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Chinese Journal of Cancer Research

, Volume 17, Issue 4, pp 265–269 | Cite as

Effect of matrine on expression of HCCR1 and HCCR2 proteins in cultural human hepatocellular carcinomas cells

  • Geng Dong-jin Email author
  • Chen Jun-hao 
  • Chen Lei-lei 
  • Gu Xiang-fang 
  • Zhang Le 
  • Han Li 
  • Liu Yong 
Articles
  • 22 Downloads

Abstract

Objective: To explore the effects of matrine on HCCR1 and HCCR2 expression in cultural human hepatocellular carcinomas (HCC) cells at the level of gene and protein. Methods: Three methods, representational difference analysis (RDA) of cDNA, microarrays and fluorescence in situ hybridization (FISH) were used to detect levels of mRNA and protein expression of HCCR1 and HCCR-2 before and after treatment of matrine. Results: Matrine had inhibitory effect on the mRNA and protein expression of HCCR1 and HCCR2 in cultural HCC cells. Conclusion: Matrine has inhibitory effect on gene transcription, protein expression of HCCR1 and HCCR2 in cultural HCC cells.

Key words

Hepatocellular carcinomas cells Human HCCR1 and HCCR2 genes Matrine 

CLC number

R735.7 

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Copyright information

© Chinese Journal 2005

Authors and Affiliations

  • Geng Dong-jin 
    • 1
    Email author
  • Chen Jun-hao 
    • 1
  • Chen Lei-lei 
    • 1
  • Gu Xiang-fang 
    • 1
  • Zhang Le 
    • 1
  • Han Li 
    • 1
  • Liu Yong 
    • 1
  1. 1.Molecular Medicine Center and Medical Laboratory Center, Affiliated Drum Tower HospitalNanjing UniversityNanjing

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