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Journal of Forestry Research

, Volume 30, Issue 6, pp 1997–2005 | Cite as

Shoot proliferation and callus regeneration from nodular buds of Drepanostachyum luodianense

  • Shuyan Lin
  • Guohua Liu
  • Tingting Guo
  • Li Zhang
  • Shuguang WangEmail author
  • Yulong DingEmail author
Original Paper
  • 81 Downloads

Abstract

This research reports on an efficient shoot proliferation and callus regeneration system for bamboo. Young, semi-lignified branches with one lateral bud from Drepanostachyum luodianense (Yi et R. S. Wang) Keng f. were used as explants. Disinfection with 0.1% HgCl2 for 8 min was the optimum treatment and the best medium for bud initiation was Murashige and Skoog (MS) medium containing 3.0 mg L−1 6-benzyladenine (BA). Multiple shoots were induced from nodal shoot segments on MS medium containing 5.0 mg L−1 BA, 0.5 mg L−1 kinetin (Kin), and 1.0 mg L−1 naphthaleneacetic acid (NAA). The highest frequency of callus formation (65.6%) was on MS medium containing 4.0 mg L−1 2,4-dichlorophenoxyacetic acid (2, 4-D), 0.5 mg L−1 NAA, and 0.1 mg L−1 thidiazuron (TDZ). The optimum medium for callus proliferation was MS medium with 4 mg L−1 2,4-D, 0.5 mg L−1 TDZ and 0.5 mg L−1 NAA, and the optimum hormone combination was 4 mg L−1 BA + 0.5 mg L−1 NAA for callus redifferentiation (up to 85.6%). A 100% rooting was achieved on MS medium supplemented with 2.0 mg L−1 NAA and 0.5 mg L−1 3-indole butyric acid (IBA). Rooted plantlets were acclimatized in a greenhouse in humus soil + perlite (1:1) substrate. These micropropagated callus induction and regeneration systems for bamboo will be useful for genetic engineering and multiplication.

Keywords

Micropropagation Bamboo Shoot proliferation Callus regeneration Transplantation 

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Copyright information

© Northeast Forestry University and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Co-Innovation Center for Sustainable Forestry in Southern China, Bamboo Research Institute, College of Biology and the EnvironmentNanjing Forestry UniversityNanjingPeople’s Republic of China
  2. 2.College of Life ScienceSouthwest Forestry UniversityKunmingPeople’s Republic of China

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