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Detection of human papilloma virus type 16 E6 mRNA in laryngeal squamous cell carcinoma by in situ hybridization

  • Original Article
  • Published:
Chinese Journal of Cancer Research

Abstract

Objective

Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumor in Northeast China and is frequently associated with well-established risk factors like smoking and alcohol abuse. Human papilloma virus (HPV) is an epitheliotropic oncogenic virus that has been detected in a variety of head and neck tumors including LSCC. This retrospective study was to investigate the prevalence of HPV infection in patients with LSCC.

Methods

In situ hybridization was performed in 99 patients with LSCC to detect the expression of HPV-16 E6 mRNA.

Results

The positive rate of HPV16 E6 mRNA was 36.36% (36/99) in laryngeal squamous cell carcinoma (LSCC), whereas only 3 of 50 (6%) specimens of the normal laryngeal mucosa as a control group showed positive results (P<0.05).Additionally, there was no corelation between HPV16 and age, gender, clinical stage, nodal status and tumor site (P>0.05).

Conclusion

The results suggest that the increased prevalence of HPV infection compared to normal laryngeal mucosa and the fact that high-risk HPV types (especially type 16) were the most frequently identified do not allow the exclusion of HPV as a risk factor in laryngeal squamous cell carcinoma. However, their clinical value remains to be further investigated.

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Authors and Affiliations

  1. Department of Clinical Laboratory, Chongqing Medical University, Chongqing, 630001, China

    Jiang Hai-rong

  2. Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Head and Neck Surgery, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing, 100142, China

    Wang Peng & Liu Bao-guo

  3. Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Genetics, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing, 100142, China

    Li Yong & Ning Tao

  4. Department of Pathology, Peking University Shougang Hospital, Beijing, 100144, China

    Rao Xiao-song

Authors
  1. Jiang Hai-rong
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  2. Wang Peng
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  3. Li Yong
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  4. Ning Tao
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  5. Rao Xiao-song
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  6. Liu Bao-guo
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Correspondence to Liu Bao-guo.

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Contributed equally to this study.

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Hai-rong, J., Peng, W., Yong, L. et al. Detection of human papilloma virus type 16 E6 mRNA in laryngeal squamous cell carcinoma by in situ hybridization. Chin. J. Cancer Res. 22, 218–223 (2010). https://doi.org/10.1007/s11670-010-0218-1

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  • DOI: https://doi.org/10.1007/s11670-010-0218-1

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