Simple phenols in tropical woods determined by UHPLC-PDA and their antioxidant capacities: an experimental design for Randall extraction using environmentally friendly solvents

  • Marie Bartakova
  • Eva DvorackovaEmail author
  • Lucie Chromcova
  • Petr Hrdlicka
Original Paper


A comprehensive experimental design was developed to determine suitable conditions (volume, percentage of solvent, extraction temperature) for the ethanol extraction of phenolic compounds from ten tropical woody plants. Wenge, angelim vermelho, zebrano, merbau, tigerwood, angelim pedra, jatoba, angelim amargoso, massaranduba, and doussie woods were used in experiments. The effects of three independent variables and their interactions on the yields of cinnamic and benzoic acid derivates were analyzed using UHPLC-PDA. The most significant parameters were found to be solvent percentage, extraction volume, and extraction temperature. Optimal conditions for the extraction of phenolic compound contents were an 80 °C extraction temperature, a 30 mL extraction volume, and the use of pure water as the extraction solvent. The tested species of woods contained mainly cinnamic acid derivates. Water extracts after hydrolysis contained greater amounts of cinnamic acid derivates than those extracts from non-hydrolyzed material. The total phenolics content was highest in water extracts of tigerwood, wenge and merbau; however, the extract of merbau wood was a stronger radical scavenger in ABTS assays: 34.11 ± 0.02 mM Trolox equivalents per g of dry extract. The main compounds possessing antioxidant activity in the extracts of vermelho wood assessed by UHPLC analysis were hydroxycinnamic acids.


Phenolic compounds Tropical woods Ultrahigh-performance liquid chromatography Antioxidant capacity 



Antioxidant capacity




Dry weight






Photodiode array detector


Solid-phase extraction


Total equivalent antioxidant capacities


Total polyphenol content


Ultrahigh-performance liquid chromatography



We would like to thank the Faculty of Forestry and Wood Technology, Department of Wood Science for providing wood samples. The authors would also like to thank the companies MAGNUM Parket a.s. and ToTEM s.r.o. for the supply of materials used in the experiment. We would like to thank Mr. Matthew Nicholls for manuscript improvement and English correction.


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Copyright information

© Northeast Forestry University 2019

Authors and Affiliations

  • Marie Bartakova
    • 1
  • Eva Dvorackova
    • 1
    Email author
  • Lucie Chromcova
    • 1
  • Petr Hrdlicka
    • 1
  1. 1.Department of Chemistry and BiochemistryMendel University in BrnoBrnoCzech Republic

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