Journal of Forestry Research

, Volume 29, Issue 6, pp 1769–1774 | Cite as

First report of leaf-spot disease caused by Sphaeropsis visci on Asian mistletoe [Viscum coloratum (Kom.) Nakai] in China

  • Jie Chen
  • Xuefeng LiuEmail author
  • Hanqi Jia
  • Wenbo Zhu
Original Paper


In January 2017, symptoms of a leaf-spot disease were observed on Viscum coloratum plants in Yichun, China. The infected leaves were chlorotic, while sunken lesions formed on diseased branches, which were initially light brown and later turned dark brown. Moreover, diseased branches and leaves formed semi-buried, small, and spherical pycnidia. In total, 20 leaves and 19 branches diseased samples from Yichun, China were collected and examined. The fungus was isolated and identified using Koch’s postulates, morphological characteristics and DNA sequence data. The data presented herein confirmed that the pathogen responsible for the disease was Sphaeropsis visci. To the best of our knowledge, this is the first report of it in China.


Botryosphaeriales Viscum coloratum Morphology Phylogeny Sphaeropsis visci New pathogen 



We thank all my colleagues who helped in writing this thesis.


  1. Ahvenniemi P, Wolf M, Lehtonen MJ, Wilson P, German-Kinnari M, Valkonen JPT (2009) Evolutionary diversification indicated by compensatory base changes in ITS2 secondary structures in a complex fungal species, Rhizoctonia solani. J Mol Evol 69(2):150–163CrossRefGoogle Scholar
  2. Calder M, Bernhardt P (1983) The biology of mistletoes. Academic Press, Sydney, p 348Google Scholar
  3. Chai RY, Jin MZ (1991) Describe a method for direct selection of monospora to obtain monospora strains. Plant Prot 3:53–54Google Scholar
  4. Grover DW, Nicole JM (1940) The vapour pressure of glycerin solutions at twenty degrees. J Soc Chem Ind 59:175–177Google Scholar
  5. McNeill J (2012) International code of nomenclature for algae, fungi and plants (Melbourne code): adopted by the Eighteenth International Botanical Congress Melbourne, Australia, July 2011, vol 154. Koeltz Scientific Books, Königstein, pp 1–240Google Scholar
  6. Phillips AJL, Alves A, Pennycook SR, Johnston PR, Ramaley A, Akulov A, Crous PW (2008) Resolving the phylogenetic and taxonomic status of dark-spoted teleomorph genera in the Botryosphaeriaceae. Persoonia 21:29–55CrossRefGoogle Scholar
  7. Phillips AJL, Alves A, Abdollahzadeh J, Slippers B, Wingfield MJ, Groenewald JZ, Crous PW (2013) The Botryosphaeriaceae: genera and species known from culture. Stud Mycol 76:51–167CrossRefGoogle Scholar
  8. Poczai P, Varga I, Hyvonen J (2015) Internal transcribed spacer (ITS) evolution in populations of the hyperparasitic European mistletoe pathogen fungus, Sphaeropsis visci (Botryosphaeriaceae): the utility of ITS2 secondary structures. Gene 558:54–64CrossRefGoogle Scholar
  9. Qiu HX (1988) Flora of China: volume 24: Mistletoe. Science Press, Beijing, pp 148–149Google Scholar
  10. Reid N, Yan ZG, Fittler J (1994) Impact of mistletoes (Amyema miquelii) on host (Eucalyptus blakelyi and Eucalyptus melliodora) survival and growth in temperate Australia. For Ecol Manag 70:55–65CrossRefGoogle Scholar
  11. Stojanović S (1989) The investigation of Shaeropsis visci (Salm.) Sacc Colletotrichum gloeosporoides (Sacc.) Penz, parasite on European (Viscum album ssp. typicum Beck). Zaštita Bilja 40:493–503Google Scholar
  12. Sutton BC (1980) The Coelomycetes: fungi imperfecti with pycnidia, acervuli, and stromata. Kew, Surrey, Commonwealth Mycological Institute, p 696Google Scholar
  13. Varga I, Taller J, Baltazar T, Hyvonen J, Poczai P (2012) Leaf-spot disease on European mistletoe (Viscum album) caused by Phaeobotryosphaeria visci: a potential candidate for biological control. Biotechnol Lett 34(6):1059–1065CrossRefGoogle Scholar
  14. Varga I, Poczai P, Tiborcz V, Aranyi NR, Baltazár T, Bartha D, Pejchal M, Hyvönen J (2014) Changes in the distribution of the European mistletoe (Viscum album L.) in Hungary during the last hundred years. Folia Geobot 49(4):559–577CrossRefGoogle Scholar
  15. White TJ, Bruns T, Lee S (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA (ed) PCR protocol: a guide to methods and applications. Academic Press, New York, pp 315–322Google Scholar
  16. Zlatkovic M, Keca N, Wingfield MJ, Jami F, Slippers B (2016) Botryosphaeriaceae associated with the die-back of ornamental trees in the Western Balkans. Antonie Van Leeuwenhoek Int General Mol Microbiol 109:543–564CrossRefGoogle Scholar
  17. Zuber D (2004) Biological flora of central Europe: Viscam album L. FLORA 199(3):181–203CrossRefGoogle Scholar

Copyright information

© Northeast Forestry University and Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  1. 1.The Laboratory of Prevention and ControlNortheast Forestry UniversityHarbinPeople’s Republic of China

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