Journal of Forestry Research

, Volume 26, Issue 1, pp 107–114 | Cite as

Genetic diversity analysis of Lepidium sativum (Chandrasur) using inter simple sequence repeat (ISSR) markers

  • Amandeep Kaur
  • Rakesh Kumar
  • Suman Rani
  • Anita Grewal
ORIGINAL PAPER
First Online:
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Accepted:

Abstract

Lepidium sativum (commonly known as garden cress) belongs to the family Brassicaceae. It is a fast-growing erect, annual herbaceous plant. Its seeds possess significant fracture healing, anti-asthmatic, anti-diabetic, hypoglycemic, nephrocurative and nephroprotective activities. In the present study, we assessed the genetic diversity of various genotypes of L. sativum using inter-simple sequence repeat (ISSR) markers. Out of 41 ISSR primers screened, 32 primers showed significant, clear and reproducible bands. A total of 510 amplified bands were obtained using 32 ISSR primers, out of which 422 bands were polymorphic and 88 bands were monomorphic. The percentage of polymorphism was found to be 82. A total of 35 unique alleles ranging insize from 200 to 2,900 bp were observed. Cluster analysis based on unweighted pair-group method, arithmetic mean divided the 18 genotypes into two main clusters, with the first having only HCS-08 genotype of L. sativum and other having all of the other 17 genotypes. The Jaccard similarity coefficient revealed a broad range 32–72 % genetic relatedness among the 18 genotypes.

Keywords

ISSR Genetic diversity Polymorphism Lepidium sativum Cluster analysis 
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Copyright information

© Northeast Forestry University and Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Amandeep Kaur
    • 1
  • Rakesh Kumar
    • 1
  • Suman Rani
    • 1
  • Anita Grewal
    • 1
  1. 1.Department of Biotechnology, University Institute of Engineering & TechnologyKurukshetra UniversityKurukshetraIndia

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