Chinese Journal of Integrative Medicine

, Volume 17, Issue 11, pp 847–853 | Cite as

Scavenging effect of Naoerkang (脑尔康) on amyloid beta-peptide deposition in the hippocampus in a rat model of Alzheimer’s disease

  • Xi Li (李 玺)
  • Hai-feng Yuan (袁海峰)
  • Qian-kun Quan (权乾坤)
  • Jian-jun Wang (王建军)
  • Ning-ning Wang (王宁宁)
  • Ming Li (李 明)
Original Article

Abstract

Objective

To observe the effect of a Chinese medicine compound, Naoerkang (脑尔康, NEK), on amyloid-beta peptide (1–42; Aβ1–42) and matrix metalloproteinase-9 (MMP-9) expressions in the hippocampus of Alzheimer’s disease (AD) model rats.

Methods

A total of 48 male Sprague Dawley (SD) rats were randomly divided into normal control, untreated, and piracetam groups, and low-dose, medium-dose, and high-dose NEK groups, with 8 rats in each group. The 5-μL aggregated Aβ1–42 (2 μg/μL) were injected into both CA1 areas of the hippocampus in the rats to establish an AD model, whereas the normal control was treated with the same dose of normal saline. The rats in the NEK groups were treated with a high, medium, or low dose of NEK [60 g/(kg·d), 30 g/(kg·d), and 15 g/(kg·d)], respectively, intragastrically for 28 days; piracetam (0.375 g/kg, intragastrically) was consecutively administered in the piracetam group; and normal saline was applied in the normal control and untreated groups. A Y-maze test was used for behavioral study to test the learning and memory abilities. Aβ1–42 and MMP-9 expressions in the hippocampus was determined immunohistochemically, and the results were analyzed by image acquisition and an analysis system.

Results

Aggregated Aβ1–42 induced obvious learning and memory dysfunction, as well as up-regulation of Aβ1–42 expression in the hippocampus. Compared with those in the normal control group, the learning and memory abilities of rats in the untreated group significantly decreased (P<0.01), and the expression of Aβ1–42 was significantly increased (P<0.01). Twenty-eight days after different treatments, compared with those in the untreated group, the learning and memory abilities of AD model rats in the piracetam, low-dose, medium-dose and high-dose NEK groups were significantly improved (P<0.01 or P<0.05), and the expression of Aβ1–42 in the hippocampus decreased (P<0.01 or P<0.05), and MMP-9 increased (P<0.01 or P<0.05), especially in the high-dose NEK group.

Conclusion

NEK might play a role of anti-dementia by increasing the expression of MMP-9 in the hippocampus of AD model rats, resulting in the reduction of the quantity of Aβ1–42 and improvement in learning and memory ability in AD model rats.

Keywords

Naoerkang Alzheimer’s disease amyloid beta-peptide matrix metalloproteinase-9 rats 

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Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Xi Li (李 玺)
    • 1
  • Hai-feng Yuan (袁海峰)
    • 2
  • Qian-kun Quan (权乾坤)
    • 3
  • Jian-jun Wang (王建军)
    • 4
  • Ning-ning Wang (王宁宁)
    • 1
  • Ming Li (李 明)
    • 1
  1. 1.Department of Geriatrics, the Second Affiliated Hospital, School of MedicineXi’an Jiaotong UniversityXi’anChina
  2. 2.Department of Neurology, the Second Affiliated Hospital, School of MedicineXi’an Jiaotong UniversityXi’anChina
  3. 3.Research Center of Rehabilitation Science and Technology, School of Life Science and TechnologyXi’an Jiaotong UniversityXi’anChina
  4. 4.Department of GeriatricsFuzhou General Hospital of Nanjing Military DistrictFuzhouChina

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