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Expression of BpPIN is associated with IAA levels and the formation of lobed leaves in Betula pendula ‘Dalecartica’

  • Chang Qu
  • Xiuyan Bian
  • Rui Han
  • Jing Jiang
  • Qibin Yu
  • Guifeng Liu
Original Paper
  • 13 Downloads

Abstract

Auxin polar transport genes PIN (PIN-FORMED) determine the concentration gradient of auxin in plants. To understand the relationship between the development of different tissues in Betula pendula ‘Dalecartica’, BpPIN gene expression and indole-3-acetic acid (IAA) content were analyzed using qRT-PCR, ELISA, and GUS staining. Gene expression of BpPIN genes and IAA levels in the leaves, buds, stems, xylem, and roots of B. pendula ‘Dalecartica’ and B. pendula as a control were measured. BpPIN1, BpPIN5 and BpPIN6 were upregulated during development in both species, suggesting a dominant role in the development of B. pendula ‘Dalecartica’ leaves. Moreover, BpPIN1 gene expression was positively associated with IAA levels during leaf, vein and petiole development in B. pendula ‘Dalecartica’ only. The correlation coefficient of the first three leaves was 0.69 (P = 0.04), while that of the first three petioles was 0.85 (P = 0.001). In addition, GUS staining of the pro-DR5::GUS transgenic line of cultivar was correlated with the results of BpPIN1 expression. Overall, these findings suggest that BpPIN1 is associated with the formation of lobed leaves in B. pendula ‘Dalecartica’.

