Chinese Journal of Integrative Medicine

, Volume 15, Issue 6, pp 442–447

Imperatorin is responsible for the vasodilatation activity of Angelica Dahurica var. Formosana regulated by nitric oxide in an endothelium-dependent manner

  • Hong Nie (聂 红)
  • Lan-zhen Meng (孟兰贞)
  • Jiu-yao Zhou (周玖瑶)
  • Xiao-feng Fan (樊晓峰)
  • Yong Luo-
  • Guang-wen Zhang (张广文)
Experimental Research

DOI: 10.1007/s11655-009-0442-z

Cite this article as:
Nie, H., Meng, L., Zhou, J. et al. Chin. J. Integr. Med. (2009) 15: 442. doi:10.1007/s11655-009-0442-z

Abstract

Objective

To demonstrate the vasodilatation activity of the coumarin-containing Angelica dahurica var. formosana and to further analyze active components in the herb extracts.

Methods

(1) The vasodilatation effects induced by different extracts (cyclohexane, ethyl acetate, acetone, methanol, 95 % ethanol and water) of Angelica dahurica var. formosana on mouse thoracic aorta pre-contracted with phenylephrine were investigated. (2) The amount of imperatorin and isoimperatorin in each extract was measured by high-performance liquid chromatography. (3) The vasodilatation effects of imperatorin and isoimperatorin on mouse thoracic aorta were compared using the same in vitro method. (4) The vasodilatation mechanism of imperatorin in the mouse thoracic aorta pre-contracted with phenylephrine was studied using the methods of denuded endothelium, NG-nitro-L-arginine methylester (L-NAME, a nitric oxide synthase inhibitor), and propranolol.

Results

(1) The cyclohexane and ethyl acetate extracts of Angelica dahurica var. formosana decreased the maximal response of phenylephrine-induced mouse thoracic aorta contraction dose-dependently, with 50% inhibiting concentration (IC50) values of 35.3±12.4 mg/L and 40.5±12.0 mg/L, respectively. The vasodilatation effect of imperatorin and isoimperatorin was dose-dependent. (2) The cyclohexane extract, showing the strongest vasodilatation effect, possessed the highest contents of imperatorin (4.09%) and isoimperatorin (0.27%, w/w). There was a correlation between the vasodilatation activity and the contents of imperatorin and isoimperatorin in the extracts. (3) The vasodilatation effect of imperatorin was about 4-fold stronger than that of isoimperatorin. (4) The vasodilatation effect of imperatorin was signifificantly attenuated to 24.88%±4.06% in the denuded endothelium group compared with the intact endothelium group. And 1 mmol/L L-NAME reduced the imperatorin-induced vasorelaxation by 32.18 %±11.29 %.

Conclusions

The principal effective component of Angelica dahurica var. Formosana was found to be imperatorin. Imperatorin-induced vasodilatation is at least partially regulated by nitric oxide, and has no correlation to β-receptor.

Key Words

Angelica dahurica var. formosana thoracic aorta vasodilatation imperatorin isoimperatorin high-performance liquid chromatography 

Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine and Springer Berlin Heidelberg 2009

Authors and Affiliations

  • Hong Nie (聂 红)
    • 1
  • Lan-zhen Meng (孟兰贞)
    • 1
  • Jiu-yao Zhou (周玖瑶)
    • 2
  • Xiao-feng Fan (樊晓峰)
    • 3
  • Yong Luo-
    • 1
  • Guang-wen Zhang (张广文)
    • 4
  1. 1.College of PharmacyJinan UniversityGuangzhouChina
  2. 2.Guangzhou University of Chinese MedicineGuangzhouChina
  3. 3.Research Center for Analytical Sciences of Northeastern UniversityShenyangChina
  4. 4.College of Science and EngineeringJinan UniversityGuangzhouChina

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