Chinese Journal of Cancer Research

, Volume 18, Issue 4, pp 241–245

A liver-tumor adhesion peptide from random phage display library

  • Wu Miao  (吴淼)
  • Du Bing  (杜冰)
  • Wang Lei  (汪磊)
  • Zhou Zhong-liang  (周忠良)
  • Qian Min  (钱旻)
Article

Abstract

Objective

To identify and localize the synthesized targeting peptide A54 to liver cancer cell line BEL-7502 in vivo and in vitro for confirming the potential clinical application of peptide A54 in hepatocarcinoma targeting therapy.

Methods

Phage A54 was confirmed by ELISA. Biotin and FAM labeled A54 peptides were identified and localized by means of immunohistochemistry and immunocytochemistry.

Results

A54 peptide could target the liver-tumor tissue in vivo and adhere to several liver-tumor cells in vitro. FAM-labeled A54 peptides were localized on the membrane surface of liver-tumor cells.

Conclusion

Synthesized A54 peptide obtained from in vivo phage display technology still kept special ability to adhere liver-tumor cell in vivo and in vitro. The A54 peptide could be a candidate carrier for hepatocarcinoma targeting therapy.

Key words

A54 peptide Identification localization Medicine carrier 

CLC number

R735.7 

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Copyright information

© Chinese Anti-Cancer Association 2006

Authors and Affiliations

  • Wu Miao  (吴淼)
    • 1
  • Du Bing  (杜冰)
    • 1
  • Wang Lei  (汪磊)
    • 1
  • Zhou Zhong-liang  (周忠良)
    • 1
  • Qian Min  (钱旻)
    • 1
  1. 1.School of Life ScienceEast China Normal UniversityShanghaiChina

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