Keywords

Betula pendula Betula pendula ‘Dalecartica’ BpPIN genes Gene expression IAA 

References

  1. Adamowski M, Friml J (2015) PIN-dependent auxin transport: action, regulation, and evolution. Plant Cell 27:20–32CrossRefPubMedCentralGoogle Scholar
  2. Ansari SA, Singh S, Rani A (2004) Inorganic salts influence IAA ionization and adventitious rhizogenesis in Pongamia pinnata. J Plant Physiol 161:17–120CrossRefGoogle Scholar
  3. Bilsborough GD, Runions A, Barkoulas M, Jenkins HW, Hasson A, Galinha C, Laufs P, Hay A, Prusinkiewicz P, Tsiantis M (2011) Model for the regulation of Arabidopsis thaliana leaf margin development. Proc Natl Acad Sci USA (PNAS) 108:3424–3429CrossRefGoogle Scholar
  4. Hay A, Barkoulas M, Tsiantis M (2006) ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis. Development 133:3955–3961CrossRefPubMedCentralGoogle Scholar
  5. Hu QY, Zhu ZW, Peng Y, Zhao Y, Huang Y, Liu XZ, Zhang XW (2015) Cloning and characterization of auxin polar transport PIN3 gene cDNA and its promoter in Capsella bursa-pastoris. J China Agric Univ 20(1):29–36Google Scholar
  6. Huang HJ (2014) Function research of AP1 in Betula platyphylla × Betula pendula. Northeast Forestry University, HarbinGoogle Scholar
  7. Kumar P, Jharia SK, Ansari SA (2011) Change in pH regimes and adventitious root induction in semi-hardwood cuttings of Gmelina arborea Roxb. Plant Growth Regul 65:531–536CrossRefGoogle Scholar
  8. Li QJ (2015) Molecular cloning and expression analysis of PIN genes from Adiantum reniforme var.sinense y.x.lin. Huazhong Agricultural University, WuhanGoogle Scholar
  9. Li LC, Zhai LJ (2006) Regulation of leaf development by auxin in Arabidopsi. Chin Bull Bot 23(5):459–465Google Scholar
  10. Liu XZ (2011) The study on the auxin’s distribution during the development of carpel of Capsella bursapastoris. Hunan Agricultural University, ChangshaGoogle Scholar
  11. Liu SP, Wang L, Wang JR, Xue YH, Shou HX (2009) PIN gene family in higher plants. Plant Physiol Commun 45(8):833–841Google Scholar
  12. Liu FL, Kou YP, Chen XL, Gao B, Wang L, Zhao LJ (2014) Cloning and expression analysis of PIN1 and PIN2 encoding auxin efflux carriers in Rosa canina. Acta Hortic Sin 41(05):925–934Google Scholar
  13. Mu HZ, Lin L, Liu GF, Jiang J (2013) Transcriptomic analysis of incised leaf-shape determination in birch. Gene 531:263–269CrossRefPubMedCentralGoogle Scholar
  14. Okada K, Ueda J, Komaki MK, Bell CJ, Shimura Y (1991) Requirement of the auxin polar transport system in early stages of Arabidopsis floral bud formation. Plant Cell 3:677–684CrossRefPubMedCentralGoogle Scholar
  15. Peer WA, Bandyopadhyay A, Blakeslee JJ, Makam SN, Chen RJ, Masson PH, Murphy AS (2004) Variation in expression and protein localization of the PIN family of auxin efflux facilitator proteins in flavonoid mutants with altered auxin transport in Arabidopsis thaliana. Plant Cell 16:1898–1911CrossRefPubMedCentralGoogle Scholar
  16. Qu C, Bian XY, Jiang J, Chen S, Liu GF (2017) Leaf morphological characteristics and related gene expression characteristics analysis in Betula pendula ‘Dalecarlica’ and Betula pendula. J Beijing For Univ 39(8):9–16Google Scholar
  17. Rousi M, Pusenius J (2005) Variations in phenology and growth of European white birch (Betula pendula) clones. Tree Physiol 25:201–210CrossRefPubMedCentralGoogle Scholar
  18. Valobra CP, James DJ (1990) In vitro shoot regeneration from leaf discs of Betula pendula ‘Dalecarlica’ EM 85. Plant Cell Tissue Organ Cult 21:51–54CrossRefGoogle Scholar
  19. Wang W, Li DQ, Yang XH, Zou Q, Zhou X, Yang J (2000) Effects of water stress on level changes of IAA and ABA in rootand shoot of different drought resistance wheat. Acta Agron Sin 26(6):737–742Google Scholar
  20. Wang ZJ, Yang LW, Xu ZD, Ou ZL, Yuan HL, Ren YF, Qi LL, He ZA, Chen YS (2015) Identification and bioinformatics analysis of the PIN gene family in Jatropha curcas. Mol Plant Breed 13(05):1111–1122Google Scholar
  21. Wang B, Cao HL, Huang YT, Hu YR, Qian WJ, Hao XY, Wang L, Yang YJ, Wang XC (2016) Cloning and expression analysis of auxin efflux carrier gene CsPIN3 in tea plant (Camellia sinensis). Acta Agron Sin 42(1):58–69CrossRefGoogle Scholar
  22. Yang G (2015) Function of BpGH3.5 gene in Betula platyphylla × Betula pendula. Northeast Forestry University, HarbinGoogle Scholar
  23. Yang G, Chen S, Wang S, Liu GF, Li HY, Huang HJ, Jiang J (2015a) BpGH3.5, an early auxin-response gene, regulates root elongation in Betula platyphylla × Betula pendula. Plant Cell Tissue and Organ Cult 120(1):239–250CrossRefGoogle Scholar
  24. Yang GY, Zhao Z, Zhao YL, Zhang FJ, Gao CQ (2015b) Upstreamregulators of ThVHAc1. J Beijing For Univ 37(12):1–6Google Scholar
  25. Zhang RP (2009) Study on expression pattern of ThDHN responding to different abiotic stress and establishment of genetic transformation of Betula platyphylla. Northeast Forestry University, HarbinGoogle Scholar
  26. Zhao YC (2009) Research on function of miR164 in Thellungiella salsuginea. Master’s Thesis of Shandong Normal UniversityGoogle Scholar
  27. Zhou CN, Han L, Hou CY, Metelli A, Qi LY, Tadege M, Mysore KS, Wang ZY (2011) Developmental analysis of a Medicago truncatula smooth leaf margin1 mutant reveals context-dependent effects on compound leaf development. Plant Cell 23:2106–2124CrossRefPubMedCentralGoogle Scholar
  28. Zhu ZW, Peng Y, Zhao Y, Hu QY, Zhang XW (2013) Quantitative analysis of expression of auxin polar transport PIN genes in different tissues of Arabidopsis thaliana and Capsella bursa-pastoris. J Hunan Agric Univ (Nat Sci) 39(05):495–499Google Scholar

Copyright information

© Northeast Forestry University 2018

Authors and Affiliations

  • Chang Qu
    • 1
  • Xiuyan Bian
    • 1
  • Rui Han
    • 1
  • Jing Jiang
    • 1
  • Qibin Yu
    • 2
  • Guifeng Liu
    • 1
  1. 1.State Key Laboratory of Tree Genetics and BreedingNortheast Forestry UniversityHarbinPeople’s Republic of China
  2. 2.University of FloridaLake AlfredUSA

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