Chinese Journal of Integrative Medicine

, Volume 25, Issue 10, pp 743–749 | Cite as

Genotoxicity and Embryotoxicity Study of Bicyclol Methyl Ether, Main Impurity in Bicyclol

  • Qian-qian Zhang
  • Qiang Li
  • Lin Dong
  • Wan-fang Li
  • Chao Li
  • Ai-ping Wang
  • Jin-feng Wei
  • Hong-tao JinEmail author
Original Article



To assess the genotoxicity and embryotoxicity of bicyclol methyl ether (BME), the main impurity in bicyclol.


Five concentrations of BME (0.5, 5, 50, 500 and 5000 μg/plate) were used in the Ames test to detect gene mutation. In the chromosome aberration test, Chinese hamster lung cells were used to detect chromosomal aberration of BME (15, 30, 60, 120 μg/mL) with or without S9 mixture. Embryotoxicity test was also conducted to determine any embryotoxicity of BME (7.5, 22.5, 67.5 μg/L) using zebrafish embryos.


No significant differences were observed in the Ames test and the chromosome aberration test in the BME groups compared with the vehicle control group. The zebrafish embryos toxicity test also showed no embryo development toxicity of BME, including hatching rate, body length, pericardial area and yolk sac area.


Bicyclol methyl ether has no genotoxicity in vitro and embryotoxicity in zebrafish embryos, and the impurity in bicyclol is qualified.


bicyclol methyl ether genotoxicity embryotoxicity Ames test chromosome aberration test zebrafish 


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Author Contributions

Zhang QQ, Dong L carried out the experiments and wrote the paper; Li Q, Li WF help sampling the test article and detection of HPLC; Li C, Wang AP, Wei JF supervised and directed the project; Jin HT as the corresponding author reviewed and revised the entire manuscript.

Supplementary material

11655_2018_2553_MOESM1_ESM.docx (18 kb)
Supplementary material, approximately 20 KB.


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Copyright information

© The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Qian-qian Zhang
    • 1
  • Qiang Li
    • 2
  • Lin Dong
    • 3
  • Wan-fang Li
    • 1
  • Chao Li
    • 3
  • Ai-ping Wang
    • 1
    • 3
  • Jin-feng Wei
    • 1
    • 3
  • Hong-tao Jin
    • 1
    • 3
    Email author
  1. 1.New Drug Safety Evaluation Center, Institute of Materia MedicaChinese Academy of Medical SciencesBeijingChina
  2. 2.Beijing Union Pharmaceutical FactoryBeijingChina
  3. 3.Beijing Union-Genious Pharmaceutical Technology Ltd.BeijingChina

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