Archives of Osteoporosis

, 8:147

Vitamin D receptor (VDR) gene polymorphism influences the risk of osteoporosis in postmenopausal women of Northwest India

  • Monica Singh
  • Puneetpal Singh
  • Surinder Singh
  • Pawan Kumar Juneja
  • Taranpal Kaur
Original Article

DOI: 10.1007/s11657-013-0147-y

Cite this article as:
Singh, M., Singh, P., Singh, S. et al. Arch Osteoporos (2013) 8: 147. doi:10.1007/s11657-013-0147-y

Abstract

Summary

The influence of VDR gene for the risk of osteoporosis has remained inconclusive. VDR gene polymorphism in relation to BMD in postmenopausal women of Northwest India revealed a susceptibility haplotype AGT. Possession of this haplotype exacerbates the risk of osteoporosis by 2.8 times, which manifests in recessive mode of inheritance.

Purpose

The purpose of this study is to understand the influence of coordinated effect of various single nucleotide polymorphisms (SNPs) within vitamin D receptor (VDR) gene for the risk of osteoporosis, which has remained undefined so far.

Methods

Four pertinent SNPs of VDR gene, i.e., rs2228570, rs1544410, rs17879735, and rs731236 were examined with polymerase chain reaction–restriction fragment length polymorphism in dual energy X-ray absorptiometry verified 188 osteoporotics, 115 osteopenics, and 147 normal postmenopausal women of Northwest India.

Results

Minor allele ‘T’ of rs2228570 showed significant influence for the risk of osteoporosis (OR 1.60, 95%CI 1.16–2.20, P = 0.004) and also in dominant (OR 2.32, 95%CI 1.47–3.64, P = 0.0006) and additive model (OR 2.41, 95%CI 1.49–3.87, P = 0.0006) after Bonferroni correction. Minor allele (T) of rs2228570 showed an allele dose effect with BMD of L1-L4 (P = 0.009) and FN (P = 0.036). Disease association analysis exposed a susceptibility haplotype AGT which influences the risk of osteopenia (OR 2.04, 95%CI 1.03–4.08, P = 0.036) and osteoporosis (OR 2.90, 95%CI 1.61–5.38, P = 0.00005) after adjusting the effects of age, BMI and years since menopause. This haplotype is significantly associated with BMDs at lumbar spine (P = 0.0001) and femoral neck (P = 0.016).

Conclusion

In-depth analysis of this haplotype with other methods of Wald statistics and Akaike information criterion confirmed that carriers of each unit of this haplotype AGT increases the risk of osteoporosis by a factor of 2.80 ± 0.34 (β ± SE) which manifests (P = 0.1 × 10−6) in its recessive mode of inheritance.

Keywords

VDR gene polymorphism Haplotypes PCR-RFLP BMD Osteoporosis India 

Supplementary material

11657_2013_147_MOESM1_ESM.docx (47 kb)
ESM 1(DOCX 47 kb)

Copyright information

© International Osteoporosis Foundation and National Osteoporosis Foundation 2013

Authors and Affiliations

  • Monica Singh
    • 1
  • Puneetpal Singh
    • 1
    • 4
  • Surinder Singh
    • 2
  • Pawan Kumar Juneja
    • 2
  • Taranpal Kaur
    • 3
  1. 1.Molecular Genetics Laboratory, Department of Human GeneticsPunjabi UniversityPatialaIndia
  2. 2.Aggarwal Orthopedic HospitalLudhianaIndia
  3. 3.Amrit Sagar HospitalFerozepurIndia
  4. 4.Department of Human GeneticsPunjabi UniversityPatialaIndia

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