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Journal of Forestry Research

, 20:372 | Cite as

Activities of lipoxygenase and phenylalanine ammonia lyase in poplar leaves induced by insect herbivory and volatiles

  • Zeng-hui Hu
  • Wen Zhang
  • Ying-bai Shen
  • Huai-jun Fu
  • Xiao-hua Su
  • Zhi-yi Zhang
Research Paper

Abstract

A study was conducted to explore the defense response in woody plants after insect herbivory. The activities of two enzymes, lipoxygenase (LOX), a key enzyme of jasmonate (JA) pathway, and phenylalanine ammonia lyase (PAL), a rate-limiting enzyme of phenylpropanoid pathway, were measured in the leaves of one-year-old poplar (Populus simonii × P. pyramidalis ‘Opera 8277’) cuttings after Clostera anachoreta larvae attack. The results show that the increased activities of LOX and PAL were found not only in the leaves wounded by C. anachoreta larvae but also in their upper systemic leaves, indicating that JA and phenylpropanoid pathways were activated, and the defense response was stimulated systemically. The increase in LOX and PAL activities in neighboring intact poplar cuttings suggested that there exists the interplant communication between poplar plants mediated by the herbivore-induced volatiles. Methyl jasmonate (MeJA) was also proved to be an airborne signal to induce defense response in P. simonii × P. pyramidalis ‘Opera 8277’ cuttings.

Keywords

Clostera anachoreta larvae lipoxygenase methyl jasmonate phenylalanine ammonia lyase Populus simonii × P. pyramidalis ‘Opera 8277’ cuttings 

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Copyright information

© Northeast Forestry University and Springer Berlin Heidelberg 2009

Authors and Affiliations

  • Zeng-hui Hu
    • 1
    • 2
  • Wen Zhang
    • 3
  • Ying-bai Shen
    • 1
  • Huai-jun Fu
    • 4
  • Xiao-hua Su
    • 5
  • Zhi-yi Zhang
    • 1
  1. 1.Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants (Ministry of Education), College of Biological Sciences and TechnologyBeijing Forestry UniversityBeijingP. R. China
  2. 2.Department of GardeningBeijing University of AgricultureBeijingP. R. China
  3. 3.Department of ChemistryTsinghua UniversityBeijingP. R. China
  4. 4.Beijing Botanical GardenBeijingP. R. China
  5. 5.Research Institute of ForestryChinese Academy of ForestryBeijingP. R. China

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