Gene cloning and expression analysis of limonene synthase in Syringa oblata and S. oblata var. alba
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Syringa species are important ornamentals with strong floral scent, of which monoterpenes are the main component. In this study, a new monoterpene synthase gene, named SoLIM, was collected from the flowers of Syringa oblata and S. oblata var. alba using a homologous cloning method. The full-length cDNA of SoLIM was 1746 bp and encoded 581 amino acids. Sequence analysis showed that SoLIM contained the DDxxD and RRx8W motifs, which are two typical conserved monoterpene synthase motifs, and was thus classified as belonging to the Tpsb subfamily. Using quantitative reverse-transcription PCR, SoLIM was significantly expressed in the petals and pistils of S. oblata and S. oblata var. alba, respectively. SoLIM expression peaked earlier than the D-limonene emissions in the diurnal experiments, but occurred later when D-limonene had peaked during the flowering phase, indicating that differences in SoLIM gene expression and D-limonene emissions existed. The synthesis of floral scent is thus associated with diverse regulatory mechanisms that require further investigation.
KeywordsFloral scent Syringa oblata Syringa oblata var. alba Limonene synthase gene
We would like to thank LetPub (www.letpub.com) for providing linguistic assistance during the preparation of this manuscript.
- Alonso WR, Rajaonarivony JI, Gershenzon J, Croteau R (1992) Purification of 4S-limonene synthase, a monoterpene cyclase from the glandular trichomes of peppermint (Mentha × piperita) and Spearmint (Mentha spicata). Biol Chem 267:7582–7587Google Scholar
- Byun-McKay A, Godard KA, Toudefallah M, Martin DM, Alfaro R, King J, Bohlmann J, Plant AL (2006) Wound-induced terpene synthase gene expression in Sitka Spruce that exhibit resistance or susceptibility to attack by the white Pine weevil. Plant Physiol 140:1009–1021CrossRefPubMedPubMedCentralGoogle Scholar
- Dudareva N, Martin D, Kish CM, Kolosova N, Gorenstein N, Faldt J, Miller B, Bohlmann J (2003) (E)-β-Ocimene and myrcene synthase genes of floral scent biosynthesis in Snapdragon: function and expression of three terpene synthase genes of a new terpene synthase subfamily. Plant Cell 15:1227–1241CrossRefPubMedPubMedCentralGoogle Scholar
- Hu ZH, Li TJ, Zheng J, Leng PS, Yang K, Zhang KZ (2016) A new monoterpene synthase gene involved in the monoterpene production from Lilium ‘siberia’. J Anim Plant Sci 26:1389–1398Google Scholar
- Iijima Y, Davidovich-Rikanati D, Fridman E, Gang DR, Bar E, Lewinsohn E, Pichersky E (2004) The biochemical and molecular basis for the divergent patterns in the biosynthesis of terpenes and phenylpropenes in the peltate glands of three cultivars of Basil. Plant Physiol 136:3724–3736CrossRefPubMedPubMedCentralGoogle Scholar
- Li TJ, Hu ZH, Zheng J, Leng PS, Yang K (2016) Diurnal changes of monoterpenes emission and Li-mTPS expression in Lilium ‘siberia’. Acta Agric Boreali Occident Sin 25:763–769Google Scholar
- Qin Y, Yang XX, Leng PS, Hu ZH (2015) Analysis of floral volatile components from 6 Syringa plants collected by dynamic headspace sampling by ATD-GC/MS. Acta Bot Boreal Occident Sin 35:2078–2088Google Scholar
- Wang JH, Dudareva N, Bhakta S, Raguso RA, Pichersky E (1997) Floral scent production in Clarkia breweri (Onagraceae): II. Localization and developmental modulation of the enzyme s-adenosyl-l-methionine: (iso) eugenol O-methyltransferase and phenylpropanoid emission. Plant Physiol 114:213–221CrossRefPubMedPubMedCentralGoogle Scholar
- Zhao J, Hu ZH, Leng PS, Zhang HX, Cheng FY (2012) Fragrance composition in six tree peony cultivars. Kor J Hort Sci Technol 30:617–625Google Scholar