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Chinese Journal of Integrative Medicine

, Volume 19, Issue 8, pp 573–581 | Cite as

Improved mesenchymal stem cell survival in ischemic heart through electroacupuncture

  • Jin Zhang (张 进)
  • Xiao-hua Jia (贾晓花)
  • Zhi-wei Xu (徐志伟)
  • Fu-ping Ding (丁富平)
  • Xin Zhou (周 欣)
  • Hao Fu (符 浩)
  • Yi Liu (刘 义)
  • Lai-liang Ou (欧来良)
  • Zong-jin Li (李宗金)
  • De-ling Kong (孔德领)
Original Article

Abstract

Objective

To investigate whether electroacupuncture (EA) can promote cell survival and enhance heart function of mesenchymal stem cells (MSCs) therapy.

Methods

MSCs were isolated from bone marrow and expanded in Minimum Essential Medium Alpha (α-MEM). MI was induced in 72 Sprague-Dawley (S-D) rats by ligation of the left anterior descending coronary artery (LAD) for 30 min and reperfusion. MI rats randomly received injection of 1×106 DiI-labeled MSCs alone (n =24, MSC group), or plus electroacupuncture (EA) at Neiguan (PC6, n=24, EA+MSC group), or saline (n =24, saline group). EA treatment was performed for 4 days. Another 24 rats were subjected to chest-open surgery without LAD occlusion and treatment (sham group). Three time points, 4, 14 and 28 days (n =8 for each group) were included in this study. The survival of transplanted MSCs and the protective gene expression were analyzed by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot at day 4 and 14. Left ventricular remodeling, cardiac function, infarction area, fibrosis and capillary density were analyzed at day 28.

Results

EA can enhance MSC survival (2.6-fold up) at day 4. Big capillary density was 53% higher in EA+MSC treated group than MSC alone group. Furthermore, the rats treated by EA reduced the fibrosis and had 36% smaller infarct size comparing to MSC alone. EA also attenuated left ventricular remodeling and enhanced the functional recovery of infarcted hearts at week 4.

Conclusion

EA at Neiguan acupoint can promote the stem cell survival and improve ischemic heart function. EA could become a useful approach in stem cell therapy for ischemia heart diseases.

Keywords

electroacupuncture Neiguan (PC6) mesenchymal stem cell myocardial ischemia apoptosis 

