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Chinese Journal of Integrative Medicine

, Volume 16, Issue 6, pp 504–509 | Cite as

Effect of Erzhi Pill (二至丸) on improving cerebral nerve cell apoptosis in aging rats

  • Hui-li Gao (高会丽)
  • Dan-qiao Wang (王丹巧)Email author
  • Xiao-yan Wang (汪晓燕)
  • Zhi-guo Wang (王志国)
  • Xiang-ming Wang (王香明)
  • Zhao-en Wu (吴兆恩)
  • Hong Liu (刘 红)
  • Yan-yan Ma (马琰岩)
  • Xiao-hong Niu (牛晓红)
  • Lian-da Li (李连达)
Experimental Research

Abstract

Objective

To investigate the effects of Erzhi Pill (二至丸,EZP) on nerve cell apoptosis in senescence model rats.

Methods

The rats model of senescence was established by peritoneal D-galactose injection combined with thymusectomy. Forty SD rats were randomized into four groups, the normal control group, the senescence model group, the EZP treated group, and the vitamins treated group, 10 in each group. The rats were made into senescence model except those in the normal group. In the same time of D-galactose injection, the rats were treated respectively with distilled water, EZP 4.32 g/kg, and vitamins E and C 0.06 g/kg daily for 6 weeks via intragastric infusion. The index of main viscera (as brain, testis, etc.), serum levels of superoxide dismutase (SOD) activity, and total anti-oxidation capacity (T-AOC) were measured after a 6-week treatment. Meanwhile, the cerebral cortex neuronal apoptosis proportion and mitochondrial membrane potential (MMP) were detected by flow cytometry.

Results

Both EZP and vitamins E and C treatments showed effects on increasing testis index and serum level of T-AOC, reducing the percentage of neuronal apoptosis in the cerebral cortex, and elevating MMP in the aging rats model.

Conclusions

EZP could inhibit the cerebral cortex neuron apoptosis and maintain the mitochondrial function in the senescent process of rats induced by peritoneal D-galactose injection combined with thymusectomy. It also shows antioxidation effect to some extents.

Keywords

Erzhi Pill nerve cell apoptosis total antioxidation capacity senescence model rat 

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Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Hui-li Gao (高会丽)
    • 1
  • Dan-qiao Wang (王丹巧)
    • 1
    Email author
  • Xiao-yan Wang (汪晓燕)
    • 1
  • Zhi-guo Wang (王志国)
    • 1
  • Xiang-ming Wang (王香明)
    • 1
  • Zhao-en Wu (吴兆恩)
    • 1
  • Hong Liu (刘 红)
    • 1
  • Yan-yan Ma (马琰岩)
    • 1
  • Xiao-hong Niu (牛晓红)
    • 1
  • Lian-da Li (李连达)
    • 1
  1. 1.Medical Experimental CenterChina Academy of Chinese Medical SciencesBeijingChina

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