Chinese Journal of Integrative Medicine

, Volume 23, Issue 2, pp 110–116 | Cite as

Brucine inhibits bone metastasis of breast cancer cells by suppressing Jagged1/Notch1 signaling pathways

  • Ke-fei Hu
  • Xiang-ying Kong
  • Mi-cun Zhong
  • Hong-ye Wan
  • Na Lin
  • Xiao-hua Pei
Original Article



To examine the effects of brucine on the invasion, migration and bone resorption of receptor activator of nuclear factor-kappa B ligand (RANKL)-induced osteoclastogenesis.


The osteoclastogenesis model was builded by co-culturing human breast tumor MDA-MB-231 and mouse RAW264.7 macrophages cells. RANKL (50 ng/mL) and macrophage-colony stimulating factor (50 ng/mL) were added to this system, followed by treatment with brucine (0.02, 0.04 and 0.08 mmol/L), or 10 μmol/L zoledronic acid as positive control. The migration and bone resorption were measured by transwell assay and in vitro bone resorption assay. The protein expressions of Jagged1 and Notch1 were investigated by Western blot. The expressions of transforming growth factor-β1 (TGF-β1), nuclear factor-kappa B (NF-κB) and Hes1 were determined by enzyme-linked immunosorbent assay.


Compared with the model group, brucine led to a dose-dependent decrease on migration of MDA-MB-231 cells, inhibited RANKL-induced osteoclastogenesis and bone resorption of RAW264.7 cells (P<0.01). Furthermore, brucine decreased the protein levels of Jagged1 and Notch1 in MDA-MB-231 cells and RAW264.7 cells co-cultured system as well as the expressions of TGF-β1, NF-κB and Hes1 (P<0.05 or P<0.01).


Brucine may inhibit osteoclastogenesis by suppressing Jagged1/Notch1 signaling pathways.


brucine breast cancer bone metastasis Jagged1/Notch1 signaling pathway 


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Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Ke-fei Hu
    • 1
  • Xiang-ying Kong
    • 2
  • Mi-cun Zhong
    • 2
  • Hong-ye Wan
    • 2
  • Na Lin
    • 2
  • Xiao-hua Pei
    • 3
  1. 1.Beijing University of Chinese MedicineBeijingChina
  2. 2.Institute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijingChina
  3. 3.Department of Scientific Research and EducationBeijing University of Chinese Medicine Third Affiliated HospitalBeijingChina

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