Journal of Forestry Research

, Volume 30, Issue 3, pp 807–815 | Cite as

An efficient in vitro shoot regeneration through direct organogenesis from seedling-derived petiole and leaf segments and acclimatization of Ficus religiosa

  • Mohsen HesamiEmail author
  • Mohammad Hosein Daneshvar
  • Mohsen Yoosefzadeh-Najafabadi
Original Paper


Prolific and rapid in vitro plant organogenesis via direct regeneration has been obtained from axenic seedling-derived petiole and leaf explants of Ficus religiosa in Murashige and Skoog (MS) medium containing different concentrations of cytokinins in combination with indole-3-butyric acid (IBA). MS medium with 1.5 mg/l 6-benzylaminopurine plus 0.15 mg/l IBA produced the highest shoot induction frequency with an average of 6.26 and 10.13 shoots per leaf and petiole explants, respectively. After 4 weeks, the highest root formation frequency (96.7%), root number (5.73), and root length (4.76 cm) were with MS medium containing 2.0 mg/l IBA plus 0.1 mg/l α-naphthalene acetic acid. In addition, the effect of four sodium nitroprusside (SNP) treatments on acclimatization was also studied. Highest morphological traits such as survival rates, fresh and dry root weights as well as antioxidant enzymatic activities such as superoxide dismutase, peroxidase, and catalase was achieved with 125 ppm SNP. The α-amino acid, proline, content was highest with this treatment while the highest H2O2 (hydrogen peroxide) was in the controls. This study introduces a cost-effective, prolific, and efficient in vitro multiplication system to supply pharmaceutical and ornamental needs. It is the first report of an in vitro organogenesis protocol for F. religiosa by direct regeneration through axenic seedling-derived petiole and leaf explants, which can be efficiently employed for the utilization of active biomolecules.


In vitro culture Ficus religiosa Sodium nitroprusside Growth regulators Antioxidant enzymes Proline 


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Copyright information

© Northeast Forestry University and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Mohsen Hesami
    • 1
    Email author
  • Mohammad Hosein Daneshvar
    • 2
  • Mohsen Yoosefzadeh-Najafabadi
    • 1
  1. 1.Department of Horticulture ScienceUniversity of TehranKarajIran
  2. 2.Department of Horticulture ScienceRamin University of Agriculture and Natural ResourcesAhwazIran

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