Journal of Forestry Research

, Volume 27, Issue 2, pp 239–245 | Cite as

Identification of ISSR markers linked to flowering traits in a representative sample of Eucalyptus cladocalyx

  • Rodrigo Contreras-Soto
  • Paulina Ballesta
  • Eduardo Ruiz
  • Freddy Mora


Early flowering and flower abundance have long been considered desirable traits in eucalypt breeding programs. In particular, flowers of Eucalyptus cladocalyx provide a nectar source for the production of honey in arid ecosystems. To identify inter-simple sequence repeat (ISSR) markers that are associated with early flowering and flower abundance in the southern Atacama Desert, we used a sample of 47 trees, representing five Australian provenances of E. cladocalyx. A unified mixed linear model (which considered the effect of genetic structure and the kinship relationship among trees) revealed that three loci were significantly associated with early flowering, which accounted for 10–16 % of the phenotypic variation, while two loci accounted for 11–13 % in flowering intensity. Locus ISO1–500 bp was associated with both flowering traits. This result is consistent with our previous findings indicating that marker-assisted selection on early flowering should have significant and positive impact on flowering intensity. The application of marker-assisted selection to identify trees that flower early and intensively may increase honey production, a resource that generates additional income for the local farmers of the southern Atacama Desert.


Eucalyptus cladocalyx ISSR Flowering traits Marker-assisted selection 



This study was supported by the Chilean National Science and Technology Research Fund (FONDECYT); projects 11090129 and 1130306. We thank Mr. Augusto Gomes, the farmer who allowed the trial to take place on their property.


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Copyright information

© Northeast Forestry University and Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Rodrigo Contreras-Soto
    • 1
  • Paulina Ballesta
    • 2
  • Eduardo Ruiz
    • 2
  • Freddy Mora
    • 3
  1. 1.Department of AgronomyState University of MaringáMaringáBrazil
  2. 2.Department of BotanyUniversity of ConcepciónConcepciónChile
  3. 3.Institute of Biological SciencesUniversity of TalcaTalcaChile

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