Chinese Journal of Integrative Medicine

, Volume 15, Issue 2, pp 101–106 | Cite as

Differential gene expression profiles in coronary heart disease patients of blood stasis syndrome in traditional Chinese medicine and clinical role of target gene

  • Xiao-juan Ma (십쿾뻪)
  • Hui-jun Yin (틳믝뻼)
  • Ke-ji Chen (돂뿉벽)
Original Article

Abstract

Objective

To investigate the differential gene expression profiles in coronary heart disease (CHD) patients of blood-stasis syndrome (BSS) by oligonucleotide microarray technique, and the clinical significance of target gene.

Methods

Subjects were assigned to CHD patients with BSS (n=8), CHD patients without BSS (n=8), and BSS patients without CHD (n=8) based on coronary angiography and the diagnostic criteria of BSS. The sex- and age-matched healthy volunteers (n=8) were enrolled as the control group. Venous blood samples were collected for RNA extraction; Test-3 chip was employed to examine the quality of samples. Then, the samples were hybridized with Affymetrix U133 Plus 2.0 array to compare the gene expression profiles among the four groups. Gene-array scanner and gene chip operating software were applied to screen out hybridization signals and analyze gene expression, respectively. Based on the comparison of the samples of the four groups, the differential genes related with CHD and BSS were analyzed with Gene Ontology (GO) and pathway, and target genes selected were confirmed by real-time reverse transcription polymerase chain reaction (RT-PCR). Thirty CHD patients with BSS were selected according to the former criteria and 40 healthy as controls. The serum concentration of interleukin-8 (IL-8) was determined by double-antibody sandwich avidin-biotin peroxidase complex enzyme-linked (ABC-ELISA).

Results

A total of 107 differential genes were found being associated with CHD, including 48 up-regulated genes and 59 down-regulated genes. Among these 107 differential genes, 14 genes (13.1%) were found related to inflammatory reaction and immune response through GO analysis. In the pathway analysis, 4 of 15 conspicuous pathways were referred to the inflammation and immune response. Among 48 differential genes related to BSS, 26 genes were up-regulated, and 22 were down-regulated. Five of the 48 genes (10.4%) and 5 of 10 signficant pathways were involved in inflammation and immunity. The results of real-time RT-PCR proved the accuracy of the gene chip. The patients have markedly higher level of serum IL-8 compared to the controls (P<0.05).

Conclusion

The correlation of inflammatory- and immune-related genes with CHD patients of BSS was revealed at the level of nucleic acid, and the target gene IL-8 may play a role in the pathobiology of CHD with BSS.

Key Words

coronary heart disease blood-stasis syndrome differential gene expression profile target gene 

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References

  1. 1.
    Ma M, Zhang GJ. The progress of investigation on the objectification of blood stasis syndrome. J Shandong Univ Tradit Chin Med (Chin) 2002;2:155–158.Google Scholar
  2. 2.
    Xu H, Lu XY, Chen KJ, Shi DZ. Study on correlation of blood-stasis syndrome and its accompanied syndromes with pathological changes showed in coronary angiography and restenosis after percutaneous coronary intervention. Chin J Integr Tradit West Med 2007;27:8–13.Google Scholar
  3. 3.
    Special Committee of Promoting Blood Circulation and Removing Blood Stasis, Chinese Association of Integrative Medicine. The diagnostic criteria of blood stasis syndrome. Chin J Integr Tradit West Med (Chin) 1987;7:129.Google Scholar
  4. 4.
    Zhong S, Storch KF, Lipan O, Kao MC, Weita CJ, Wong WH. GoSurfer: a graphical interactive tool for comparative analysis of large gene sets in Gene Ontology space. Appl Bioinformat 2004;3:261–264.CrossRefGoogle Scholar
  5. 5.
    Shi YH, Zhu SW, Mao XZ, Feng JX, Qin YM, Zhang L, et al. Transcriptome profiling, molecular biological, and physiological studies reveal a major role for ethylene in cotton fiber cell elongation. Plant Cell (Chin) 2006;18:651–664.CrossRefGoogle Scholar
  6. 6.
    Mao X, Cai T, Olyarchuk JG, Wei L. Automated genome annotation and pathway identification using the KEGG Orthology (KO) as a controlled vocabulary. Bioinformatics 2005;21: 3787–3793.PubMedCrossRefGoogle Scholar
  7. 7.
    Brunetti ND, Correale M, Pellegrino PL, Cuculo A, Biase MD. Acute phase proteins in patients with acute coronary syndrome: Correlations with diagnosis, clinical features, and angiographic findings. Eur J Intern Med 2007;18:109–117.PubMedCrossRefGoogle Scholar
  8. 8.
    Napoleão P, Santos MC, Selas M, Viegas-Crespo Crespo AM, Pinheiro T, Ferreira RC. Variations in inflammatory markers in acute myocardial infarction: a longitudinal study. Rev Port Cart Cardiol 2007;26:1357–1363.Google Scholar
  9. 9.
    Davies MJ. The pathophysiology of acute coronary syndromes. Heart 2000;83:361–366.PubMedCrossRefGoogle Scholar
  10. 10.
    Wick G, Knoflach M, Xu Q. Autoimmune and inflammatory mechanisms in atherosclerosis. Annu Rev Immunol 2004;22:361–403.PubMedCrossRefGoogle Scholar
  11. 11.
    Chen KJ, Shi ZX. 1st ed. Practical workbook of blood-stasis syndrome. Beijing: People’s Medical Publishing House; 1999:5–8.Google Scholar
  12. 12.
    Ma XJ, Yin HJ, Chen KJ. Research progress of correlation between blood-stasis syndrome and inflammation. Chin J Integr Tradit West Med (Chin) 2007;27:669–672.Google Scholar
  13. 13.
    Qi X, Li J, Gu J, Li S, Dang Y, Wang T. Plasma levels of IL-8 predict early complications in patients with coronary heart disease after percutaneous coronary intervention. Jpn Heart J 2003;44:451–461.PubMedCrossRefGoogle Scholar
  14. 14.
    Poddar R, Sivasubramanian N, Dibello PM, Robinson K, Jacobsec DW. Homocysteine induces expression and secretion of monocyte chemoattractant protein-1 and interleukin-8 in human aortic endothelial cells implications for vascular disease. Circulation 2001;103:2717–2723.PubMedGoogle Scholar
  15. 15.
    Simonini A, Moscucci M, Muller DW, Bates ER, Pagani FD, Burdick MD, et al. IL-8 is an angiogenic factor in human coronary atherectomy tissue. Circulation 2000;101:1519–1526.PubMedGoogle Scholar
  16. 16.
    Guo M. Role of IL-8 in tumor angiogenesis. Chin J Gastroenterol Hepatol (Chin) 2003;12:409–411.Google Scholar

Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag GmbH 2009

Authors and Affiliations

  • Xiao-juan Ma (십쿾뻪)
    • 1
  • Hui-jun Yin (틳믝뻼)
    • 1
  • Ke-ji Chen (돂뿉벽)
    • 1
  1. 1.Department of Cardiovascular Disease, Xiyuan HospitalChina Academy of Traditional Chinese MedicineBeijingChina

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