Chinese Journal of Cancer Research

, Volume 23, Issue 1, pp 43–48

Factors affecting mesenchymal stromal cells yield from bone marrow aspiration

  • Jing Li
  • Wilfred Hing-Sang Wong
  • Shing Chan
  • James Chor-San Chim
  • Kenneth Man-Chee Cheung
  • Tsz-Leung Lee
  • Wing-Yan Au
  • Shau-Yin Ha
  • Albert Kwok-Wei Lie
  • Yu-Lung Lau
  • Raymond Hin-Suen Liang
  • Godfrey Chi-Fung Chan
Original Article

Abstract

Objective

This study was to investigate the variables in bone marrow harvesting procedure and individual donor factors which can potentially affect the yield of mesenchymal stromal cells (MSC).

Methods

We determined the yield of MSC from bone marrow under different clinical conditions by comparing the MSC colony numbers from: (1) donors of different ages; (2) healthy donors and patients with leukemia; (3) bone marrow aspirated at different time points during marrow harvesting; (4) bone marrow harvested by different needles.

Results

During the process of harvesting, the number of MSC significantly decreased with increase number of aspiration, from 675/ml at the initial decreased to 60/ml after 100 ml bone marrow aspirated, and 50/ml after 200 ml bone marrow aspirated. The number of MSC retrieved from leukemia patients (99/ml bone marrow) was significantly lower than that of healthy donors (708/ml bone marrow). However, there was no significant difference in growth rate. There was no significant age-related difference of MSC yielded from donors <55 years. And there was no significant difference in MSC number between the samples from single end-holed needle and those from multiple-side-hole needle.

Conclusion

The optimal bone marrow samples for MSC collection should be obtained earlier in the process of harvesting procedure. Bone marrow from donors <55 years was equally good as MSC sources. The autologous MSC from leukemia patients can be utilized for in-vitro MSC expansion.

Key words

Mesenchymal stromal cell CFU-F Bone marrow aspiration Leukemia 

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Copyright information

© Chinese Anti-Cancer Association and Springer Berlin Heidelberg 2011

Authors and Affiliations

  • Jing Li
    • 1
    • 2
  • Wilfred Hing-Sang Wong
    • 2
  • Shing Chan
    • 2
  • James Chor-San Chim
    • 3
  • Kenneth Man-Chee Cheung
    • 4
  • Tsz-Leung Lee
    • 2
  • Wing-Yan Au
    • 3
  • Shau-Yin Ha
    • 2
  • Albert Kwok-Wei Lie
    • 3
  • Yu-Lung Lau
    • 2
  • Raymond Hin-Suen Liang
    • 3
  • Godfrey Chi-Fung Chan
    • 2
  1. 1.China Oxford Centre for International Health Research, Cardiovascular Institute & Fuwai HospitalChinese Academy of Medical Sciences & Peking Union Medical CollegeBeijingChina
  2. 2.Department of Paediatrics and Adolescent Medicinethe University of Hong Kong, HKSARHong KongChina
  3. 3.Department of Medicinethe University of Hong Kong, HKSARHong KongChina
  4. 4.Department of Orthopaedics & Traumatologythe University of Hong Kong, HKSARHong KongChina

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