Combination of Korean Red Ginseng Extract and Hydrogen-Rich Water Improves Spermatogenesis and Sperm Motility in Male Mice

  • Ja Yoon Ku
  • Min Jung Park
  • Hyun Jun Park
  • Nam Cheol Park
  • Bo Sun JooEmail author
Original Article



To investigate the effect of hydrogen-rich Korean Red Ginseng (KRG) water (HRGW) mixture on the spermatogenesis and sperm motility of mice of different ages.


Eighty young (3 month-old) and aged (12 month-old) male mice were randomly assigned to 4 groups (n =10 per group) including control group, hydrogen-rich water (HRW) group (10 mL/kg daily), KRG group (50 mg/kg daily) and HRGW group (10 mL/kg and 50 mg/kg daily) by an oral zoned needle for 4 weeks. Sperm count and motility were measured using sperm suspension released from cauda epididymis. Serum follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone, and reactive oxygen species (ROS) in serum have also been estimated. Tubular changes were examined through histological hematoxylin and eosin staining. Expression of antioxidation (PPx3, PPx4, GSTm5 and GPx4), spermatogenesis (inhibin-a, neptin-2 and CREM), antiaging (SIRT1 and SIRT2), and angiogenesis [visfatin and vascular endothelial growth factor (VEGF)] related genes were examined through real-time polymerase chain reaction.


HRW and KRG treatment stimulated spermatogenesis followed by increasing sperm production and sperm motility (P <0.05). These effects were strengthened synergistically by a HRGW mixture (P <0.05 or P <0.01). HRGW greatly increased the expressions of antioxidation, antiaging, spermatogenesis related genes and VEGF especially in aged mice (P <0.05). Serum testosterone and FSH levels also increased, while serum ROS level decreased (all P <0.05).


HRGW increases sperm production and motility by enhancing antioxidation and stimulating spermatogenesis and sex hormone production, particularly in aged mice.


aging ginseng infertility mineral waters sperm motility 


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Author Contributions

Ku JY contributed to execution of study, acquisition of data, and manuscript drafting. Park MJ performed the study and statistical analysis; Park HJ and Park NC performed the data analysis and critical discussion. Joo BS contributed to conception and design of study, drafting the manuscript and revising it critically for important intellectual content, and approval for final version for publication.

Conflict of Interest

All authors have no conflicts of interest to declare.


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Copyright information

© The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Ja Yoon Ku
    • 1
    • 2
  • Min Jung Park
    • 3
  • Hyun Jun Park
    • 1
  • Nam Cheol Park
    • 1
    • 3
  • Bo Sun Joo
    • 3
    Email author
  1. 1.Department of UrologyPusan National University Hospital, Pusan National University School of MedicineBusanRepublic of Korea
  2. 2.Biomedical Research InstitutePusan National University HospitalBusanRepublic of Korea
  3. 3.The Korea Institute for Public Sperm BankBusanRepublic of Korea

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