Advertisement

Chinese Journal of Integrative Medicine

, Volume 24, Issue 9, pp 670–675 | Cite as

Suppression of Oxidative Stress of Modified Gongjin-Dan (WSY-1075) in Detrusor Underactivity Rat Model Bladder Outlet Induced by Obstruction

  • Jin-Woo Jung
  • Seung Hwan Jeon
  • Woong Jin Bae
  • Su Jin Kim
  • Mun Su Chung
  • Byung Il Yoon
  • Sae Woong Choi
  • U. Syn Ha
  • Sung Yeoun Hwang
  • Sae Woong KimEmail author
Original Article
  • 61 Downloads

Abstract

Objective

To investigate the anti-oxidative stress and preventive effect of modified Gongjin-dan (WSY-1075) in a detrusor underactivity rat model.

Methods

Rats were randomly allocated to three groups: shamoperated (control), bladder outlet obstruction-induced detrusor underactivity (BOO-DU), and BOO-DU with WSY-1075 (WSY) groups. WSY-1075 was orally administrated to rats 200 mg daily for 2 weeks prior to the operation and 4 weeks after the operation. Bladder outlet obstruction was surgically induced in rats by ligation around the urethra avoiding total obstruction. Cystometrography was conducted on rats in each group for examination of bladders.

Results

Compared with the control group, bladder outlet obstruction led to a significant increase in oxidative stress with consequent changes to molecular composition, and decrease in maximal detrusor pressure (P<0.05). WSY-1075 treatment significantly suppressed oxidative stress and prevented degenerative and dysfunctional changes in bladder, as compared with BOO-DU group (P<0.05).

Conclusion

WSY-1075 had beneficial effect on prevention of BOO-DU.

