Abstract
Objective
To compare the analgesic effects of two types of spinal manipulation (SM) in acute lumbar radiculopathy (ALR) model rats induced by self-transplantation of autologous nucleus pulposus (ANP), and clarify the therapeutic mechanism.
Methods
Totally 108 male Sprague-Dawley rats were randomly divided into 6 groups by a random number table (18 rats in each group), including a blank group with no interference, a sham operation group with a surgery by making a local soft tissue incision on the left side of L5–6 vertebral segment, a model group with ALR of L5 extraforaminal nerve by ANP self-transplantation without other interference, a sham manipulation (SMA) group with simulating physical rotation, as well as a mobilization (MOB) group with simulating low-velocity and variable-amplitude rotation and a manipulation (MAN) group with simulating high-velocity and low-amplitude rotation. The interventions in SMA, MOB, and MAN groups started 1 day after modeling followed by another 5 treatments at days 3, 5, 8, 10 and 12. Rats in the other 3 groups did not receive any special intervention. Behavioral pain tests of 50% mechanical pain withdrawal threshold (50% PWT) and paw withdrawal latency (PWL) were conducted 1 day before operation followed by another 10 tests on days 1–7, 10, 12 and 14. Immunohistochemical expression of nitric oxide synthase (NOS) was investigated on days 5 and 12 after operation.
Results
After 3 experimental SM interventions, 50% PWT and PWL were higher in the MAN group than the SMA group on days 6 and 7, and higher on days 10, 12 and 14 postoperatively (P<0.05 or P<0.01), while the same indices were significantly higher in the MOB group than MAN group on days 1–4 (P<0.05 or P<0.01). The expression of NOS was lower in the MAN and MOB groups than SMA group on day 12 postoperatively (P<0.01).
Conclusions
Both manipulation and mobilization produced better results than sham interference in relieving pain by reducing neuroinflammation possibly. At the early period, compared with manipulation, mobilization presented less sensitive response to pain until later visit. SM may inhibit the overexpression of NOS, thereby alleviating severe radiculopathy.
Similar content being viewed by others
References
Feng TY, ed. Clinical study on the treatment of soft tissue injury with integrated Chinese and Western medicine. Beijing: China Science and Technology Press; 2002:33–35.
Zhang MC, Zhan HS, Shi YY, Huang SY, Wang X. Diagnosis and treatment of intervertebral disc disease based on the theory of “Gucuofeng and Jinchucao”. Chin J Orthop Trauma (Chin) 2008;21:441–445.
Zhang MC, Zhan HS, Shi YY, Chen YW, Wang X, Huang SY. Systematic research on joint semi-dislocation and sinews offposition. Shanghai J Tradit Chin Med (Chin) 2009;43:59–63.
Zhu QG, Fang M, Pan L, Gu LX, Shen GQ, Chen YW, et al. Effects of tuina manipulation on the three-dimensional space of cervical vertebral segments of cervical spondylosis patients. Chin J Integr Tradit West Med (Chin) 2012;32:922–925.
Leong DJ, Sun HB. Mechanical loading: potential preventive and therapeutic strategy for osteoarthritis. J Am Acad Orthop Surg 2014;22:465–466.
Julita AT, Stephen I, Richard R. Spinal manipulative therapy reduces inflammatory cytokines but not substance P production in normal subjects. J Manipulat Physiol Ther 2006;29:14–21.
Sluka KA, Skyba DA, Radhakrihnan R, Leeper BJ, Wright A. Joint mobilization reduces hyperalgesia associated with chronic muscle and joint inflammation in rats. J Pain 2006;7:602–607.
Song XJ, Gan Q, Cao JL, Wang ZB, Rupert RL. Spinal manipulation reduces pain and hyperalgesia after lumbar intervertebral foramen inflammation in the rat. J Manip Physiol Ther 2006;29:5–13.
Parvaneh M, Antonio G, Chris T, Thomas H. Areas of capsaicin-induced secondary hyperalgesla and allodynia are deduced by a single chiropractic adjustment: a preliminary study. J Manip Physiol Ther 2004;27:381–387.
Onifer SM, Sozio RS, DiCarlo DM, Li Q, Donahue RR, Taylor BK, et al. Spinal manipulative therapy reduces peripheral neuropathic pain in the rat. Neuroreport 2018;29:191–196.
Brisby H. Pathology and possible mechanisms of nervous system response to disc degeneration. J Bone Joint Surg Am 2006;88(Suppl 2):68–71.
Tang JG, Yuan W, Hou SX, Wu WW, Shang WL. Roles of mechanical compression and non-compression in experimental radiculopathy. Chin J Spine Spinal Cord (Chin) 2006;16:865–868.
