Effect of the thin cell layer technique in the induction of somatic embryos in Pinus patula Schl. et Cham
Pinus patula is a species commonly used for reforestation in Mexico. However, efficient methods for the mass production seedlings are required. Micropropagation particularly by somatic embryogenesis provides an option for the rapid multiplication of high-quality, genetically improved material. This study induces somatic embryogenesis in this species using the thin cell layer (TCL) technique. Two sources of explants (complete immature embryos; lTCL segments from immature embryos) were evaluated. The efficiency of TCL from longitudinal sections [lTCL] and transverse [tTCL] was evaluated. The results show using thin cell layers from immature embryos cultivated in 16 light/8 dark hours achieves induction of somatic embryos. A higher percentage of embryogenic callus was obtained when tTCL segments were used as an explant source. These results produced somatic embryos from tTCL segments of an immature embryo without germinating the seed, making the process more time efficient. In addition, this technique can be used to generate somatic embryogenesis in forest species that have low germination rates.
KeywordsPinus patula Somatic embryos Micropropagation Thin cell layer Maturation
LGIA, MARM and JFTS conceived and designed research. MARM, EJCG and AAAS conducted experiments. MARM and AAAS analyzed and reviewed the statistical analysis. MARM, ORLO and CMGP performed the histological analyzes. MARM and LGIA wrote the manuscript. LGIA, MARM, AAAS, EJCG, and JFTS read and approved the manuscript.
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