Journal of Forestry Research

, Volume 30, Issue 1, pp 87–93 | Cite as

Micropropagation protocol for Salvadora oleoides

  • Ravindra KumarEmail author
  • Raoof Ahmad Najar
  • Kunj Bihari Gupta
  • Ram Gopal Saini
Original Paper


Salvadora oleoides Decne. is a pharmaceutically important plant. Owing to poor seed formation, viability and, germination, and to anthropogenic disturbances, this species is on the verge of extinction. A reproducible micropropagation protocol to increase the population through tissue culture has been standardized and the results are reported here. Callus tissues were initiated from young leaves and stem explants. Leaf calluses proliferated with 1.5 mg/L BAP and 0.9 mg/L 2, 4-D with additives and continuous slow proliferation up to 15 weeks on 0.5 mg/L BAP and additives with 200 mg/L activated charcoal. Direct shoot initiation took place from stem node explants after 12 days; 4–5 shoots per node were produced in 30 days. Shoot clumps elongated and grew further on MS media supplemented with 2 mg/L BAP, 0.2 mg/L NAA and additives, which generated 20–23 shoots. The elongated shoots induced tap roots with 4 mg/L NAA and 200 mg/L activated charcoal in 12 days. In vitro raised plants produced secondary roots when transferred to pots containing vermiculite maintained at 28–35 °C. The plantlets successfully acclimatised in pots containing soil in natural conditions.


Salvadora oleoides Endangered species Micropropagation Explants Calluses Acclimatize 



The first author acknowledges the financial support from the Central University of Punjab, Bathinda during the study.


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Copyright information

© Northeast Forestry University and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Ravindra Kumar
    • 1
    Email author
  • Raoof Ahmad Najar
    • 2
  • Kunj Bihari Gupta
    • 1
  • Ram Gopal Saini
    • 3
  1. 1.Department of Biochemistry and Microbial Sciences, School of Basic and Applied SciencesCentral University of PunjabBathindaIndia
  2. 2.Department of Plant Sciences, School of Basic and Applied SciencesCentral University of PunjabBathindaIndia
  3. 3.Centre for Interdisciplinary Biomedical ResearchAdesh UniversityBathindaIndia

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