Chinese Journal of Cancer Research

, Volume 19, Issue 1, pp 1–6 | Cite as

Design of dendrimer modified carbon nanotubes for gene delivery

  • Pan Bi-feng  (潘碧峰)
  • Cui Da-xiang  (崔大祥)
  • Xu Ping  (徐萍)
  • Chen Hao  (陈浩)
  • Liu Feng-tao  (刘凤涛)
  • Li Qing  (李清)
  • Huang Tuo  (黄拓)
  • You Xiao-gang  (尤晓刚)
  • Shao Jun  (邵君)
  • Bao Chen-chen  (鲍晨晨)
  • Gao Feng  (高峰)
  • He Rong  (贺蓉)
  • Shu Meng-jun  (舒孟军)
  • Ma Yong-jie  (马勇杰)
Article

Abstract

Objective

To investigate the efficiency of polyamidoamine dendrimer grafted carbon nanotube (dendrimer-CNT) mediated entrance of anti-survivin oligonucleotide into MCF-7 cells, and its effects on the growth of MCF-7 cells.

Methods

Antisense survivin oligonucleotide was anchored onto polyamidoamine dendrimer grafted carbon nanotubes to form dendrimer-CNT-asODN complex and the complex was characterized by Zeta potential, AFM, TEM, and 1% agarose gel electrophoresis analysis. Dendrimer-CNT-asODN complexes were added into the medium and incubated with MCF-7 cells. MTT method was used to detect the effects of asODN and dendrimer-CNT-asODN on the growth of MCF-7 cells. TEM was used to observe the distribution of dendrimer-CNT-asODN complex within MCF-7 cells.

Results

Successful synthesis of dendrimer-CNT-asODN complexes was proved by TEM, AFM and agarose gel electrophoresis. TEM showed that the complexes were located in the cytoplasm, endosome, and lysosome within MCF-7 cells. When dendrimer-CNT-asODN (1.0 μmol/L) and asODN (1.0 μmol/L) were used for 120 h incubation, the inhibitory rates of MCF-7 cells were (28.22±3.5)% for dendrimer-CNT-asODN complex group, (9.23±0.56)% for only asODN group, and (3.44±0.25)% for dendrimer-CNT group. Dendrimer-CNT-asODN complex at 3.0 μmol/L inhibited MCF-7 cells by (30.30±10.62)%, and the inhibitory effects were in a time-and concentration-dependent manner.

Conclusion

Dendrimer-CNT nanoparticles may serve as a gene delivery vector with high efficiency, which can bring foreign gene into cancer cells, inhibiting cancer cell proliferation and markedly enhancing the cancer therapy effects.

Key words

Gene delivery Carbon nanotube Polyamidoamine dendrimer Cancer therapy Survivin gene 

CLC number

R73-36+

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Copyright information

© Chinese Anti-Cancer Association 2007

Authors and Affiliations

  • Pan Bi-feng  (潘碧峰)
    • 1
  • Cui Da-xiang  (崔大祥)
    • 1
  • Xu Ping  (徐萍)
    • 1
  • Chen Hao  (陈浩)
    • 1
  • Liu Feng-tao  (刘凤涛)
    • 1
  • Li Qing  (李清)
    • 1
  • Huang Tuo  (黄拓)
    • 1
  • You Xiao-gang  (尤晓刚)
    • 1
  • Shao Jun  (邵君)
    • 1
  • Bao Chen-chen  (鲍晨晨)
    • 1
  • Gao Feng  (高峰)
    • 1
  • He Rong  (贺蓉)
    • 1
  • Shu Meng-jun  (舒孟军)
    • 1
  • Ma Yong-jie  (马勇杰)
    • 1
  1. 1.Department of Bio-Nano Science and Engineering, Key Lab for Thin Film and Micro fabrication of Ministry of Education, State Key Lab of Micro-Nano Fabrication Technology, Institute of Micro-Nano Science and TechnologyShanghai Jiao Tong UniversityShanghaiChina

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