Chinese Journal of Cancer Research

, Volume 14, Issue 2, pp 84–87 | Cite as

Mycoplasma hyorhinis in gastric cancer

  • Ji Jia-fu 
  • Zhang Ji 
  • Shou Cheng-chao 
  • Wang Yi 
  • Xu Guang-wei 


Objective: To determine the prevalence of Mycoplasma hyorhinis in archived paraffin-embedded gastric cancer tissue. Methods: The antigen recognized by anti-tumor monoclonal antibody PD4 was identified as P40 (a specific Mycoplasma hyorhinis protein). We constructed a tissue-microarray of high density containing 105 gastric cancer samples, 101 non-tumor margin samples and 62 benign gastric disease samples for detecting Mycoplasma hyorhinis using Immunohistochemistry. Results: The infection rate of M. hyorhinis was 54.1% (53/98) in gastric cancer samples, 51.7%(45/87) in non-tumor margin samples and 15.8% (9/57) in benign disease samples. The difference in infection rate between gastric cancer and benign gastric disease has statistical significance (P=0.001). Highly differentiated adenocarcinomas have a greater chance (84.6%) to be infected with M. hyorhinis than poorly differentiated ones (45.5%) (P<0.05. Conclusion: The infection rate of M. yorhinis was higher in gastric cancer than in other tastric diseases, which suggests the association between Mycoplasma infection and gastric cancer. Whether M. hyorhinis has oncogenic potential needs to be elucidated.

Key words

Mycoplasma Gastric cancer Tissue microassay 

CLC number



Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    Kidder Melissa, Philip J, Chan. Assessment of archived paraffin-embedded cervical condyloma tissues for mycoplasma-conserved DNA using sensitive PCR-ELISA(J). Gynecol Oncol 1998; 71: 254.PubMedCrossRefGoogle Scholar
  2. [2]
    Gurr PA, Chakraverty A, Callanan V, et al. The detection of Mycoplasma pneumoniae in nasal polyps. Clin Otolaryngol 1996; 21: 269.PubMedGoogle Scholar
  3. [3]
    Sasaki Hiroki, Igaki Hiroyasu, et al. Presence of streptococcus DNA sequence in surgical specimens of gastric cancer. Jpn J Cancer Res 1995; 86: 791.PubMedGoogle Scholar
  4. [4]
    Philip J, Chan et al. Prevalence of mycoplasma conserved DNA in malignant ovarian cancer detected using sensitive PCR-ELISA(J). Gynecol Oncol 1996; 63: 258.CrossRefGoogle Scholar
  5. [5]
    Dong ZW, Wei SM, Mu ZY, et al. Monoclona antibodies against human gastric cancer. Chin J Cancer Res 1989; 1: 1.Google Scholar
  6. [6]
    Dong ZW, Yin WN, Deng GR, et al. P40 antigen mediating inhibitory effect on the proliferation of ras-transformed cells. J Exp Clin Cancer Res 1994; 13: 331.Google Scholar
  7. [7]
    Polianskaia GG, Efremova TN, Ender NA. Effedct of mycoplasma contamination of the human uterine leiomyosarcoma cell line SK-UT-1B on karyotype structure. Tsitologiia 1998; 40: 23.PubMedGoogle Scholar
  8. [8]
    Tsai S, DJ Wear, JWK Shih et al. Mycoplasma and oncogenesis: persistent infection and multistage malignant transformation. Proc Natl Acad Sci USA 1995; 92; 10197.PubMedCrossRefGoogle Scholar
  9. [9]
    Ushio S, Iwaki K, Taniai M, et al. Metastasis-promoting activity of a novel molecule, Ag243-5, derived from mycoplasma, and the complete nucleotide sequence. Microbiol Immunol 1995; 39: 393.PubMedGoogle Scholar

Copyright information

© the Editorial Board of Chinese Journal of Cancer Research 2002

Authors and Affiliations

  • Ji Jia-fu 
    • 1
  • Zhang Ji 
    • 1
  • Shou Cheng-chao 
    • 2
  • Wang Yi 
    • 1
  • Xu Guang-wei 
    • 1
  1. 1.Department of SurgeryPeking University School of Oncology, Beijing Cancer HospitalBeijing
  2. 2.Department of BiochemistryPeking University School of Oncology, Beijing Cancer HospitalBeijing

Personalised recommendations