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The potential of arbuscular mycorrhizal fungi to conserve Kalappia celebica, an endangered endemic legume on gold mine tailings in Sulawesi, Indonesia

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Abstract

Kalapi (Kalappia celebica) is an endemic legume of Sulawesi and has been included in the endangered category since the early 1980s. Conservation of the species is possible through ex situ culture techniques. Arbuscular mycorrhizal fungi (AMF) can accelerate plant growth which in turn supports the conservation of endangered species. This study aimed to assess the efficacy of local AMF to accelerate the growth of kalapi and increase nutrient uptake in kalapi grown in gold mine tailing media. There were three AMF treatments, Glomus claroideum, Glomus coronatum, and a mixture of both, plus the control. Each treatment was replicated three times, each consisting of five plants. The results show that the highest AMF colony was obtained by kalapi seedlings inoculated with Glomus coronatum and the mixture of AMF. The range of mycorrhizae inoculation effect values was 59.7–71.3%. AMF inoculation increased growth and dry weight of 4-month-old seedlings compared to controls. Dry and total weights of kalapi inoculated with G. coronatum were significantly different from those inoculated with the AMF mixture. However, they are not significantly different from kalapi inoculated with G. claroideum. The results also show that AMF increased nitrogen and phosphorous uptake by the roots, as well as nitrogen, phosphorous, potassium, manganese and iron by the shoots. All AMF treatments decreased potassium uptake in the roots, except in kalapi inoculated with G. coronatum. The AMF mixture decreased iron contents the roots by 15%. AMF can be developed into biofertilizer to support the conservation of kalapi in tropical Indonesia.

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References

  1. Abbott LK, Johnson NC (2017) Introduction: perspectives on mycorrhizas and soil fertility. In: Johnson NC, Gehring C, Jansa J (eds) Mycorrhizal mediation of soil fertility, structure, and carbon storage. Academic Press, New York, pp 93–105

  2. Arif A, Tuheteru FD, Kandari AM, Husna Mekuo IS, Masnun (2016) Status and culture of arbuscular mycorrhizal fungi isolated from rhizosphere of endemic and endangered species of Kalapi (Kalappia celebica Kosterm). Eur J Sus Dev 5(4):395–402

  3. Augé RM (2004) Arbuscular mycorrhizae and soil/plant water relations. Can J Soil Sci 84:373–381

  4. Barua A, Gupta SD, Mridha MAU, Bhuiyan MK (2010) Effect of arbuscular mycorrhizal fungi on growth of Gmelina arborea in arsenic-contaminated soil. J For Res 21(4):423–432

  5. Bothe H, Turnau K, Regvar M (2010) The potential role of arbuscular mycorrhizal fungi in protecting endangered plants and habitats [review]. Mycorrhiza 20:445–457

  6. Brown PH (2006) Nickel. In: Barker AV, Pilbeam DJ (eds) Handbook of plant nutrition. CRC Taylor & Francis, New York, pp 395–410

  7. Brundrett M, Bougher N, Deu B, Grove T, Majalaczuk (1996) Working with mycorrhizas in forestry and agriculture. Australian Centre for International Agriculture Research, Canberra

  8. Carter MR (1993) Soil sampling and methods of analysis Boca Raton. Lewis Publishers, USA

  9. Choosa-Nga P, Sangwanit U, Kaewgrajang T (2019) The arbuscular mycorrhizal fungi’s diversity in fabaceous trees species of Northeastern Thailand. Biodiversitas 20(2):405–412

  10. Duryea ML, Brown GN (1984) Seedling physiology and reforestation success. In: Proceeding of the physiology working group Technical Session. Dr. W. Juck Publishers, Boston

  11. Edwards DP, Socolar JB, Mills SC, Burivaloka Z, Koh LP, Wilcove DS (2019) Conservation of tropical forests in the anthropocene. Curr Biol Rev 29:R1008–R1020

  12. Evelin H, Kapoor R, Giri B (2009) Arbuscular mycorrhizal fungi in alleviation of salt stress: a review. Ann Bot 104:1263–1280

  13. Fiqri A, Utomo WH, Handayanto E (2016) Effect of arbuscular mycorrhizal fungi on the potential of three wild plant species for phytoextraction of mercury from small-scale gold mine tailings. J Degrade Min Land Manag 3(3):551–558

  14. Fuchs B, Haselwandter K (2008) Arbuscular mycorrhiza of endangered plant species: potential impacts on restoration strategies. In: Varma A (ed) Mycorrhiza. Springer, Berlin

