Gene cloning and expression analysis of limonene synthase in Syringa oblata and S. oblata var. alba

  • Zifei Yan
  • Ying Qin
  • Jian Zheng
  • Pingsheng Leng
  • Zenghui HuEmail author
Original Paper


Syringa species are important ornamentals with strong floral scent, of which monoterpenes are the main component. In this study, a new monoterpene synthase gene, named SoLIM, was collected from the flowers of Syringa oblata and S. oblata var. alba using a homologous cloning method. The full-length cDNA of SoLIM was 1746 bp and encoded 581 amino acids. Sequence analysis showed that SoLIM contained the DDxxD and RRx8W motifs, which are two typical conserved monoterpene synthase motifs, and was thus classified as belonging to the Tpsb subfamily. Using quantitative reverse-transcription PCR, SoLIM was significantly expressed in the petals and pistils of S. oblata and S. oblata var. alba, respectively. SoLIM expression peaked earlier than the D-limonene emissions in the diurnal experiments, but occurred later when D-limonene had peaked during the flowering phase, indicating that differences in SoLIM gene expression and D-limonene emissions existed. The synthesis of floral scent is thus associated with diverse regulatory mechanisms that require further investigation.


Floral scent Syringa oblata Syringa oblata var. alba Limonene synthase gene 



We would like to thank LetPub ( for providing linguistic assistance during the preparation of this manuscript.


