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Overexpression of CfPIP1-1, CfPIP1-2, and CfPIP1-4 genes of Catalpa fargesii in transgenic Arabidopsis thaliana under drought stress

  • Wenjun Ma
  • Yao Xiao
  • Yuan Li
  • Pan Hu
  • Zhi Wang
  • Guijuan Yang
  • Junhui WangEmail author
Original Paper
  • 8 Downloads

Abstract

Catalpa fargesii is an important economic tree species used for furniture and timber production because of its high density and hardness. Its survival and growth are severely affected and primarily limited by drought stress. Thus, to better understand the mechanism of drought resistance in C. fargesii, we used qRT-PCR to reveal significantly different expression of three plasma membrane intrinsic protein genes: CfPIP1-1, CfPIP1-2 and CfPIP1-4. We then cloned their full-length cDNA sequences and characterized the encoded proteins. We analyzed the genes phylogenetically and predicted conserved motifs, domains, and secondary and tertiary structures. To verify the function of the CfPIP1 genes further, we ectopically expressed CfPIP1 transgenes in Arabidopsis thaliana. The results showed that CfPIP1-1, CfPIP1-2 and CfPIP1-4 had several characteristics of aquaporins. The transgenic plants grew better than the WT plants did under drought stress, and overexpression of the CfPIP1 genes increased the plant water content and resistance to drought. Thus, CfPIP1-1, CfPIP1-2 and CfPIP1-4 of C. fargesii play key roles in regulating the intracellular and extracellular water balance and in mediating the plant response to drought.

Keywords

Catalpa fargesii Drought tolerance PIP1 Aquaporin Heterologous transformation 

Notes

Supplementary material

11676_2019_1082_MOESM1_ESM.docx (26 kb)
Supplementary material 1 (DOCX 25 kb)

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Copyright information

© Northeast Forestry University 2020

Authors and Affiliations

  • Wenjun Ma
    • 1
  • Yao Xiao
    • 1
  • Yuan Li
    • 1
    • 3
  • Pan Hu
    • 1
    • 2
  • Zhi Wang
    • 1
  • Guijuan Yang
    • 1
  • Junhui Wang
    • 1
    Email author
  1. 1.State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation, National Forestry and Grassland Administration, National Forest Genetic Resource Platform, Research Institute of ForestryChinese Academy of ForestryBeijingPeople’s Republic of China
  2. 2.Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijingPeople’s Republic of China
  3. 3.Central South University of Forestry and TechnologyChangshaPeople’s Republic of China

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