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Chinese Journal of Integrative Medicine

, Volume 16, Issue 4, pp 344–347 | Cite as

Effects of Yinian Jiangya Decoction (颐年降压饮) containing serum on cytokines secretion of vascular endothelium of spontaneously hypertensive rats

  • Yong-hua Zhao (赵永华)Email author
  • You-hua Xu (徐友华)
  • Ying Guan (关 莹)
  • Ping Xiang (项 平)
Experimental Research

Abstract

Objective

To observe the effects of Yinian Jiangya Decoction (颐年降压饮, YNJYD) on cytokine secretion in spontaneoulsy hypertensive rats (SHRs) vascular endothelium.

Methods

Aortic endothelial cells (ECs) were primarily cultured from SHRs; male SD rats were treated with different doses (high, medium, and low doses) of YNJYD, the blood was collected on the 21st day, and then, the serum was separated. ECs were cocultured with the serum for different time courses, and the culture supernatant concentrations of endothelin (ET)-1, nitric oxide (NO), tissue-type plasminogen activator (t-PA), and plasminogen activator inhibitor (PAI-1) were determined by ABC-ELISA methods.

Results

ET-1, NO, t-PA, and PAI-1 levels in endothelial cell culture supernatant were increased in a time-dependent manner; YNJYD could significantly elevate NO and t-PA expressions in ECs, while ET-1 and PAI-1 expressions were dramatically decreased; these effects of YNJYD were in a concentration-dependent manner.

Conclusion

The therapeutic effect of YNJYD on hypertension is attributed to its effect on regulating vessel dilation and blood coagulation, in which ET-1/NO and PAI-1/t-PA are two pairs of pivotal mediators.

Keywords

Yinian Jiangya Decoction endothelial cell cytokine 

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Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Yong-hua Zhao (赵永华)
    • 1
    Email author
  • You-hua Xu (徐友华)
    • 1
  • Ying Guan (关 莹)
    • 1
  • Ping Xiang (项 平)
    • 1
  1. 1.Faculty of Chinese MedicineMacau University of Science and TechnologyMacaoChina

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