  1. Alonso WR, Rajaonarivony JI, Gershenzon J, Croteau R (1992) Purification of 4S-limonene synthase, a monoterpene cyclase from the glandular trichomes of peppermint (Mentha × piperita) and Spearmint (Mentha spicata). Biol Chem 267:7582–7587Google Scholar
  2. Byun-McKay A, Godard KA, Toudefallah M, Martin DM, Alfaro R, King J, Bohlmann J, Plant AL (2006) Wound-induced terpene synthase gene expression in Sitka Spruce that exhibit resistance or susceptibility to attack by the white Pine weevil. Plant Physiol 140:1009–1021CrossRefPubMedPubMedCentralGoogle Scholar
  3. Chappell J (1995) The biochemistry and molecular biology of isoprenoid metabolism. Plant Physiol 107:1–6CrossRefPubMedPubMedCentralGoogle Scholar
  4. Dudareva N, Cseke L, Blanc VM, Pichersky E (1996) Evolution of floral scent in Clarkia: novel patterns of S-linalool synthase gene expression in the C. breweri flower. Plant Cell 8:1137–1148CrossRefPubMedPubMedCentralGoogle Scholar
  5. Dudareva N, Murfitt LM, Mann CJ, Gorenstein N, Kolosova N, Kish CM, Bonham C, Wood K (2000) Developmental regulation of methyl benzoate biosynthesis and emission in Snapdragon flowers. Plant Cell 12:949–961CrossRefPubMedPubMedCentralGoogle Scholar
  6. Dudareva N, Martin D, Kish CM, Kolosova N, Gorenstein N, Faldt J, Miller B, Bohlmann J (2003) (E)-β-Ocimene and myrcene synthase genes of floral scent biosynthesis in Snapdragon: function and expression of three terpene synthase genes of a new terpene synthase subfamily. Plant Cell 15:1227–1241CrossRefPubMedPubMedCentralGoogle Scholar
  7. Fu JX, Hou D, Zhang C, Bao ZY, Zhao HB, Hu SQ (2017) The emission of the floral scent of four Osmanthus fragrans cultivars in response to different temperatures. Molecules 22:430CrossRefGoogle Scholar
  8. Hu ZH, Zhang HX, Leng PS, Zhao J, Wang WH, Wang SD (2013) The emission of floral scent from Lilium ‘siberia’ in response to light intensity and temperature. Acta Physiol Plant 35:1691–1700CrossRefGoogle Scholar
  9. Hu ZH, Li TJ, Zheng J, Leng PS, Yang K, Zhang KZ (2016) A new monoterpene synthase gene involved in the monoterpene production from Lilium ‘siberia’. J Anim Plant Sci 26:1389–1398Google Scholar
  10. Huang KF, Lee YR, Tseng YH, Wang SY, Chu FH (2015) Cloning and functional characterization of a monoterpene synthase gene from Eleutherococcus trifoliatus. Holzforschung 69:163–171CrossRefGoogle Scholar
  11. Iijima Y, Davidovich-Rikanati D, Fridman E, Gang DR, Bar E, Lewinsohn E, Pichersky E (2004) The biochemical and molecular basis for the divergent patterns in the biosynthesis of terpenes and phenylpropenes in the peltate glands of three cultivars of Basil. Plant Physiol 136:3724–3736CrossRefPubMedPubMedCentralGoogle Scholar
  12. Kim SY, An HR, Park PM, Baek YS, Kwon OK, Park SY, Park PH (2016) Analysis of floral scent patterns in flowering stages and floral organs of Maxillaria using an electronic nose. Flower Res J 24:171–180CrossRefGoogle Scholar
  13. Knudsen JT, Tollsten L, Bergstrom LG (1993) Floral scents—a checklist of volatile compounds isolated by head-space techniques. Phytochemistry 33:253–280CrossRefGoogle Scholar
  14. Knudsen JT, Eriksson R, Gershenzon J, Stahl B (2006) Diversity and distribution of floral scent. Bot Rev 72:1–120CrossRefGoogle Scholar
  15. Kolosova N, Gorenstein N, Kish CM, Dudareva N (2001) Regulation of circadian methyl benzoate emission in diurnally and nocturnally emitting plants. Plant Cell 13:2333–2347CrossRefPubMedPubMedCentralGoogle Scholar
  16. Li TJ, Hu ZH, Zheng J, Leng PS, Yang K (2016) Diurnal changes of monoterpenes emission and Li-mTPS expression in Lilium ‘siberia’. Acta Agric Boreali Occident Sin 25:763–769Google Scholar
  17. McGarvey DJ, Croteau R (1995) Terpenoid metabolism. Plant Cell 7:1015–1026CrossRefPubMedPubMedCentralGoogle Scholar
  18. Mendoza-Poudereux I, Munoz-Bertomeu J, Navarro A, Arrillaga I, Segura J (2014) Enhanced levels of S-linalool by metabolic engineering of the terpenoid pathway in spike lavender leaves. Metab Eng 23:136–144CrossRefPubMedGoogle Scholar
  19. Paré PW, Tumlinson JH (1997) De novo biosynthesis of volatiles induced by insect herbivory in cotton plants. Plant Physiol 114:1161–1167CrossRefPubMedPubMedCentralGoogle Scholar
  20. Qin Y, Yang XX, Leng PS, Hu ZH (2015) Analysis of floral volatile components from 6 Syringa plants collected by dynamic headspace sampling by ATD-GC/MS. Acta Bot Boreal Occident Sin 35:2078–2088Google Scholar
  21. Shimada T, Endo T, Fujii H, Omura M (2005) Isolation and characterization of a new D-limonene synthase gene with a different expression pattern in Citrus unshiu Marc. Sci Hortic 105:507–512CrossRefGoogle Scholar
  22. Su GZ, Cao Y, Li C, Yu XL, Gao XL, Tu PF, Chai XY (2015) Phytochemical and pharmacological progress on the genus Syringa. Chem Cent J 9:2CrossRefPubMedPubMedCentralGoogle Scholar
  23. Sun HN, Zhang T, Fan QQ, Qi XY, Zhang F, Fang WM, Jiang JF, Chen FD, Chen SM (2015) Identification of floral scent in Chrysanthemum cultivars and wild relatives by gas chromatography-mass spectrometry. Molecules 20:5346–5359CrossRefPubMedGoogle Scholar
  24. Wang JH, Dudareva N, Bhakta S, Raguso RA, Pichersky E (1997) Floral scent production in Clarkia breweri (Onagraceae): II. Localization and developmental modulation of the enzyme s-adenosyl-l-methionine: (iso) eugenol O-methyltransferase and phenylpropanoid emission. Plant Physiol 114:213–221CrossRefPubMedPubMedCentralGoogle Scholar
  25. Wang HT, Yu X, Liu Y, Liang CY, Li WL (2013) Analysis of genetic variability and relationships among Mentha L. using the limonene synthase gene, LS. Gene 524:246–252CrossRefPubMedGoogle Scholar
  26. Yang XX, Zhao J, Zheng J, Leng PS, Li XL, Hu ZH, Liu JB, Meng X (2016) Analysis of floral scent emitted from Syringa plants. J For Res 27:273–281CrossRefGoogle Scholar
  27. Yue YC, Yu RC, Fan YP (2014) Characterization of two monoterpene synthases involved in floral scent formation in Hedychium coronarium. Planta 240:745–762CrossRefPubMedGoogle Scholar
  28. Zhang JH, Sun HL, Chen SY, Zeng L, Wang TT (2017) Anti-fungal activity, mechanism studies on α-phellandrene and nonanal against Penicillium cyclopium. Bot Stud 58:13CrossRefPubMedPubMedCentralGoogle Scholar
  29. Zhao J, Hu ZH, Leng PS, Zhang HX, Cheng FY (2012) Fragrance composition in six tree peony cultivars. Kor J Hort Sci Technol 30:617–625Google Scholar

Copyright information

© Northeast Forestry University and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Zifei Yan
    • 1
  • Ying Qin
    • 1
  • Jian Zheng
    • 1
    • 2
  • Pingsheng Leng
    • 1
    • 2
  • Zenghui Hu
    • 1
    • 2
    Email author
  1. 1.College of Landscape ArchitectureBeijing University of AgricultureBeijingPeople’s Republic of China
  2. 2.Beijing Collaborative Innovation Center for Eco-environmental Improvement with Forestry and Fruit TreesBeijingPeople’s Republic of China

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