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Reference

  1. 1.
    Braunwald E, Bristow MR. Congestive heart failure: fifty years of progress. Circulation 2000;102:IV14–IV23PubMedGoogle Scholar
  2. 2.
    Lin JH, Shih CH, Kaphle K, Wu LS, Tseng WY, Chiu JH, et al. Acupuncture effects on cardiac functions measured by cardiac magnetic resonance imaging in a feline model. Evid Based Complement Alternat Med 2008;17:169–176.Google Scholar
  3. 3.
    Longhurst JC. Electroacupuncture treatment of arrhythmias in myocardial ischemia. Am J Physiol Heart Circ Physiol 2007;292:H2032–H2034.PubMedCrossRefGoogle Scholar
  4. 4.
    Li P, Pitsillides KF, Rendig SV, Pan HL, Longhurst JC. Reversal of reflex-induced myocardial ischemia by median nerve stimulation: a feline model of electroacupuncture. Circulation 1998;97:1186–1194.PubMedCrossRefGoogle Scholar
  5. 5.
    Lujan HL, Kramer VJ, DiCarlo SE. Electroacupuncture decreases the susceptibility to ventricular tachycardia in conscious rats by reducing cardiac metabolic demand. Am J Physiol Heart Circ Physiol 2007;292:H2550–H2555.PubMedCrossRefGoogle Scholar
  6. 6.
    Orlic D, Kajstura J, Chimenti S, Jakoniuk I, Anderson SM, Li B, et al. Bone marrow cells regenerate infarcted myocardium. Nature 2001;410:701–705.PubMedCrossRefGoogle Scholar
  7. 7.
    Djouad F, Plence P, Bony C, Tropel P, Apparailly F, Sany J, et al. Immunosuppressive effect of mesenchymal stem cells favors tumor growth in allogeneic animals. Blood 2003;102:3837–3844.PubMedCrossRefGoogle Scholar
  8. 8.
    Toma C, Pittenger MF, Cahill KS, Byrne BJ, Kessler PD. Human mesenchymal stem cells differentiate to a cardiomyocyte phenotype in the adult murine heart. Circulation 2002;105:93–98.PubMedCrossRefGoogle Scholar
  9. 9.
    Li Z, Han Z, Wu JC. Transplantation of human embryonic stem cell-derived endothelial cells for vascular diseases. J Cell Biochem 2009;106:194–199.PubMedCrossRefGoogle Scholar
  10. 10.
    Li Z, Lee A, Huang M, Chun H, Chung J, Chu P, et al. Imaging survival and function of transplanted cardiac resident stem cells. J Am Coll Cardiol 2009;53:1229–1240.PubMedCrossRefGoogle Scholar
  11. 11.
    Cheng Z, Ou L, Zhou X, Li F, Jia X, Zhang Y, et al. Targeted migration of mesenchymal stem cells modified with CXCR4 gene to infarcted myocardium improves cardiac performance. Mol Ther 2008;16:571–579.PubMedCrossRefGoogle Scholar
  12. 12.
    Hua XB. Acupuncture manual for small animals. In: Experimental acupunture. Shanghai: Shanghai Science and Technology Publisher; 1994:269–290.Google Scholar
  13. 13.
    Tang YL, Zhao Q, Qin X, Shen L, Cheng L, Ge J, et al. Paracrine action enhances the effects of autologous mesenchymal stem cell transplantation on vascular regeneration in rat model of myocardial infarction. Ann Thorac Surg 2005;80:229–236.PubMedCrossRefGoogle Scholar
  14. 14.
    Mangi AA, Noiseux N, Kong D, He H, Rezvani M, Ingwall JS, et al. Mesenchymal stem cells modified with Akt prevent remodeling and restore performance of infarcted hearts. Nat Med 2003;9:1195–1201.PubMedCrossRefGoogle Scholar
  15. 15.
    Makino S, Fukuda K, Miyoshi S, Konishi F, Kodama H, Pan J, et al. Cardiomyocytes can be generated from marrow stromal cells in vitro. J Clin Invest 1999;103:697–705.PubMedCrossRefGoogle Scholar
  16. 16.
    Ulett GA, Han J, Han S. Traditional and evidence-based acupuncture: history, mechanisms, and present status. South Med J 1998;91:1115–1120.PubMedCrossRefGoogle Scholar
  17. 17.
    Tsou MT, Huang CH, Chiu JH. Electroacupuncture on PC6 (Neiguan) attenuates ischemia/reperfusion injury in rat hearts. Am J Chin Med 2004;32:951–965.PubMedCrossRefGoogle Scholar
  18. 18.
    Li P, Tjen ALSC, Longhurst JC. Excitatory projections from arcuate nucleus to ventrolateral periaqueductal gray in electroacupuncture inhibition of cardiovascular reflexes. Am J Physiol Heart Circ Physiol 2006;290:H2535–H2542.PubMedCrossRefGoogle Scholar
  19. 19.
    Vogel JH, Bolling SF, Costello RB, Guarneri EM, Krucoff MW, Longhurst JC, et al. Integrating complementary medicine into cardiovascular medicine. A report of the American College of Cardiology Foundation Task Force on Clinical Expert Consensus Documents (Writing Committee to Develop an Expert Consensus Document on Complementary and Integrative Medicine). J Am Coll Cardiol 2005;46:184–221.PubMedCrossRefGoogle Scholar
  20. 20.
    Zhang J, Xu Z, Cheng Q. Stem cells and congenital essence of the basic theory of traditional Chinese medicine. Chin J Clin Rehab (Chin) 2006;10:189–192.Google Scholar
  21. 21.
    Yu P, Wang R, Liu M. Clinical application of the point Neiguan with illustrative case report. J Tradit Chin Med (Chin) 2007;27:180–182.Google Scholar

Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Jin Zhang (张 进)
    • 1
    • 2
  • Xiao-hua Jia (贾晓花)
    • 1
  • Zhi-wei Xu (徐志伟)
    • 2
  • Fu-ping Ding (丁富平)
    • 3
  • Xin Zhou (周 欣)
    • 4
  • Hao Fu (符 浩)
    • 1
  • Yi Liu (刘 义)
    • 1
  • Lai-liang Ou (欧来良)
    • 1
  • Zong-jin Li (李宗金)
    • 1
  • De-ling Kong (孔德领)
    • 1
  1. 1.State Key Laboratory of Medicinal Chemical Biology, College of Life ScienceNankai UniversityTianjinChina
  2. 2.School of Fundamental Medical SciencesGuangzhou University of Chinese MedicineGuangzhouChina
  3. 3.School of NursingGuangzhou University of Chinese MedicineGuangzhouChina
  4. 4.Institute of Cardiovascular Diseases, Pingjin HospitalMedical College of Armed Police ForcesTianjinChina

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