Chinese Journal of Cancer Research

, Volume 22, Issue 2, pp 141–147

Triptolide inhibits cell growth and induces G0- G1 arrest by regulating P21wap1/cip1 and P27 kip1 in human multiple myeloma RPMI-8226 cells

  • Yuan Liu
  • Ling-lan Zeng
  • Yan Chen
  • Fei Zhao
  • Rui Li
  • Chun Zhang
  • Lu Wen
Original Article

Abstract

Objective

To investigate the effects of triptolide(TPL) on cell growth, cell cycle and the expressions of p21wap1/cip1 and p27kip1.

Methods

MTT assay was used to determine the cell viability after triptolide treatment in human multiple myeloma RPMI-8226 cells. The effect on cell cycle distribution was determined by flow cytometry. Semi-quantitative reverse transcription-PCR was used to examine the mRNA expressions of p21wap1/cip1 and p27kip1. The protein expressions of p21 wap1/cip1 and p27kip1 were determined by Western blot.

Results

Triptolide of varying concentrations induced cell viability inhibition in dose- and time-related fashion and caused G0-G1 phase arrest of cell cycle progression in RPMI-8226 cells. These effects accompanied with up-modulation of the expressions of p21 wap1/cip1 and p27kip1.

Conclusion

These results suggest that triptolide inhibit cell proliferation and cell cycle progression via up-regulating p21wap1/cip1 and p27kip1 and triptolide may exert its anti-cancer activity through this pathway.

Key words

Triptolide RPMI-8226 cells Cell cycle Multiple myeloma 

CLC number

R733.3 

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Copyright information

© Chinese Anti-Cancer Association and Springer Berlin Heidelberg 2010

Authors and Affiliations

  • Yuan Liu
    • 1
  • Ling-lan Zeng
    • 1
  • Yan Chen
    • 1
  • Fei Zhao
    • 1
  • Rui Li
    • 1
  • Chun Zhang
    • 1
  • Lu Wen
    • 1
  1. 1.Institute of Hematology, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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