First report of leaf-spot disease caused by Sphaeropsis visci on Asian mistletoe [Viscum coloratum (Kom.) Nakai] in China
In January 2017, symptoms of a leaf-spot disease were observed on Viscum coloratum plants in Yichun, China. The infected leaves were chlorotic, while sunken lesions formed on diseased branches, which were initially light brown and later turned dark brown. Moreover, diseased branches and leaves formed semi-buried, small, and spherical pycnidia. In total, 20 leaves and 19 branches diseased samples from Yichun, China were collected and examined. The fungus was isolated and identified using Koch’s postulates, morphological characteristics and DNA sequence data. The data presented herein confirmed that the pathogen responsible for the disease was Sphaeropsis visci. To the best of our knowledge, this is the first report of it in China.
KeywordsBotryosphaeriales Viscum coloratum Morphology Phylogeny Sphaeropsis visci New pathogen
We thank all my colleagues who helped in writing this thesis.
- Calder M, Bernhardt P (1983) The biology of mistletoes. Academic Press, Sydney, p 348Google Scholar
- Chai RY, Jin MZ (1991) Describe a method for direct selection of monospora to obtain monospora strains. Plant Prot 3:53–54Google Scholar
- Grover DW, Nicole JM (1940) The vapour pressure of glycerin solutions at twenty degrees. J Soc Chem Ind 59:175–177Google Scholar
- McNeill J (2012) International code of nomenclature for algae, fungi and plants (Melbourne code): adopted by the Eighteenth International Botanical Congress Melbourne, Australia, July 2011, vol 154. Koeltz Scientific Books, Königstein, pp 1–240Google Scholar
- Qiu HX (1988) Flora of China: volume 24: Mistletoe. Science Press, Beijing, pp 148–149Google Scholar
- Stojanović S (1989) The investigation of Shaeropsis visci (Salm.) Sacc Colletotrichum gloeosporoides (Sacc.) Penz, parasite on European (Viscum album ssp. typicum Beck). Zaštita Bilja 40:493–503Google Scholar
- Sutton BC (1980) The Coelomycetes: fungi imperfecti with pycnidia, acervuli, and stromata. Kew, Surrey, Commonwealth Mycological Institute, p 696Google Scholar
- White TJ, Bruns T, Lee S (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA (ed) PCR protocol: a guide to methods and applications. Academic Press, New York, pp 315–322Google Scholar