Keywords

detrusor underactivity anti-oxidant herbal 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Neveus T, von Gontard A, Hoebeke P, Hjalmas K, Bauer S, Bower W, et al. The standardization of terminology of lower urinary tract function in children and adolescents: report from the Standardisation Committee of the International Children’s Continence Society. J Urol 2006;176:314–324.CrossRefPubMedGoogle Scholar
  2. 2.
    Jeong SJ, Kim HJ, Lee YJ, Lee JK, Lee BK, Choo YM, et al. Prevalence and clinical features of detrusor underactivity among elderly with lower urinary tract symptoms: a comparison between men and women. Kor J Urol 2012;53:342–348.CrossRefGoogle Scholar
  3. 3.
    Osman NI, Chapple CR, Abrams P, Dmochowski R, Haab F, Nitti V, et al. Detrusor underactivity and the underactive bladder: a new clinical entity? A review of current terminology, definitions, epidemiology, aetiology, and diagnosis. Eur Urol 2014;65:389–398.PubMedGoogle Scholar
  4. 4.
    Kosilov K, Loparev S, Iwanowskaya M, Kosilova L. Effectiveness of combined high–dosed trospium and solifenacin depending on severity of OAB symptoms in elderly men and women under cyclic therapy. Cent Eur J Urol 2014;67:43–48.Google Scholar
  5. 5.
    Malki M, Mangera A, Reid S, Inman R, Chapple C. What is the feasibility of switching to 200IU onabotulinumtoxin A in patients with detrusor overactivity who have previously received 300IU? Cent Eur J Urol 2014;67:35–40.Google Scholar
  6. 6.
    Nishijima S, Sugaya K, Miyazato M, Kadekawa K, Oshiro Y, Uchida A, et al. Restoration of bladder contraction by bone marrow transplantation in rats with underactive bladder. Biomed Res 2007;28:275–280.CrossRefPubMedGoogle Scholar
  7. 7.
    Buttyan R, Chen MW, Levin RM. Animal models of bladder outlet obstruction and molecular insights into the basis for the development of bladder dysfunction. Eur Urol 1997;32:32–39.PubMedGoogle Scholar
  8. 8.
    Levin RM, Levin SS, Zhao Y, Buttyan R. Cellular and molecular aspects of bladder hypertrophy. Eur Urol 1997;32:15–21.PubMedGoogle Scholar
  9. 9.
    Bougas DA, Mitsogiannis IC, Mitropoulos DN, Kollaitis GC, Serafetinides EN, Giannopoulos AM. Clinical efficacy of distigmine bromide in the treatment of patients with underactive detrusor. Int Urol Nephrol 2004;36:507–512.CrossRefPubMedGoogle Scholar
  10. 10.
    Wyndaele JJ. Complications of intermittent catheterization: their prevention and treatment. Spinal Cord 2002;40:536–541.CrossRefPubMedGoogle Scholar
  11. 11.
    Levin RM, Kawashima Y, Leggett RE, Whitbeck C, Horan P, Mizutani K. Effect of oral Kohki tea on bladder dysfunction induced by severe partial outlet obstruction. J Urol 2002;167:2260–2266.CrossRefPubMedGoogle Scholar
  12. 12.
    Levin RM, Hass MA, Bellamy F, Horan P, Whitbeck K, Chow PH, et al. Effect of oral Tadenan treatment on rabbit bladder structure and function after partial outlet obstruction. J Urol 2002;167:2253–2259.CrossRefPubMedGoogle Scholar
  13. 13.
    Hong SS, Lee JY, Lee JS, Lee HW, Kim HG, Lee SK, et al. The traditional drug Gongjin–Dan ameliorates chronic fatigue in a forced–stress mouse exercise model. J Ethnopharmacol 2015;168:268–278.CrossRefPubMedGoogle Scholar
  14. 14.
    Bae WJ, Ha US, Kim S, Kim SJ, Hong SH, Lee JY, et al. Reduction of oxidative stress may play a role in the antiinflammatory effect of the novel herbal formulation in a rat model of hydrochloric acid–induced cystitis. Neurourol Urodyn 2015;34:86–91.CrossRefPubMedGoogle Scholar
  15. 15.
    Kim SJ, Park EY, Hwang TK, Kim JC. Therapeutic effects of connexin inhibitors on detrusor overactivity induced by bladder outlet obstruction in rats. Urology 2011;78:e471–e477.Google Scholar
  16. 16.
    Bae WJ, Ha US, Kim KS, Kim SJ, Cho HJ, Hong SH, et al. Effects of KH–204 on the expression of heat shock protein 70 and germ cell apoptosis in infertility rat models. BMC Complement Altern Med 2014;14:367.CrossRefPubMedPubMedCentralGoogle Scholar
  17. 17.
    Bae WJ, Ha US, Choi JB, Kim KS, Kim SJ, Cho HJ, et al. Protective effects of KH–204 in the bladder of androgendeprived rats. World J Mens Health 2015;33:73–80.CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Oka M, Fukui T, Ueda M, Tagaya M, Oyama T, Tanaka M. Suppression of bladder oxidative stress and inflammation by a phytotherapeutic agent in a rat model of partial bladder outlet obstruction. J Urol 2009;182:382–390.CrossRefPubMedGoogle Scholar
  19. 19.
    Juszczak K, Drewa T. Pharmacotherapy in detrusor underactivity: a new challenge for urologists and pharmacologists (from lab to clinic). Pharmacol Rep 2016;68:703–706.CrossRefPubMedGoogle Scholar
  20. 20.
    Zhao Z, Azad R, Yang JH, Siroky MB, Azadzoi KM. Progressive changes in detrusor function and micturition patterns with chroinc bladder ischemia. Investig Clin Urol 2016;57:249–259.CrossRefPubMedPubMedCentralGoogle Scholar
  21. 21.
    Jiang YH, Kuo HC. Urothelial barrier deficits, suburothelial inflammation and altered sensory protein expressions in detrusor underactivity. J Urol 2017;197:197–203.CrossRefPubMedGoogle Scholar
  22. 22.
    Tome LA, Yu L, de Castro I, Campos SB, Seguro AC. Beneficial and harmful effects of L–arginine on renal ischaemia. Nephrol Dial Transplant 1999;14:1139–1145.CrossRefPubMedGoogle Scholar
  23. 23.
    Conners W, Whitebeck C, Chicester P, Legget R, Lin AD, Johnson A, et al. L–NAME, a nitric oxide synthase inhibitor, diminishes oxidative damage in urinary bladder partial outlet obstruction. Am J Physiol Renal Physiol 2006;290:F357–F363.CrossRefPubMedGoogle Scholar
  24. 24.
    Whitbeck C, Chichester P, Sokol R, Levin RM. Role of nitric oxide in urinary bladder function: effect of L–arginine. Urol Int 2007;78:30–36.CrossRefPubMedGoogle Scholar
  25. 25.
    Kim SO, Choi D, Song SH, Ahn KY, Kwon D, Park K, et al. Effect of detrusor overactivity on the expression of aquaporins and nitric oxide synthase in rat urinary bladder following bladder outlet obstruction. Can Urol Assoc J 2013;7:268–274.CrossRefGoogle Scholar
  26. 26.
    Felsen D, Dardashti K, Ostad M, Lemer ML, Gross SS, Chen J, et al. Inducible nitric oxide synthase promotes pathophysiological consequences of experimental bladder outlet obstruction. J Urol 2003;169:1569–1572.CrossRefPubMedGoogle Scholar
  27. 27.
    Yuan X, Wu S, Lin T, He D, Li X, Liu S, et al. Role of nitric oxide synthase in bladder pathologic remodeling and dysfunction resulting from partial outlet obstruction. Urology 2011;77:e1001–e1008.CrossRefGoogle Scholar

Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine 2017

Authors and Affiliations

  • Jin-Woo Jung
    • 1
    • 2
  • Seung Hwan Jeon
    • 3
  • Woong Jin Bae
    • 3
    • 4
  • Su Jin Kim
    • 3
  • Mun Su Chung
    • 1
  • Byung Il Yoon
    • 1
  • Sae Woong Choi
    • 3
  • U. Syn Ha
    • 3
  • Sung Yeoun Hwang
    • 5
  • Sae Woong Kim
    • 3
    • 4
    Email author
  1. 1.Department of Urology, International St. Mary’s HospitalCatholic Kwandong University College of MedicineIncheonRepublic of Korea
  2. 2.Institute for Bio-Medical Convergence, College of MedicineCatholic Kwandong University, GangneungsiGangwon-doRepublic of Korea
  3. 3.Department of Urology, College of MedicineThe Catholic University of KoreaSeoulRepublic of Korea
  4. 4.Catholic Integrative Medicine Research Institute, College of MedicineThe Catholic University of KoreaSeoulRepublic of Korea
  5. 5.Korea Bio Medical Science InstituteSeoulRepublic of Korea

Personalised recommendations