Chinese Journal of Cancer Research

, Volume 17, Issue 2, pp 121–126 | Cite as

Detection of human papillomavirus L1 -16 and -18 DNA and epstein-barr virus DNA in laryngeal carcinoma

  • Liu Bao-guo 
  • Lu Zhe-ming 
  • Li Yong 
  • Jiang Hai-rong 
  • Liao Fei 
  • Peng Fang-yi 
  • Rao Xiao-song 
  • Chen Rong-rui 
  • Zhang Nai-song 
  • Wang Jian-jun 
  • Ke Yang 


Objective: To look for the further evidence for HPV L1 HPV16 E6, HPV 18 E6 and EBV as carcinogenic factors in laryngeal carcinoma. Method: we examined representative numbers of specimens from laryngeal cancer with highly sensitive PCR technique for the presence of HPV L1 and high-risk types HPV16 E6, HPV18 E6 and EBV LMP1. Results: Using PCR detection, 7.3% samples were HPV L1 positive, 52.03% were HPV16 E6 positive, 30.89% were HPV18 E6 positive and 9.13% were EBV LMP1 positive. The low incidence of HPV L1 and high incidence of HPV-16 E6 and HPV18 E6 genes suggest that HPV might be integrated into tumor cells. Our results support a role of HPV-16 and HPV-18 infection in the pathogenesis of laryngeal carcinoma in China. Conclusion: Integration of E6 into host genome and stable expression of these genes may be associated with the carcinogenesis of laryngeal carcinoma. HPV-16 and HPV-18 may synergistically function on the pathogenesis of laryngeal carcinoma. Our results suggest an association of laryngeal carcinogenesis and infection with the high-risk HPV types 16, HPV 18 and EBV.

Key words

EBV HPV Polymerase chain reaction Laryngeal carcinoma 

CLC number



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Copyright information

© Editorial Board of Chinese Journal of Cancer Research 2005

Authors and Affiliations

  • Liu Bao-guo 
    • 1
  • Lu Zhe-ming 
    • 2
  • Li Yong 
    • 2
  • Jiang Hai-rong 
    • 3
  • Liao Fei 
    • 3
  • Peng Fang-yi 
    • 3
  • Rao Xiao-song 
    • 4
  • Chen Rong-rui 
    • 4
  • Zhang Nai-song 
    • 1
  • Wang Jian-jun 
    • 4
  • Ke Yang 
    • 2
  1. 1.Department of Head & Neck Surgery and Surgical OncologyPeking University School of Oncology, Beijing Institute for Cancer Research, Beijing Cancer HospitalBeijing
  2. 2.Department of GeneticsPeking University School of Oncology, Beijing Institute for Cancer Research, Beijing Cancer HospitalBeijing
  3. 3.Department of clinical laboratoryChongqing Medical University
  4. 4.Department of Head & Neck Surgery and Surgical OncologyPeking University Shougang HospitalBeijing

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