  15. González-Chávez MC, Carrillo-Gonzales R, Wright SF, Nichols KA (2004) The role glomalin, a protein produced by mycorrhizal fungi, in sequestering potentially toxic elements. Environ Pollut 130:317–323

  16. Habte M, Manjunath A (1991) Categories of vesicular–arbuscular mycorrhizal dependency of host species. Mycorrhiza 1:3–12

  17. He WY, Fan XX, Zhou ZX, Zhang HH, Gao X, Song FQ, Geng G (2019) The effect of Rhizophagus irregularis on salt stress tolerance of Elaeagnus angustifolia roots. J For Res 5:1–10. https://doi.org/10.1007/s11676-019-01053-1

  18. Husna Budi RSW, Mansur I, Kusmana C (2015) Growth response of kayu kuku (Pericopsis mooniana (Thw.) Thw) seedling to indigenous arbuscular mycorrhizal fungi inoculation. Jurnal Pemuliaan Tanaman Hutan 9(3):131–148

  19. Husna, Budi RSW, Mansur I, Kusmana C (2016) Growth and nutrient status of kayu kuku (Pericopsis mooniana Thw.) with micorrhiza in soil media of nickel post mining. Pak J Biol Sci 19:158–170

  20. Husna, Tuheteru FD, Arif A (2017a) Arbuscular mycorrhizal fungi and plant growth on serpentine soils. In: Wu QS (ed) Arbsucular mycorrhizas and stress tolerance of plants. Springer, Singapore, pp 293–303

  21. Husna, Tuheteru FD, Wigati E (2017b) Growth response and dependency of endangered nedum tree species (Pericopsis mooniana) affected by indigenous arbuscular mycorrhizal fungi inoculation. Nusant Biosci 9(1):57–61

  22. Husna, Tuheteru FD, Arif A (2018) Arbuscular mycorrhizal fungi symbiosis and conservation of endangered tropical legume trees. In: Giri B et al (eds) Root biology, soil biology 52. Springer, Germany, pp 465–486

  23. Husna, Mansur I, Budi RSW, Tuheteru FD, Arif A, Tuheteru EJ, Albasri (2019) Effects of arbuscular mycorrhizal fungi and organic material on growth and nutrient uptake by Pericopsis mooniana in coal mine. Asian J Plant Sci 18(3):101–109

  24. IUCN (1994) IUCN Red List Categories. Prepared by the IUCN Species Survival Commission. IUCN, Gland Switzerland

  25. Joner EJ, Leyval C (1997) Uptake of 109Cd by roots and hiphae of a Glomus mosseae/Trifolium subterraneum Mycorrhiza from soil amended with high and low concentration of cadmium. New Phytol 135:353–360

  26. Kafkas S, Ortas I (2009) Various mycorrhizal fungi enhance dry weights, P and Zn uptake of four Pistacia species. J Plant Nutr 32:146–159

  27. Lambers H, Chapin FS III, Pons TL (2008) Plant physiology ecology, 2nd edn. Springer, New York

  28. Liam AT, Arif A, Clark RP, Girmansyah D, Kintamani E, Prychid CJ, Pujirahayu N, Rosmarlinansiah, Brearly FQ, Utteridge TMA, Lewis GP (2019) An enigmatic genus on an enigmatic island: the re-discovery of Kalappia on Sulawesi. Ecology 100(11):e02793

  29. Madejon E, Doronila AI, Madejon P, Baker AJM, Woodrow IE (2012) Biosolids, mycorrhizal fungi and eucalypts for phytostabilization of arsenical sulphidic mine tailings. Agrofor Syst 84(3):389–399

  30. Miransari M (2010) Contribution of arbuscular mycorrhizal symbiosis to plant growth under different types of soil stress. Plant Biol 12:563–569

  31. Miransari M (2017) Arbuscular mycorrhizal fungi and heavy metal tolerance in plants. In: Wu QS (ed) Arbuscular mycorrhizas and stress tolerance of plants. Springer, Singapore, pp 147–162

  32. Muin A (2003) Pertumbuhan anakan Ramin (Gonystylus bancanus (Miq.) Kurz)) dengan inokulasi cendawan mikoriza arbuskula (CMA) pada berbagai intensitas cahaya dan dosis fosfat alam [dissertation]. Program Pascasarjana Institut Pertanian Bogor

  33. Orłowska E, Jurkiewicz A, Anielska T, Godzik B, Turnau K (2005) Influence of different arbuscular mycorrhiza fungal (AMF) strains on heavy metal uptake by Plantago lanceolata (Plantaginaceae). Pol Bot Stud 19:65–72

