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Journal of Forestry Research

, Volume 30, Issue 3, pp 1105–1113 | Cite as

Matrine inhibits mycelia growth of Botryosphaeria dothidea by affecting membrane permeability

  • Jialiang Pan
  • Xin Hao
  • Hanwen Yao
  • Kangkang Ge
  • Ling MaEmail author
  • Wei MaEmail author
Original Paper
  • 76 Downloads

Abstract

Matrine is a promising botanical antifungal; however, the mechanism underlying the antifungal activity is yet limited. We studied the antifungal activity of matrine and the underlying mechanism in Botryosphaeria dothidea as a model strain. Matrine strongly inhibited mycelial growth of B. dothidea in a dose-dependent manner. Matrine-treated B. dothidea showed morphological and ultrastructural alterations, including shriveled hyphae, plasmolysis, and leakage of cytoplasm related to cell membrane deterioration. In addition, matrine caused significantly high conductivity and absorbance (260 nm) in extracellular matrices and low lipid contents in B. dothidea, indicating increased membrane permeability. Lipid peroxidation showed that matrine resulted in increased malondialdehyde content while enhancing the generation of reactive oxygen species and the activities of superoxide dismutase, catalase, and peroxidase. These results showed that matrine inhibited the mycelial growth of B. dothidea by enhancing cell membrane permeability via membrane lipid peroxidation.

Keywords

Matrine Antifungal activity Botryosphaeria dothidea Membrane permeability Lipid peroxidation 

Notes

Acknowledgements

I am grateful to all my colleagues that assisted in writing this thesis, particularly my supervisor, Dr. Ling Ma, who offered me valuable suggestions in my academic study.

Author’s contribution

Jialiang Pan and Xin Hao are equal Contribution to this paper.

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Copyright information

© Northeast Forestry University 2019

Authors and Affiliations

  1. 1.College of ForestryNortheast Forestry UniversityHarbinPeople’s Republic of China
  2. 2.General Station of Forest and Grassland Pest ManagementNational Forestry and Grassland AdministrationShenyangPeople’s Republic of China
  3. 3.College of PharmacyHeilongjiang University of Chinese MedicineHarbinPeople’s Republic of China

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