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Chinese Journal of Integrative Medicine

, Volume 24, Issue 7, pp 502–511 | Cite as

San-Cao Granule (三草颗粒) Ameliorates Hepatic Fibrosis through High Mobility Group Box-1 Protein/Smad Signaling Pathway

  • Shi-zhang Wei
  • Sheng-qiang Luo
  • Jian Wang
  • Jia-bo Wang
  • Rui-sheng Li
  • Xiao-mei Zhang
  • Yan-lei Guo
  • Chang Chen
  • Xiao Ma
  • Zhe Chen
  • Hong-hong Liu
  • Zhi-rui Yang
  • Jian-yu Li
  • Rui-lin Wang
  • Ya-ming Zhang
  • Hui-yin Yang
  • Xiao-he Xiao
  • Yan-ling ZhaoEmail author
Original Article

Abstract

Objective

To investigate the possible mechanism of San-Cao Granule (SCG, 三草颗粒) mediating antiliver fibrosis.

Methods

A total of 60 male Sprague-Dawley rats were randomly divided into the normal control group, porcine serum-treated group, ursodesoxycholic acid (UDCA, 60 mg/kg), SCG (3.6 g/kg) group, SCG (1.8 g/kg) group and SCG (0.9 g/kg) group, with 10 rats in each group. Liver fibrosis was induced with porcine serum by intraperitoneal injection for 8 weeks, except for the normal control group. Then, the rats in the three SCG-treated groups and UDCA group were administered SCG and UDCA respectively for 4 weeks. The serum levels of alanine transaminase (ALT), aspartate transaminase (AST), albumin (ALB), total bilirubin (TBIL), hyaluronic acid (HA), laminin (LN), and type IV collagen (IVC) were examined using commercial kits and hepatic histopathology was examined with hematoxylin and eosin and Masson staining. Moreover, the protein expression levels of high mobility group box-1 protein (HMGB1), transforming growth factor β1 (TGF-β1), phosphorylated mothers against decapentaplegic homolog 3 (p-Smad3), Smad7, toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), nuclear factor-kappa B (NF-κB) and α-smooth muscle actin (α-SMA) were determined by western blot, immunohistochemistry and real time quantitative-reverse transcription polymerase.

Results

Both SCG (3.6 and 1.8 g/kg) and UDCA significantly ameliorated the liver fibrosis induced by porcine serum as indicated by retarding the serum levels increasing of ALT, AST, TBIL, HA, LN and IVC and preventing the serum level reducing of ALB compared with the model group (all P<0.01). Meanwhile, the collagen deposition was attenuated by SCG and UDCA treatment. Furthermore, SCG markedly reduced the expressions of HMGB1, TGF-β1, p-Smad3, TLR4, MyD88, NF-κB and α-SMA, and enhanced the expression of the Smad7 compared with the model group (all P<0.01).

Conclusion

SCG ameliorates hepatic fibrosis possibly through inhibiting HMGB1, TLR4/NF-κB and TGF-β1/Smad signaling pathway.

Keywords

San-Cao Granule liver fibrosis high mobility group box-1 protein toll-like receptor 4/nuclear factor-kappa B transforming growth factor β1/mothers against decapentaplegic homolog 

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Supplementary material

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Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine 2015

Authors and Affiliations

  • Shi-zhang Wei
    • 1
    • 2
  • Sheng-qiang Luo
    • 3
  • Jian Wang
    • 1
  • Jia-bo Wang
    • 4
  • Rui-sheng Li
    • 5
  • Xiao-mei Zhang
    • 1
    • 6
  • Yan-lei Guo
    • 6
  • Chang Chen
    • 1
    • 2
  • Xiao Ma
    • 1
    • 2
  • Zhe Chen
    • 1
    • 2
  • Hong-hong Liu
    • 3
  • Zhi-rui Yang
    • 1
    • 2
  • Jian-yu Li
    • 3
  • Rui-lin Wang
    • 3
  • Ya-ming Zhang
    • 4
  • Hui-yin Yang
    • 3
  • Xiao-he Xiao
    • 4
  • Yan-ling Zhao
    • 1
    • 2
    Email author
  1. 1.College of PharmacyChengdu University of Traditional Chinese MedicineChengduChina
  2. 2.Department of Pharmacy302 Hospital of People’s Liberation ArmyBeijingChina
  3. 3.Department of Integrative Medical Center302 Hospital of People’s Liberation ArmyBeijingChina
  4. 4.China Military Institute of Chinese Medicine302 Hospital of People’s Liberation ArmyBeijingChina
  5. 5.Animal Laboratory Center302 Hospital of People’s Liberation ArmyBeijingChina
  6. 6.Chongqing Academy of Chinese Traditional Materia MedicaKey Laboratory of Chongqing Traditional Chinese Medicine ResourcesChongqingChina

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