Effect and Mechanism of Ganoderma lucidum Polysaccharides on Human Fibroblasts and Skin Wound Healing in Mice

  • Feng Hu
  • Yu Yan
  • Chu-wang Wang
  • Yu Liu
  • Jing-jing Wang
  • Fang Zhou
  • Qing-hai Zeng
  • Xiao Zhou
  • Jia Chen
  • Ai-jun Wang
  • Jian-da ZhouEmail author
Original Article



To investigate the effects of Ganoderma lucidum polysaccharides (GL-PS) on human fifi broblasts and skin wound healing in Kunming male mice and to explore the putative molecular mechanism.


Primary human skin fifi broblasts were cultured. The viability of fifi broblasts treated with 0, 10, 20, 40, 80, and 160 μg/mL of GL-PS, respectively were detected by 3–4,5-dimethyl-2-thiazolyl-2,5-diphenyl-2-Htetrazolium bromide (MTT). The migration ability of fifi broblasts treated with 0, 10, 20, and 40 μg/mL of GL-PS were measured by transwell assay. The secretion of the C-terminal peptide of procollagen type I (CICP) and transforming growth factor-β1 (TGF-β1) in the cell supernatant was tested by enzyme-linked immunosorbent assay. The expression of β-catenin was detected by Western blot. Furthermore, the Kunming mouse model with full-layer skin resection trauma was established, and was treated with 10, 20, and 40 mg/mL of GL-PS, respectively as external use. The size of the wound was measured daily, complete healing time in each group was recorded and the percentage of wound contraction was calculated.


Compared with the control group, 10, 20, and 40 μg/mL of GL-PS signififi cantly increased the viability of fifi broblasts, promoted the migration ability of fifi broblasts, and up-regulated the expressions of CICP and TGF-β1 in fifi broblasts (P<0.05 or P<0.01). The expression of β-catenin in fifi broblasts treated with 20 and 40 μg/mL of GL-PS was signififi cantly higher than that of the control group (P<0.01). Furthermore, after external use of 10, 20, and 40 mg/mL of GL-PS, the rates of wound healing in mice were signififi cantly higher and the wound healing time was signififi cantly less than the control group (P<0.05 or P<0.01).


A certain concentration of GL-PS may promote wound healing via activation of the Wnt/β-catenin signaling pathway and up-regulation of TGF-β1, which might serve as a promising source of skin wound healing.


Ganoderma lucidum polysaccharides human fibroblast wound healing transforming growth factor-β1 Wnt/β-catenin signaling pathway 


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Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine 2018

Authors and Affiliations

  • Feng Hu
    • 1
  • Yu Yan
    • 2
  • Chu-wang Wang
    • 1
  • Yu Liu
    • 1
  • Jing-jing Wang
    • 2
  • Fang Zhou
    • 2
  • Qing-hai Zeng
    • 1
  • Xiao Zhou
    • 3
  • Jia Chen
    • 1
  • Ai-jun Wang
    • 4
  • Jian-da Zhou
    • 1
    Email author
  1. 1.Department of Plastic Surgerythe Third Xiangya Hospital of Central South UniversityChangshaChina
  2. 2.Xiangya School of MedicineCentral South UniversityChangshaChina
  3. 3.Department of Head and Neck SurgeryHunan Province Cancer HospitalChangshaChina
  4. 4.Department of Surgery, Davis Health SystemUniversity of CaliforniaSacramentoUSA

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