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Preventive Effect and Mechanism of Ethyl Acetate Extract of Sceptridium ternatum in Monocrotaline-Induced Pulmonary Arterial Hypertension

  • Wen-xiu Xin
  • Qing-lin Li
  • Luo Fang
  • Li-ke Zhong
  • Xiao-wei Zheng
  • Ping HuangEmail author
Original Article
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Abstract

Objective

To observe the effect and molecular mechanism of ethyl acetate extract of Sceptridium ternatum (STE) on the monocrotaline (MCT)-induced pulmonary arterial hypertension (PAH).

Methods

The main chemical components of Sceptridium ternatum were determined, and the effects in PAH rats were observed. A total of 140 Sprague Dawley rats were randomly and equally divided into the normal group, the model group, the Bosentan group, and the STE groups (2.5, 5, 10 g/kg) by the random number table method. The characteristic indicators of PAH were measured, and immunohistochemistry was used to observe the lung tissue of rats. Morphological changes of the lung tissue were observed under the light microscope.

Results

Compared with the normal group, rats in the model group showed a signififi cant increase in right ventricular free wall thickness (RVFWT), mean pulmonary arterial pressure (mPAP), mean right ventricular pressure (mRVP), max right ventricular pressure (max RVP), weight of right ventricle (RV), and lung index (LI), while a signififi cant decrease in pulmonary artery acceleration time (PAAT, P<0.01). Compared with the model group, rats treated with STE had a signififi cant decrease of RVFWT, mPAP, mRVP, max RVP, and RV, while a signififi cant increase of PAAT (P<0.01). After injection of MCT, nuclear factor-κB (NF-κB) p65 and α-smooth muscle actin (α-SMA) expression levels were up-regulated, and on the contrary, the treatment groups showed a significant downregulation without dose-dependent trend.

Conclusions

STE can relieve the PAH in rats. STE may relieve pulmonary vascular disease and pulmonary injury by down-regulating the expression of NF-κB p65 and α-SMA.

Keywords

pulmonary arterial hypertension ethyl acetate extract of Sceptridium ternatum mechanism Chinese medicine 

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Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine 2018

Authors and Affiliations

  • Wen-xiu Xin
    • 1
  • Qing-lin Li
    • 1
  • Luo Fang
    • 1
  • Li-ke Zhong
    • 1
  • Xiao-wei Zheng
    • 1
  • Ping Huang
    • 1
    Email author
  1. 1.Department of PharmacyZhejiang Cancer HospitalHangzhouChina

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