Comparative effect of propolis of honey bee and some herbal extracts on Candida albicans
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To determine the effect of propolis on Candida albicans and to compare it with the effects of some other herbal extracts and antibiotics on this pathogenic fungi.
The extracts of propolis, Thymus vulgaris, Caryophillium aromaticus, Echinophora platyloba, Allium cepa and Cinnamomum zeylanicum were prepared and the antifungi effects of the extracts were examined on Candida albicans ATCC10231 using disc-diffusion assay and micro-broth dilution. The minimum fungicidal concentration (MFC) and minimum inhibitory concentrations (MIC) as well as inhibition zone were evaluated and the anti fungi effects of herbal extracts were compared with amphotricin B and nystatin at the times of 24, 48 and 72 h. Data analysis was performed using t test.
Obtained results showed that propolis extract with MIC90 and MFC equal to 39 and 65 μg/mL, respectively, possess the highest antifungal activity when compared with other studied extracts. The extracts of Allium cepa and Thymus vulgaris, with MFC of 169 and 137 μg/mL, respectively, showed the lowest effects on the fungi. Also nystatin and amphotricin B yielded better effects on the tested fungi compared with the effects of all studied extracts on Candida albicans.
Propolis extract is effective in controlling Candida albicans. However, the issue requires further investigation on samples in animals and performing toxicological examinations.
KeywordsCandida albicans propolis herbal extract antibiotics
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The authors would like to express their appreciation to Islamic Azad University of Khorasgan Branch for the valuable supports.
- 9.Nascimento GGF, Locatelli J, Freitas PC, Silva GL. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Braz J Microbiol 2000;31: 247–256.Google Scholar
- 10.Gavanji S, Larki B, Doostmohammadi M, Mortezaeinezad F. Production of a new mixed herbal medicine for minor aphthous Ulcers. Med Plants 2012;4: 49–51.Google Scholar
- 11.Kumar A, Shukla R, Singh P, Prasad CS, Dubey NK. Assessment of Thymus vulgaris L. essential oil as a safe botanical preservative against post harvest fungal infestation of food commodities. Innovat Food Sci Emerg Tech 2008;9: 575–580.Google Scholar
- 12.Griffin SG, Markham JL, Leach DN. An agar dilution method for the determination of the minimum inhibitory concentration of essential oils. J Essent Oil Res 2000;12: 249–255.Google Scholar
- 14.Agnihotri S, Vaidya A. A novel approach to study antibacterial properties of volatile components of selected Indian medicinal herbs. Ind J Exp Biol 1996;34: 712–715.Google Scholar
- 15.Gavanji S, Asgari MJ, Vaezi R, Larki B. Antifungal effect of the extract of propolis on the growth of three species of Epidermophyton flucosum, Trichophyton violaseum and Trichophytontonsorans in laboratory environment. Afr J Pharm Pharmacol 2011;5: 2642–2646.Google Scholar
- 16.Joe MM, Jayachitra J, Vijayapriya M. Antimicrobial activity of some common spices against certain human pathogens. J Med Plants Res 2009;3: 1134–1136.Google Scholar
- 19.Yadav A, Singh R. Natural preservatives in poultry meat products. Nat Prod Radiance 2004;3: 300–303.Google Scholar
- 22.Entezari M, Hashemi M, Ashki M, Ebrahimian S, Bayat M, Azizi Saraji A. Studying the effect of Echinophora Platyloba extract on bactira (Staphilococusaureus and Pseudomonas aeroginosa) and fungi (Candidiaalbicans, Aspergilusflavus and Aspergilusniger) in vitro. World J Med Sci 2009;4: 89–92.Google Scholar
- 23.Avijgan M, Hafizi M, Saadat M, Nilforoushzadeh MA. Antifungal effect of Echinophora Platyloba’s extract against Candida albicans. Iran J Pharm Res 2010;5: 285–289.Google Scholar
- 24.Bansod S, Rai M. Antifungal activity of essential oils from Indian medicinal plants against human pathogenic Aspergillusfumigatus and A. niger. World J Med Sci 2008;3: 81–88.Google Scholar
- 27.Lampe JW. Health effects of vegetables and fruit: assessing mechanisms of action in human experimental studies. Am J Clin Nutr 1990;70: 475–490.Google Scholar
- 33.Digrak M, Yilmaz O, Ozcelik S. In vitro antimicrobial effect of propolis collected in Elazig region. Turk J Biol 1995;19: 249–257.Google Scholar
- 35.Khosravi A, Behzadi A. Evaluation of the antibacterial activity of the seed hull of Quercusbrantii on some gram negative bacteria. Pak J Med Sci 2006;22: 429–432Google Scholar
- 44.Gavanji S, Larki B, Bakhtari A. The effect of extract of Punicagranatum var. pleniflora for treatment of minor recurrent aphthous stomatitis. Integr Med Res 2014;3: 83–90.Google Scholar
- 45.Akbari S. The survey antifungal effects of Thymus vulgaris L. and Origanum vulgare L. extracts against clinical isolates Candida albicans resistant and susceptible to fluconazole. J Med Plants 2006;6: 53–62.Google Scholar
- 46.Beytollahi J, Mansourian A, Esmaili M. Antimicrobial effect of propolis on common oral pathogen microorganisms (Candida albicans, Streptococcus mutans, Actinobacillus). J Den Soci 2010;21: 33–39.Google Scholar