Journal of Forestry Research

, Volume 28, Issue 3, pp 453–463

Two novel eukaryotic translation initiation factor 5A genes from Populus simonii × P. nigra confer tolerance to abiotic stresses in Saccharomyces cerevisiae

Original Paper

Abstract

The role of plant eIF5A proteins in multiple biological processes, such as protein synthesis regulation, translation elongation, mRNA turnover, programmed cell death and stress tolerance is well known. Toward using these powerful proteins to increase stress tolerance in agricultural plants, in the present study, we cloned and characterized PsneIF5A2 and PsneIF5A4 from young poplar (P. simonii × P. nigra) leaves. The deduced amino acid sequences of PsneIF5A2 and PsneIF5A4 were 98 % similar to each other, and they are orthologs of eIF5A1 in Arabidopsis. In a subcellular localization analysis, PsneIF5A2 and PsneIF5A4 proteins were localized in the nucleus and cytoplasm. qRT-PCR analysis showed that PsneIF5A2 and PsneIF5A4 were transcribed in poplar flowers, stem, leaves, and roots. In addition, they were also induced by abiotic stresses. Transgenic yeast expressing PsneIF5A2 and PsneIF5A4 had increased salt, heavy metal, osmotic, oxidative tolerance. Our results suggest that PsneIF5A2 and PsneIF5A4 are excellent candidates for genetic engineering to improve salt and heavy metal tolerance in agricultural plants.

Keywords

Abiotic tolerance eIF5A Populus simonii × P. nigra Subcellular localization Yeast 

Abbreviations

ABA

Abscisic acid

eIF5A

Eukaryotic translation initiation factor 5A

EV

Empty vector

Gal

Galactose

Glu

Glucose

NLS

Nuclear localization signal

ORF

Open reading frame

qRT-PCR

Quantitative real time polymerase chain reaction

RT-PCR

Reverse transcriptase polymerase chain reaction

SC

Synthetic complete medium

Ura

Uracil

Copyright information

© Northeast Forestry University and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.State Key Laboratory of Tree Genetics and BreedingNortheast Forestry UniversityHarbinChina

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