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Chinese Journal of Integrative Medicine

, Volume 25, Issue 1, pp 45–50 | Cite as

Protective Effect of Zengye Decoction (增液汤) on Submandibular Glands in Nonobese Diabetic Mice

  • Cheng-yin Li
  • Su-ling Wu
  • Li-xia Sun
  • Ting-ting Yan
  • Yue WangEmail author
Original Article
  • 84 Downloads

Abstract

Objective

To investigate the protective effect of Zengye Decoction (增液汤, ZYD) on the submandibular glands (SMGs) in nonobese diabetic (NOD) mice.

Methods

Twenty-seven female NOD mice were randomly equally divided into 3 groups: the model group, the hydroxychloroquine (HCQ) group, and the ZYD group. Nine C57/B6 mice served as the normal group. After 1-week acclimation, the HCQ and ZYD groups were intragastrically administered with HCQ and ZYD, respectively, and the normal and model groups were administered with normal saline. Changes in the salivary flow rate were observed. Mice from all 4 groups were sacrificed at the age of 20 weeks. The serum and SMGs were collected. Serum cytokines gamma-interferon (IFN-γ), interleukin-10 (IL-10) were detected by enzyme-linked immunosorbent assay. Histological changes in the submandibular glands were examined by hematoxylin and eosin staining. The mRNA expression of IFN-γ, IL-10 and vasoactive intestinal peptide (VIP) in the submandibular glands were measured by real-time polymerase chain reaction.

Results

Compared with the model group, the salivary flow of the ZYD group significantly increased (P<0.05), the extent of the histological changes was ameliorated (P<0.05), and the Th1/Th2 cytokine imbalance was remedied (P<0.05). In the ZYD-treated mice, the VIP mRNA was up-regulated (P<0.05).

Conclusions

ZYD is beneficial in protecting structure and function of SMGs in NOD mice. The mechanism may be associated with the correction of the Th1/Th2 cytokine imbalance, and with the prevention of a progressive decline of the VIP level.

Keywords

Sjögren’s syndrome Th1/Th2 vasoactive intestinal peptide Zengye Decoction Chinese medicine 

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Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine 2014

Authors and Affiliations

  • Cheng-yin Li
    • 1
    • 2
  • Su-ling Wu
    • 3
  • Li-xia Sun
    • 1
  • Ting-ting Yan
    • 4
  • Yue Wang
    • 1
    Email author
  1. 1.The First College of Clinical MedicineNanjing University of Chinese MedicineNanjingChina
  2. 2.Department of RheumatologyChongqing Hospital of Traditional Chinese MedicineChongqingChina
  3. 3.Nanjing Hospital of Traditional Chinese MedicineNanjingChina
  4. 4.Institute for Ancient Texts of Traditional Chinese MedicineNanjing University of Chinese MedicineNanjingChina

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