Fayad F, Lefevre-Colau MM, Rannou F, Quintero N, Nys A, Mace Y, et al. Relation of inflammatory modic changes to intradiscal steroid injection outcome in chronic low back pain. Eur Spine J 2007;16:925–931.
Takahashi N, Kikuchi S, Shubayev VI, Campana WM, Myers RR. TNF-alpha and phosphorylation of ERK in DRG and spinal cord: insight into mechanisms of sciatica. Spine 2006;31:523–259.
Zhao P. Recent study on the mechanism of Western spinal manipulation. Chin J Orthop Trauma (Chin) 2011;24:705–709.
Bronfort G, Haas M, Evans RL, Bouter LM. Efficacy of spinal manipulation and mobilization for low back pain and neck pain: a systematic review and best evidence synthesis. Spine J 2004;4:335–356.
Bronfort G, Haas M, Evans R, Kawchuk G, Dagenais S. Evidence-informed management of chronic low back pain with spinal manipulation and mobilization. Spine J 2008;8:213–225.
Han L, Zhao P, Guo W, Wei J, Li Y, Min YQ, et al. A rat model of sciatica induced by autologous nucleus pulposus implant at outlet of lumbar intervertebral foramen. Beijing J Tradit Chin Med (Chin) 2015;34:245–248.
Chaplan SR, Bach FW, Pogrel JW, Chung JM, Yaksh TL. Quantitative assessment of tactile allodynia in the rat paw. J Neurosci Methods 1994;53:55–63.
Dixon WJ. Efficient analysis of experimental observations. Annu Rev Pharmacol Toxicol 1980;20:441–462.
Hargreares K, Dubner R, Brown F, Flores C, Joris J. A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia. Pain 1988;32:77–88.
Vergnolle N, Cenac N, Altier C, Cellars L, Chapman K, Zamponi GW, et al. A role for transient receptor potential vanilloid 4 in tonicity-induced neurogenic inflammation. British J Pharmacol 2010;159:1161–1173.
Kawakami M, Tamaki T, Weinstein JN, Hashizume H, Nishi H, Meller ST. Pathomechanism of pain-related behavior produced by allografts of intervertebral disc in the rat. Spine 1996;21:2101–2107.
Sasaki N, Kikuchi S, Konno S, Sekiguchi M, Watanabe K. Anti-TNF-alpha antibody reduces pain-behavioral changes induced by epidural application of nucleus pulposus in a rat model depending on the timing of administration. Spine 2007;32:413–416.
Tang JG, Yuan W, Wang XW, Hou SX, Shang WL. Establishment of a new animal model of discogenic sciatic nerve pain. J Spin Surg 2008;6:144–147.
Wei M, Zhang JJ, He QL, Wang L, Ren ZH, Liu XG, et al. Establishment of a new model of sciatic neuralgia induced by lumbar intervertebral disc herniation and a method of epidural catheterization. Chin J Pain Med (Chin) 2011;17:744–748.
Brisby H, Byrod G, Olmarker K, Miller VM, Aoki Y, Rydevik B. Nitric oxide as a mediator of nucleus pulposus-induced effects on spinal nerve roots. J Orthop Res 2000;18:815–820.
Borsani E, Giovannozzi S, Cocchi MA, Boninsegna R, Rezzani R, Rodella LF. Endothelial nitric oxide synthase in dorsal root ganglia during chronic inflammatory nociception. Cells Tissue Org 2013;197:159–168.
Kawakami M, Matsumoto T, Hashizume H, Kuribayashi K, Tamaki T. Epidural injection of cyclooxygenase-2 inhibitor attenuates pain-related behavior following application of nucleus pulposus to the nerve root in the rat. Orthop Res 2002;20:376–381.
Author information
Authors and Affiliations
Contributions
Han L and Zhao P designed the study; Han L, Wei J, Wang F and Li Y performed the experiments and wrote the manuscript; Han X and Han L completed the modeling and collected the data; Guo GJ analyzed the data; Zhao P revised the manuscript. All authors read and agreed the final version for publication.
Corresponding author
Additional information
Conflict of Interest
All the authors declare that there is no conflict of interests regarding the publication of this paper.
Supported by Youth Project of National Natural Science Foundation of China (No. 81503602)
Rights and permissions
About this article
Cite this article
Han, L., Zhao, P., Han, X. et al. Analgesic Effects of Two Types of Spinal Manipulation in Acute Lumbar Radiculopathy Model Rats. Chin. J. Integr. Med. 28, 518–523 (2022). https://doi.org/10.1007/s11655-021-3276-y
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11655-021-3276-y