Drought stress tolerance analysis of Populus ussuriensis clones with different ploidies
- 56 Downloads
The selection of drought-tolerant plants is an important aspect of plant breeding. We studied physiological and biochemical mechanisms of different ploidies of Populus ussuriensis Kom. that relate to drought stress tolerance. We used a 5% (v/v) polyethylene glycol (PEG-6000) solution to simulate drought stress. We recorded leaf phenotypes including color, dry area and curl degree. We evaluated sequential variations in some drought stress tolerance-related physiological and biochemical indices and compared these among diploid clones (CK), triploid clones (T12) and tetraploid clones (F20). T12 leaves exhibited slightly more drought stress damage than CK and F20 leaves. CK leaves suffered the most severe drought stress damage. The physiological and biochemical indices of the different ploidies differed significantly 12 days after drought stress treatment. The activities of superoxide dismutase, peroxidase, catalase and proline in the triploid (T12) leaves were the highest. The relative electric conductivity and malondialdehyde content of T12 leaves were the lowest. The index values of F20 were between those of the diploid and triploid. In consideration of these results, the drought resistance of the three different ploidies of P. ussuriensis can be ranked as T12 > F20 > CK. We speculate that the gene expression patterns of polyploid clones of poplar will change after genome doubling and that some of the drought stress tolerance-related physiological and biochemical indices will be improved, resulting in greater drought tolerance of polyploid clones.
KeywordsPopulus ussuriensis Polyploidy Drought stress Tolerance
This work was supported by the National Key R&D Program of China (Grant No. 2016YFD0600404).
JX and KL: conceived and designed the experiments; JX, JJ and HZ: performed the experiments; JX and HZ: analyzed the data; KL: contributed reagents/materials/analysis tools; JX: wrote the paper; HZ: critically read the manuscript.
Compliance with ethical standards
Conflict of interest
There is no interest conflict.
- Abuduwaili J, Zhang ZY, Jiang FQ, Liu DW (2015) The disastrous effects of salt dust deposition on cotton leaf photosynthesis and the cell physiological properties in the Ebinur Basin in Northwest China. PloS one 10:e0124546. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4430278/
- Anjum SA, Xie XY, Wang LC, Saleem MF, Man C, Lei W (2011) Morphological, physiological and biochemical responses of plants to drought stress. Afr J Agric Res 6:2026–2032Google Scholar
- Eifler I (1960) The individual results of crosses between B. verrucosa and B. pubescens. Silvae Genetica 9:159–165Google Scholar
- Fang GG, Deng YJ, Li P (2001) Evaluation of pulping performance of triploid Populus tomentosa. For Sci Technol Manag (Suppl.):87–90Google Scholar
- Gupta B, Huang B (2014) Mechanism of salinity tolerance in plants: physiological, biochemical, and molecular characterization. Int J Genomics. Article ID 701596. https://www.researchgate.net/publication/262114029_Mechanism_of_Salinity_Tolerance_in_Plants_Physiological_Biochemical_and_Molecular_Characterization. Accessed 3 Apr 2014
- Hasheminasab H, Assad MT, Aliakbari A, Sahhafi SR (2012) Influence of drought stress on oxidative damage and antioxidant defense systems in tolerant and susceptible wheat genotypes. J Agric Sci 4:20Google Scholar
- Johnsson H (1944) Triplody in Betula alba L. Bot Notiser Lund 1:85–96Google Scholar
- Lisar SYS, Motafakkerazad R, Hossain MM, Rahman IMM (2012) Water stress in plants: causes, effects and responses. In: Rahman IMM (ed) Water stress, vol 175–178(117). InTech, London, pp 635–642Google Scholar
- Liu W, Yu K, He T, Li F, Zhang D, Liu J (2013) The low temperature induced physiological responses of Avena nuda L., a cold-tolerant plant species. Sci World J. Article ID 658793. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3693167/. Accessed 11 June 2013
- Michalak A (2006) Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish J Environ Stud 15:523–530Google Scholar
- Osmond CB, Bjorkman O, Anderson DJ (1980) Physiological processes in plant ecology. Toward a synthesis with atriplex. Agro-Ecosystems 8(3):268–271Google Scholar
- Pembrey RS, Marshall KC, Schneider RP (1999) Cell surface analysis techniques: What do cell preparation protocols do to cell surface properties? Appl Environ Microbiol 65:2877–2894Google Scholar
- Sutka J, Farshadfar E, Kőszegi B, Friebe B, Gill BS (1995) Drought tolerance of disomic chromosome additions of Agropyron elongatum to Triticum aestivum. Cereal Res Commun 23:351–357Google Scholar
- Zhu ZT, Kang XY (1998) Studies on selection of natural triploids of Populus tomentosa. Scientia Silvae Sinicae 34:22–31Google Scholar