Morphological phylogeny among 15 accessions of bamboos growing in Kokrajhar district of the Bodoland Territorial Area Districts, Assam

  • Anusriti Basumatary
  • Sushil Kumar Middha
  • Talambedu Usha
  • Samik Bhattacharya
  • Birendra Kumar Brahma
  • Arvind Kumar Goyal
Original Paper


Bamboo is nature’s wonderful gift historically associated with the rural population of the Bodoland Territorial Area Districts. However, indiscriminate use of the resource and the ambiguity in the identification of bamboos in the absence of flowering characters pose a potential risk to the bamboo germplasm. Therefore, we considered revising the taxonomic positions and phylogenetic relationships among 15 tropical bamboo species using 35 key morphological descriptors (MD) to assess the reliability of vegetative characters in identifying species. The phylogenetic tree constructed from the similarity matrix, derived from an unweighted pair group method of analysis (UPGMA) of the 35 MDs, marginally deviated from the conventional taxonomic positions of the bamboo species. While the two varieties, one forma of Bambusa vulgaris (var. Vittata, var. Vulgaris, fo. Waminii) and two species of the genus Dendrocalamus (D. giganteus and D. hamiltonii) clustered together in a single clade, and D. strictus was distantly placed from their congeners. This discrepancy with the conventional classification might have ensued since only vegetative characters such as culm anatomy, culm sheath, leaf, and leaf sheath were considered in the present study. While the phylogenetic relationship among the 15 tropical bamboos (computed from exhaustive MDs) grossly corroborate the conventional taxonomic grouping, a molecular-marker-based multidisciplinary approach should resolve the remaining conflicts in bamboo identification.


Bamboo Bodoland Culm Culm-sheath Leaf-sheath Phylogenetic relationship conflict 

Supplementary material

11676_2017_535_MOESM1_ESM.docx (43 kb)
Supplementary material 1 (DOCX 42 kb)


