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Biochemical composition and photosynthetic activity of Pongamia pinnata (L.) Pierre in response to acute 60Co γ-irradiation

  • Mohd Rafi Wani
  • S. S. Singh
  • Vandana Sharma
Original Paper
  • 11 Downloads

Abstract

To elucidate the potential and sensitivity of γ-irradiation in Pongamia pinnata, the present study has been done by irradiating the air-dried seeds to different γ-irradiation doses (100 Gy, 200 Gy, 400 Gy and 600 Gy), using 60Co source. Significant increase (p ≤ 0.05) in the germination, growth, and vigor was recorded under the 100 Gy treatment than the control set. The chlorophylla and total chlorophyll content (mg g−1 FW) in the leaves of P. pinnata showed a significant decrease under the higher irradiation treatments (200 Gy, 400 Gy and 600 Gy). In contrast, chlorophyllb showed a radio-resistance up to 200 Gy dose, and above which its concentration declined significantly (p ≤ 0.05). Photosynthetic rate, stomatal conductance, intercellular CO2 concentration, and transpiration rate were stimulated by 100 Gy irradiation treatment and the higher doses inhibited these parameters. Antioxidant activity in the leaves of P. pinnata tended to increase after irradiation in a dose-dependent manner. All the plants under different treatments of γ-irradiation showed stimulation in production of proline, flavonoid, and phenolic content in comparision to the control. The findings of the present study showed that γ-irradiation treatment stimulates the secondary metabolite production (proline, flavonoid and phenolic) and favours faster growth of P. pinnata.

Keywords

Biochemical composition γ-irradiation Photosynthetic rate Pongamia pinnata Principal component analysis 

Abbreviations

A

Photosynthetic rate

Ci

Inter cellular CO2 concentration

60Co

Cobalt-60 (A synthetic radioactive isotope of cobalt)

E

Transpiration rate

Gy

Gray

GEI

Germination energy index

GP

Germination percentage

GS

Stomatal conductance

GV

Germination value

PCA

Principal component analysis

ROS

Reaction oxygen species

Notes

Acknowledgements

Authors are thankful to RTM Nagpur University (Department of Chemistry) for providing irradiation facility.

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Copyright information

© Northeast Forestry University and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Forestry, Wildlife and Environmental SciencesGuru Ghasidas VishwavidyalayaBilaspurIndia
  2. 2.HNB Garhwal University CampusTehri, GarhwalIndia

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