Correlation between Fc γ R III a and aortic atherosclerotic plaque destabilization in ApoE knockout mice and intervention effects of effective components of Chuanxiong Rhizome and Red Peony Root

  • Ye Huang (黄 烨)
  • Hui-jun Yin (殷惠军)
  • Xiao-juan Ma (马晓娟)
  • Jing-shang Wang (王景尚)
  • Qian Liu (刘 倩)
  • Cai-feng Wu (吴彩凤)
  • Ke-ji Chen (陈可冀)
Original Article

Abstract

Objective

To explore the correlation between Fc γ RIII A (CD16A) and aortic atherosclerotic plaque destabilization in apoE knockout (apoE KO) mice and the intervention effects of effective components of Chuanxiong Rhizome and Red Peony Root.

Methods

Eight 8-week-old male C57BL/6J mice were selected as the control group. Forty 8-week-old male apoE KO mice were randomly divided into the model group, apoE KO + intraperitoneal injection immunoglobulin group (IVIG), apoE KO + simvastatin group (Sm), apoE KO + high dosage of Xiongshao Capsule (XSC, 芎芍胶囊) group (XSCH), and apoE KO + low dosage of XSC group (XSCL), 8 mice in each group. Mice in the control group were put on a normal diet, and others were fed with a high-fat diet. After 10-week different interventions, monocyte CD16 expression was detected by flow cytometry, aortic matrix metalloproteinase-9 (MMP-9) mRNA expression was detected using reverse transcriptionpolymerase chain reaction, and serum tumor necrosis factor (TNF)-α level was detected using enzyme-linked immunosorbent assay.

Results

Compared with the control group, monocyte CD16 expression, aortic MMP-9 mRNA expression, and serum TNF-α level in the model group increased obviously (P<0.01). Injections of apoE KO mice with intraperitoneal immunoglobulin during a 5-day period significantly reduced the monocyte CD16 expression, aortic MMP-9 mRNA expression, and serum TNF-α level (P<0.01 or 0.05) over a 10-week period of high-fat diet. Indices above in the Sm group, XSCH group, and XSCL group decreased in a different degree. Of them, the aortic MMP-9 mRNA expression in XSCH group was lower than that in Sm group (P<0.05) and the monocyte CD16 expression and serum TNF-α level showed no significant difference between XSCH group and Sm group (P>0.05). Correlation analyses suggested positive correlation between monocyte CD16 expression and aortic MMP-9 mRNA expression or serum TNF-α level in IVIG group, XSCH group, and XSCL group.

Conclusions

FcγR III A mediates systemic inflammation in the progression of coronary heart disease with blood stasis syndrome. XSC could stabilize atherosclerotic plaque by suppressing inflammation and its target was relative with FcγRIII A.

Keywords

FcγRIIIA apoE knockout mice matrix metalloproteinase-9 tumor necrosis factor α effective components of Chuanxiong Rhizome and Red Peony Root 

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Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Ye Huang (黄 烨)
    • 1
  • Hui-jun Yin (殷惠军)
    • 1
  • Xiao-juan Ma (马晓娟)
    • 1
  • Jing-shang Wang (王景尚)
    • 1
  • Qian Liu (刘 倩)
    • 1
  • Cai-feng Wu (吴彩凤)
    • 1
  • Ke-ji Chen (陈可冀)
    • 1
  1. 1.Department of Cardiovascular Diseases, Xiyuan HospitalChina Academy of Chinese Medical SciencesBeijingChina

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