  1. Bahadur KN, Jain SS (1983) Rare bamboos of India. In: Jain SK, Rao RR (eds) An assessment of threatened plants of India. Howrah West Bengal India Botanical survey of India Publication, Howrah, pp 265–271Google Scholar
  2. Barooah C, Borthakur SK (2003) Diversity and distribution of Bamboos in Assam. Bishen Singh B, Singh MP Publishers and Distributors of Scientific Books, Dehra Dun, p 17Google Scholar
  3. Basumatary A, Middha SK, Usha T, Brahma BK, Goyal AK (2015) Bamboo, as potential sources of food security, economic prosperity and ecological security in North-East India: an overview. Res Plant Biol 5(2):17–23Google Scholar
  4. Biswas SC (1988) Studies on bamboo distribution in North Eastern region of India. Indian For 114(9):514–531Google Scholar
  5. Brahma BK, Basumatary A, Basumatary J, Narzary D, Mwshahary N, Jamatia S, Basumatary P, Goyal AK (2014) Inventorying bamboo diversity of Kokrajhar district, BTAD, Assam, India with emphasis on its uses by the Bodos and allied tribes. Int J Fund Appl Sci 3(3):30–34Google Scholar
  6. Brown-Guedira GL, Thompson JA, Nelson RL, Warburton ML (2000) Evaluation of genetic diversity of soybean introductions and North American ancestors using RAPD and SSR markers. Crop Sci 40:815–823CrossRefGoogle Scholar
  7. Clayton WD, Vorontsova MS, Harman HK, Williamson H (2002) World grass species: descriptions, identification, and information retrieval. Royal Botanic Gardens, Kew.
  8. Das M, Bhattacharya S, Basak J, Pal A (2007) Phylogenetic relationships among the bamboo species as revealed by morphological characters and polymorphism analyses. Biol Plant 51(4):667–672CrossRefGoogle Scholar
  9. Das M, Bhattacharya S, Singh P, Filgueira TS, Pal A (2008) Bamboo taxonomy and diversity in the era of molecular markers. Adv Bot Res 47:225–268CrossRefGoogle Scholar
  10. Gamble JS (1896) The Bambuseae of British India. Ann Roy Bot Garden, Calcutta 7(1):1–133Google Scholar
  11. Ghosh S, Waikhom SD, SenMandi S, Talukdar NC (2011) Amplified Fragment Length Polymorphism based study of phylogenetic relationship and genetic variability among some edible bamboo species of North-East India. J Plant Mol Biol Biotechnol 2(2):8–15Google Scholar
  12. Ghosh S, Somkuwar B, Mandi SS, Talukdar NC (2012) Genetic variability and phylogenetic relationship among some bamboo species of North-East India by AFLP analysis. Asian J Plant Sci Res 2(4):478–485Google Scholar
  13. Goyal AK, Brahma BK (2014) Antioxidant and nutraceutical potential of Bamboo: an overview. Int J Fund Appl Sci 3(1):2–10Google Scholar
  14. Goyal AK, Sen A (2015) Phylogenetic relationships among accessions of bamboos encountered in North Bengal, India based on RAPD and ISSR markers. Indian J Biotechnol 14(1):495–503Google Scholar
  15. Goyal AK, Sen A (2016) In vitro regeneration of bamboos, the “Green Gold”: an overview. Indian J Biotechnol 15(1):9–16Google Scholar
  16. Goyal AK, Ghosh PK, Dubey AK, Sen A (2012) Inventorying bamboo biodiversity of North Bengal: A case study. Int J Fund Appl Sci 1:5–8Google Scholar
  17. Goyal AK, Kar P, Sen A (2014) Advancement of bamboo taxonomy in the era of molecular biology: a review. In: Sen A (ed) Biology of useful plants and microbes. Narosa Publishing House, New Delhi, pp 197–208Google Scholar
  18. Hore DK (1998) Genetic resources among bamboos of Northeastern India. J Econ Taxon Bot 22(1):173–181Google Scholar
  19. Loh JP, Kiew R, Set O, Gan LH, Gan YY (2000) A study of genetic variation and relationship within the bamboo subtribe Bambusinae using amplified fragment length polymorphism. Ann Bot 85:607–612CrossRefGoogle Scholar
  20. Mukherjee AK, Ratha S, Dhar S, Debata AK, Acharya PK, Manda S, Panda PC, Mahapatra AK (2010) Genetic relationships among 22 taxa of bamboo revealed by ISSR and EST based random primers. Biochem Genet 48:1015–1025CrossRefPubMedGoogle Scholar
  21. Nayak S, Rout GR, Das P (2003) Evaluation of the genetic variability in bamboo using RAPD markers. Plant Soil Environ 49:24–28Google Scholar
  22. Nei M, Li W (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 79:5269–5273CrossRefGoogle Scholar
  23. Ni J, Colowit PM, Mackill DJ (2002) Evaluation of genetic diversity in rice subspecies using microsatellite markers. Crop Sci 42:601–607CrossRefGoogle Scholar
  24. Ramanayake SMSD, Meemaduma VN, Weerawardene TE (2007) Genetic diversity and relationships between nine species of bamboo in Sri Lanka, using random amplified polymorphic DNA. Plant Syst Evol 269:55–61CrossRefGoogle Scholar
  25. Rohlf FJ (2000) NTSYS-pc: Numerical taxonomy and multivariate analysis system (2.2 Version). Exeter Software Setauket, NewYorkGoogle Scholar
  26. Sharma H, Sarma AM, Sarma A, Borah S (2010) A case of gregarious flowering in Bamboo, dominated low-land forest of Assam, India: phenology, regeneration, impact on rural economy and conservation. J For Res 21(4):409–414CrossRefGoogle Scholar
  27. Sneath PHA, Sokal RR (1973) Numerical taxonomy: the principles and practice of numerical classification. Freeman Publishing house, San Francisco, p 573Google Scholar
  28. Soderstrom TR, Ellis RP (1988) The woody bamboos (Poaceae: Bambusoideae) of Sri Lanka: a morphological anatomical study. Smithson Contrib Bot 72:1–75CrossRefGoogle Scholar
  29. Sungkaew S, Stapleton CM, Salamin N, Hodkinson TR (2009) Non-monophyly of the woody bamboos (Bambuseae; Poaceae): a multi-gene region phylogenetic analysis of Bambusoideae ss. J Plant Res 122(1):95–108CrossRefPubMedGoogle Scholar
  30. Tamang DK, Dhakal D, Gurung S, Sharma NP, Shreshtha DG (2013) Bamboo diversity distribution pattern and its uses in Sikkim (India) Himalaya. Int J Sci Res Publ 3(2):1–6Google Scholar
  31. Vengala J, Lindt JW (2013) Seismic performance of single family dwellings constructed using bamboo-mortal composite. Asian J Civil Eng 14(1):33–45Google Scholar
  32. Xia J, Wishart DS (2016) Using MetaboAnalyst 3.0 for comprehensive metabolomics data analysis. Curr Protoc Bioinform 55:14.10.1–14.10.91CrossRefGoogle Scholar
  33. Yeasmin L, Ali MN, Gantait S, Chakraborty S (2015) Bamboo: an overview on its genetic diversity and characterization. 3 Biotech 5(1):1–11CrossRefPubMedGoogle Scholar

Copyright information

© Northeast Forestry University and Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Anusriti Basumatary
    • 1
  • Sushil Kumar Middha
    • 2
  • Talambedu Usha
    • 3
  • Samik Bhattacharya
    • 4
  • Birendra Kumar Brahma
    • 1
  • Arvind Kumar Goyal
    • 1
  1. 1.Centre for Bamboo Studies and Department of BiotechnologyBodoland UniversityKokrajhar, BTADIndia
  2. 2.DBT-BIF Centre, Department of Biotechnology, PG Research CentreMaharani Lakshmi Ammanni College for WomenBengaluruIndia
  3. 3.Department of BiochemistryMaharani Lakshmi Ammanni College for WomenBengaluruIndia
  4. 4.Department of Molecular EcologyMax Planck Institute of Chemical EcologyJenaGermany

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