the extracellular matrix and transforming-related genes, oncogene and its homolog genes, cytokine and its receptor genes. Specific gene spectrum associated with GCTB can be identified by cDNA microarray, which will be the foundation of progressive etiology elucidation, diagnosis and treatment of GCTB.

Keywords

giant cell tumor of bone gene microarray, cDNA 
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Copyright information

© Higher Education Press and Springer-Verlag GmbH 2009

Authors and Affiliations

  • Xiaohua Pan
    • 1
    • 2
  • Shuhua Yang
    • 1
  • Deming Xiao
    • 2
  • Yong Dai
    • 3
  • Lili Ren
    • 3
  1. 1.Department of Orthopaedics, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.Department of Orthopaedics, Shenzhen People’s HospitalThe Second Medical College of Jinan UniversityShenzhenChina
  3. 3.Clinical Research Center, Shenzhen People’s HospitalThe Second Hospital Medical College of Jinan UniversityShenzhenChina

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