  34. Orłowska E, Orłowski D, Mesjasz-Przybyłowicz J, Turnau K (2011) Role of ycorrhizal colonization in plant establishment on an alkaline gold mine tailing. Int J Phytoremediat 13:185–205

  35. Panwar J, Tarafdar JC (2006) Distribution of three endangered medicinal plant species and their colonization with arbuscular mycorrhizal fungi. J Arid Environ 65:337–350

  36. Peraturan Menteri Kehutanan (the Minister of Forestry Regulation) No. P.57/Menhut-II/2008 (2008) tentang Arahan Strategi Konservasi Spesies Nasional 2008–2018

  37. Santoso E, Gunawan AW, Turjaman M (2007) Kolonisasi cendawan mikoriza arbuskula pada bibit tanaman penghasil gaharu Aquilaria microcarpa Baill. Jurnal Penelitian Hutan dan Konservasi ALam, IV 5:499–509

  38. Sharma D, Rupan K, Bhatnagar AK (2008) Arbuscular mycorrhizal (AM) technology for the conservation of Curculigo orchioides Gaertn.: an endangered medicinal herb. World J Microbiol Biotechnol 24:395–400

  39. Smith SE, Read DJ (2008) Mycorrhizal symbiosis, 3rd edn. Academic Press, London

  40. Sosef MSM, Hong LT, Prawirohatmodjo S (1998) Timber trees: lesser-known timbers No 5 (3). Prosea, Bogor

  41. Tuheteru FD, Kusmana C, Mansur I, Iskandar (2015) Response of lonkida (Nauclea orientalis L.) towards mycorrhizal inoculum in waterlogged condition. Biotropia 22(1):61–71

  42. Turjaman M, Santosa E, Sumarna Y (2006a) Arbuscular mycorrhizal fungi increased early growth of gaharu wood species Aquilaria malaccensis and A. crasna under greenhouse conditions. J For Res 3(2):139–148

  43. Turjaman M, Tamai Y, Santoso E, Osaki M, Tawaraya K (2006b) Arbuscular mycorrhizal fungi incresead early growth of two nontimber forest product species Dyera polyphylla and Aquilaria filaria under greenhouse conditions. Mycorrhiza 16:459–464

  44. UNEP-WCMC (2007) Strategies for the sustainable use and management of timber tree species subject to international trade: South East Asia. Cambridge

  45. Wang FY (2017) Occurrence of arbuscular mycorrhizal fungi in mining-impacted sites and their contribution to ecological restoration: Mechanisms and applications. Crit Rev Environ Sci Technol 47:1901–1957

  46. Wang FY, Lin XG, Yin R (2005) Heavy metal uptake by arbuscular mycorrhizas of Elsholtzia splendens and the potential for phytoremediation of contaminated soil. Plant Soil 269:225–232

  47. Whitmore TC, Tantra IGM, Sutisna U (1989) Tree flora of Indonesia check list for Sulawesi. Forest Research and Development Centre, Forestry of Departemen, Bogor

  48. Whitten AJ, Mustafa M, Henderson GS (1987) Ekologi Sulawesi. Gajah Mada University Press, Yogyakarta

  49. Wulandari D, Saridi ChengWG, Tawaraya K (2016) Arbuscular mycorrhizal fungal inoculation improves Albizia saman and Paraserianthes falcataria growth in post-opencast coal mine in East Kalimantan, Indonesia. For Ecol Manag 376:67–73

  50. Zhu XC, Song FB, Liu SQ, Liu TD, Zhou X (2012) Arbsucular mycorrhizae improves photosynthesis and water status of Zea mays L. under drought stress. Plant Soil Environ 58(4):186–191

  51. Zubek S, Turnau K, Tsimilli-Michael M, Strasser RJ (2009) Response of endangered plant species to inoculation with arbuscular mycorrhizal fungi and soil bacteria. Mycorrhiza 19:113–123

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Correspondence to Husna.

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Project funding: The work was supported by the funding support (No. 171.SP2H/LT/DRPM/2019) from Directorate General of Resource, Science, Technology, and Higher Education, Ministry of Research, and Technology of the Republic of Indonesia.

The online version is available at http://www.springerlink.com

Corresponding editor: Tao Xu.

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Husna, Tuheteru, F.D. & Arif, A. The potential of arbuscular mycorrhizal fungi to conserve Kalappia celebica, an endangered endemic legume on gold mine tailings in Sulawesi, Indonesia. J. For. Res. (2020) doi:10.1007/s11676-020-01097-8

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Keywords

  • Kalappia celebica
  • Glomeromycota
  • Glomus coronatum
  • Gold mine tailings
  • Southeast Sulawesi